1963
DOI: 10.3181/00379727-113-28529
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Autoradiography with the Agents of Psittacosis and Trachoma.

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Cited by 13 publications
(9 citation statements)
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“…The demonstration by Hatch (10) that C. psittaci draws on the ribonucleotide pools of its L-cell host for precursors for ribonucleic acid synthesis supports the concept that chlamydiae owe their obligatory intracellular mode of reproduction, at least in part, to requirements for preformed energy intermediates. However, several investigators (4,14,19,22) have observed that exogenous thymidine fails to enter the DNA of chlamydiae although it readily enters the DNA of host cells, which suggests that not all phosphorylated products of host metabolism are available to the parasite. In extensive autoradiographic studies, Crocker From these values, the nanomoles of total thymidine incorporated into L-cell and C. psittaci DNA was calculated, assuming that the mole percentage of thymidine in both L cells and C. psittaci is 30.…”
Section: Discussionmentioning
confidence: 99%
“…The demonstration by Hatch (10) that C. psittaci draws on the ribonucleotide pools of its L-cell host for precursors for ribonucleic acid synthesis supports the concept that chlamydiae owe their obligatory intracellular mode of reproduction, at least in part, to requirements for preformed energy intermediates. However, several investigators (4,14,19,22) have observed that exogenous thymidine fails to enter the DNA of chlamydiae although it readily enters the DNA of host cells, which suggests that not all phosphorylated products of host metabolism are available to the parasite. In extensive autoradiographic studies, Crocker From these values, the nanomoles of total thymidine incorporated into L-cell and C. psittaci DNA was calculated, assuming that the mole percentage of thymidine in both L cells and C. psittaci is 30.…”
Section: Discussionmentioning
confidence: 99%
“…Although exogenously supplied thymidine serves as a precursor of DNA in many different kinds of cells, autoradiograms of infected cells showed that H3-thymidine did not significantly label the DNA of several different mem bers of the psittacosis group (23,38,97,113) . Thymidine also failed to re verse the growth inhibition of aminopterin on a psittacosis agent (101) .…”
Section: Mouldermentioning
confidence: 96%
“…In this investigation, the activity of thymidine kinase and other enzymes related to DNA synthesis was studied in L cells infected with Chlamydia psittaci (strain meningopneumonitis). Thymidine kinase was singled out for intensive investigation, because it could be inferred from previous reports (2,34,47,53) of the failure of chlamydiae to incorporate exogenous thymidine I Present address: Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada. into their DNA that these microorganisms have no thymidine kinase of their own. Therefore, in view of the well-known relationship between thymidine kinase activity and DNA synthesis in mammalian cells (3,27,48), it appeared likely that the mechanism of inhibition of DNA synthesis in meningopneumonitis-infected L cells could be studied by use of the thymidine kinase activity of the L-cell host as an indicator.…”
mentioning
confidence: 99%