Autoregulation of stress response in microorganisms

Nikolaev, Yu. A.; Mulyukin, A. L.; Stepanenko, I. Yu.; Эль-Регистан, Г. И.

doi:10.1134/s0026261706040096

Cited by 32 publications

(20 citation statements)

References 22 publications

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“…1e) and was particularly prominent with C12 and C18 AHB. The latter is due to the known growth inhibiting activity of these long chain AHBs [2,3]. In terms of our work, it should be emphasized that the inhibitory action of AHBs on the growth related characteristics of microorganisms was two orders of magnitude less significant than that associ ated with the lethal effect of UV radiation (see above).…”

Section: Resultsmentioning

confidence: 58%

“…High (10 -3 M) concentra tions of long chain AHBs that induce the anabiotic state of bacterial cells [2,18] brought about a pro nounced repression of the SOS response. This was due to transcription arrest in anabiotic cells [2,3]. It should be mentioned that the action of long chain alkylhydroxybenzenes at the post translational level results in inhibiting the functional activity of proteins [2,3,7,11].…”

Section: Resultsmentioning

confidence: 99%

“…They control the transition of recipient cells to the hypometabolic and anabiotic state [2]. Another aspect of the biological activity of AHBs is their functioning as adaptogens involved in developing of microbial resistance to a wide variety of deleterious factors [3]. In a number of studies, the protective effects of AHBs on the cells of bacterial and yeast cul tures under various kinds of stress [4,5], including UV irradiation [6], were demonstrated.…”

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confidence: 99%

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Involvement of alkylhydroxybenzenes in the Escherichia coli response to the lethal effect of UV irradiation

et al. 2012

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This work is concerned with the role of alkylhydroxybenzenes (AHBs), chemical analogs of the autoregulatory microbial d 1 factors, on the development of the stress response of bacterial cells to UV irradi ation, including SOS system induction, preservation of cell viability, and S R phase transitions of the Escherichia coli test strain with the bioluminescence genes cloned under the control of the recA gene pro moter. UV irradiation, a natural stress factor, and an increase in AHB concentrations were found to elicit uni form responses in bacteria, indicating that AHBs function as alarmones, i.e., alarm signals. It was revealed that preincubating bacteria with alkylhydroxybenzenes considerably enhanced their viability upon irradiation with lethal UV doses; this was accompanied by a relative decrease in the SOS response activity and a concom itant increase in the frequency of phase transitions. The efficiency of the protective action of AHBs increased with an increase in their hydrophobicity degree. The probable mechanism of the protective effect of AHBs is discussed, based on their capacity for the interaction with biopolymers, which results in changing their struc tural organization and conferring resistance to a broad spectrum of stress factors. Such a "passive" protective mechanism reduces the susceptibility of DNA to UV irradiation, causing a decrease in the parameters related to the SOS system induction that is responsible for the "active" protective mechanism in bacterial cells. As a result, viability retention under the lethal influence of UV irradiation is possible at minimal values of repair activity and is accompanied by an increase in the phenotypic variability of the surviving part of a bacterial population.

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Section: Resultsmentioning

confidence: 58%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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Involvement of alkylhydroxybenzenes in the Escherichia coli response to the lethal effect of UV irradiation

et al. 2012

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“…The cells might have aggregated to protect the population against stress conditions, resembling with a phenomenon of quoram sensing, where bacteria co-ordinate their behavior in response to stressful conditions. Nikolaev et al [16] and Vakhitov and Petrov [31] recently described the reversible adhesion of cells in submerged bacterial cultures under stress conditions and the processes of cell reactivation in the post-stress period in E. coli, Vibrio and few other organisms. Similarly, Khmel [10] reported that this communication was achieved by the production of small molecules (termed as autoinducers), which, at suYcient concentrations, trigger a variety of cellular responses.…”

Section: Discussionmentioning

confidence: 99%

“…Organic solvents can be extremely toxic to cells by virtue of their ability to partition into and disrupt the normal functioning of biological membranes. They accumulate in the bacterial cell membrane changing structural and functional integrity followed by cell lysis [9,16,27]. Organic solvent tolerant bacteria are a relatively novel group of extremophilic microorganisms with novel tolerance mechanisms, which enable them to overcome the toxic and destructive eVects of organic solvents in solvent-saturated environments [8,10,24,30,33].…”

Section: Introductionmentioning

confidence: 99%

Organic solvent tolerance of an alkaline protease from salt-tolerant alkaliphilic Streptomyces clavuligerus strain Mit-1

Thumar

Singh

2008

J Ind Microbiol Biotechnol

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A salt-tolerant alkaliphilic actinomycete, Mit-1 was isolated from Mithapur, coastal region of Gujarat, India. The strain was identified as Streptomyces clavuligerus and based on 16S rRNA gene sequence (EU146061) homology; it was related to Streptomyces sp. (AY641538.1). The organism could grow with up to 15% salt and pH 11, optimally at 5% and pH 9. It was able to tolerate and secrete alkaline protease in the presence of a number of organic solvents including xylene, ethanol, acetone, butanol, benzene and chloroform. Besides, it could also utilize these solvents as the sole source of carbon with significant enzyme production. However, the organism produced spongy cell mass with all solvents and an orange brown soluble pigment was evident with benzene and xylene. Further, the enzyme secretion increased by 50-fold in the presence of butanol. With acetone and ethanol; the enzyme was highly active at 60-80 degrees C and displayed optimum activity at 70 degrees C. The protease was significantly stable and catalyzed the reaction in the presence of xylene, acetone and butanol. However, ethanol and benzene affected the catalysis of the enzyme adversely. Crude enzyme preparation was more stable at 37 degrees C in solvents as compared to partially purified and purified enzymes. The study holds significance as only few salt-tolerant alkaliphilic actinomycetes are explored and information on their enzymatic potential is still scares. To the best of our knowledge this is the first report on organic solvent tolerant protease from salt-tolerant alkaliphilic actinomycetes.

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Biological activity of phenolic lipids

Stasiuk

Kozubek

2009

Cell. Mol. Life Sci.

228

158

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Phenolic lipids are a very diversified group of compounds derived from mono and dihydroxyphenols, i.e., phenol, catechol, resorcinol, and hydroquinone. Due to their strong amphiphilic character, these compounds can incorporate into erythrocytes and liposomal membranes. In this review, the antioxidant, antigenotoxic, and cytostatic activities of resorcinolic and other phenolic lipids are described. The ability of these compounds to inhibit bacterial, fungal, protozoan and parasite growth seems to depend on their interaction with proteins and/or on their membrane-disturbing properties.

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Autoregulation of stress response in microorganisms

Cited by 32 publications

References 22 publications

Involvement of alkylhydroxybenzenes in the Escherichia coli response to the lethal effect of UV irradiation

Involvement of alkylhydroxybenzenes in the Escherichia coli response to the lethal effect of UV irradiation

Organic solvent tolerance of an alkaline protease from salt-tolerant alkaliphilic Streptomyces clavuligerus strain Mit-1

Biological activity of phenolic lipids

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