Autoradiographische Bestimmung der S-Phasen-Dauer der Gonocyten bei der Wistarratte durch Einfach- und Doppelmarkierung

Hilscher, B.; Hilscher, W; Delbrück, G.; Lerouge-Bénard, B.

doi:10.1007/bf00306791

Cited by 22 publications

(10 citation statements)

References 20 publications

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“…Relatively large prespermatogonia, found mostly as single cells in the center of the testicular tubules, are the bovine equivalent to the resting T 1 -prospermatogonia described in rodents (B Hilscher et al 1972(B Hilscher et al , 1974Miething 1993). Different, however, from the pattern reported for the rat and golden hamster, and probably due to the long time span of their occurrence (from day 80 p.c.…”

Section: Discussionmentioning

confidence: 76%

“…As in the rat and golden hamster (B Hilscher et al 1972(B Hilscher et al , 1974W Hilscher and B Hilscher 1976;Miething 1993Miething , 1998, the bovine male germ cell population also shows periods of different proliferative activity in the time span between testicular cord formation in the embryo and the onset of spermatogenesis in the pubertal animal. A period of high proliferation rate is observed from day 50 p.c.…”

Section: Discussionmentioning

confidence: 93%

“…In a number of small rodents the prepubertal germ cells undergo clear-cut phases of proliferation or mitotic quiescence (B Hilscher et al 1972(B Hilscher et al , 1974W Hilscher and B Hilscher 1976;W Hilscher 1991;Miething 1993Miething , 1998: Two waves of enhanced proliferation (M-and T 2 -prospermatogonia) are described which are separated by a generation of non-proliferating cells (T 1 -prospermatogonia). This scheme of prespermatogenesis, found in the rat and golden hamster, could not be corroborated by studies of the human prenatal testis.…”

Section: Introductionmentioning

confidence: 98%

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Prespermatogenesis and spermatogoniogenesis in the bovine testis

Wrobel¹

2000

Anatomy and Embryology

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The bovine male germ cell population was studied over the entire period from testicular differentiation in the embryo through onset of spermatogenesis in the pubertal calf. Germ cells were identified by protein gene product 9.5 immunohistochemistry and characterized by their ultrastructure. The proliferation pattern of germ cells was studied with immunohistochemical anti-Ki 67 and anti-proliferating cell nuclear antigen reactions. Germ cells with a high proliferation rate are observed from day 50 p.c. to day 80 p.c. These cells are in transition from primordial germ cells to prespermatogonia. From day 80 p.c. until approximately the 15th postnatal week the germ cells present are identified as prespermatogonia. From day 80 p.c. to day 200 p.c. germ cell multiplication decreases continuously; then the prespermatogonia enter a phase of relative mitotical quiescence that lasts until the 4th postnatal week. Between the 4th and the 15th postnatal week, testicular tubular diameters grow from 40 to 80 microm and the prespermatogonia resume their proliferation. In seminiferous tubules with diameters between 80 and 120 microm, found in animals between 18 and 27 weeks of age, a central lumen is normally still absent. During this period germ cell proliferation reaches a second maximum. The cells involved represent the members of the spermatogonia stem and precursor cell line kinetically interpolated between the prespermatogonia and the first differentiating A-spermatogonia. This second phase of prepubertal germ cell multiplication coincides with the period when the pre-Sertoli cells transform into adult-type Sertoli cells and enter the G0-phase for the rest of life.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 98%

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Prespermatogenesis and spermatogoniogenesis in the bovine testis

Wrobel¹

2000

Anatomy and Embryology

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“…Interestingly, Hilscher et al [29,[67][68][69][70] made the point that the morphology of M-prospermatogonia is initially similar to that of oogonia at the equivalent developmental period, and that sexual dimorphism between developing male and female germ cells is really first visible on the basis of distinctions between T 1 -prospermatogonia, which enter mitotic arrest, and primary oocytes, which have entered first meiotic prophase at the equivalent developmental stage. However, in a later publication, Hilscher and Hilscher [72] noted that a more subtle distinction between male and female germ cell development may be seen slightly earlier, at the end the first proliferation wave of M-prospermatogonia and oogonia, respectively, when the cell cycle is seen to be slightly shorter in male germ cells than in female germ cells [73].…”

Section: Preferable Terminology For Fetal and Neonatal Male Germ Cellmentioning

confidence: 99%

“…A nomenclature that avoids all of the deficiencies of the gonocyte terminology described above was introduced in a MCCARREY series of studies published in the 1970s by Hilscher et al [29,[67][68][69][70]. These authors made the case that male germ cell development can be divided into three major phases, which they termed the ''pregonadal period'' (the period of PGC specification and migration to the genital ridges), ''prespermatogenesis'' (a phase during fetal and neonatal development in male rodents characterized by proliferation and differentiation of the precursors of the mature germ cells later found in the adult testis), and ''spermatogenesis'' (the maturation of the male germ cells to form spermatozoa in the pubertal and adult testis).…”

Section: Preferable Terminology For Fetal and Neonatal Male Germ Cellmentioning

confidence: 99%

Toward a More Precise and Informative Nomenclature Describing Fetal and Neonatal Male Germ Cells in Rodents1

McCarrey

2013

Biology of Reproduction

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The germ cell lineages are among the best characterized of all cell lineages in mammals. This characterization includes precise nomenclature that distinguishes among numerous, often subtle, changes in function or morphology as development and differentiation of germ cells proceed to form the gametes. In male rodents, there are at least 41 distinct cell types that occur during progression through the male germ cell lineage that gives rise to spermatozoa. However, there is one period during male germ cell development-that which occurs immediately following the primordial germ cell stage and prior to the spermatogonial stage-for which the system of precise and informative cell type terminology is not adequate. Often, male germ cells during this period are referred to simply as "gonocytes." However, this term is inadequate for multiple reasons, and it is suggested here that nomenclature originally proposed in the 1970s by Hilscher et al., which employs the terms M-, T1-, and T2-prospermatogonia, is preferable. In this Minireview, the history, proper utilization, and advantages of this terminology relative to that of the term gonocytes are described.

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Ultrastructure of germ cells and sertoli cells in the postnatal rabbit testis

1973

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Spermatogenesis begins in the rabbit at seven to eight weeks of age. During the preceding postnatal period, seminiferous tubules contain round to oval prespermatogonia arranged in pairs at the tubular periphery and elongated Sertoli cells lying perpendicular to the basal lamina. Prespermatogonia are distinguished from fetal gonocytes by their abundant cytoplasm and the presence of intercellular bridges. Preceding the onset of spermatogenesis, prespermatogonia undergo diminution in cell size and cytoplasmic condensation. At the same time there is marked nucleolar enlargement, with elaborate proliferation of the nucleolonema. By the end of the seventh week, characteristic type A and B spermatogonia are present. Spermatocytes are seen at the end of the eighth week. Spermatids appear in the twelfth week, coincident with tubular lumen formation.

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Autoradiographische Bestimmung der S-Phasen-Dauer der Gonocyten bei der Wistarratte durch Einfach- und Doppelmarkierung

Cited by 22 publications

References 20 publications

Prespermatogenesis and spermatogoniogenesis in the bovine testis

Prespermatogenesis and spermatogoniogenesis in the bovine testis

Toward a More Precise and Informative Nomenclature Describing Fetal and Neonatal Male Germ Cells in Rodents1

Ultrastructure of germ cells and sertoli cells in the postnatal rabbit testis

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