Autoradiographische Bestimmung der Dauer der DNS-Verdopplung und ihres zeitlichen Verlaufs bei Spermatogonien der Ratte durch Doppelmarkierung mit C-14- und H-3-Thymidin

Hilscher, W; Maurer, W.

doi:10.1007/bf01185126

Cited by 76 publications

(17 citation statements)

References 2 publications

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“…The Ts of astroglial cells was determined with the double labelling method after [3H]dT and [14C]dT application, as introduced by Maurer and co-workers (Hilscher & Maurer 1962, Pilgrim & Maurer 1962 and Wimber & Quastler (1963).…”

Section: Duration Of S Phasementioning

confidence: 99%

Autoradiographic studies of rat astroglial cell proliferation in vitro with and without treatment with basic fibroblast growth factor

et al. 1992

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Using specific autoradiographic methods, cell cycle parameters of untreated and basic fibroblast growth factor (bFGF)-treated astroglial cells from newborn rats grown in primary culture were directly measured. The mode of proliferation was also analysed. In untreated cultures, S phase duration (Ts = 6.9-13.1 h) and cell cycle time (Tc = 10-18 h) can be modified by about a factor of 2 depending on the culture conditions (serum-supplemented or defined medium, thyroid hormone concentration). However, growth fraction (GF = 0.15) and the ratio Ts/Tc remain stable. With increasing days in vitro (DIV) (DIV 7-DIV 20), Ts (7.8-10.6 h) and Tc (10-21 h) are prolonged and GF (0.14-0.06) decreases, probably due to cell maturation. In general, astroglial cells proliferate exponentially with a GF < 1, but stop proliferating about 30-36 h after the last feeding, probably caused by exhaustion of the medium. However, after refeeding they continue to proliferate. As opposed to in vivo, no transition of non-proliferating cells into the GF occurs. After addition of bFGF, GF increases (e.g. GF at DIV 7 = 0.43), but Ts and Tc are not influenced at DIV 7 and 12. At DIV 20, bFGF additionally shortens Ts and Tc, thereby producing values of Ts, Tc and GF like 'younger' cultures. However, the revitalizing effect on 'mature' cells is only transitory. In general, bFGF leads to a single re-entry of G0 cells into the GF. Thereafter, bFGF does not affect the mode of proliferation.

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Section: Duration Of S Phasementioning

confidence: 99%

Autoradiographic studies of rat astroglial cell proliferation in vitro with and without treatment with basic fibroblast growth factor

et al. 1992

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“…To determine the length of S phase we used the double-labeling technique first introduced by Hilscher and Maurer [13] and Wimber and Quastler [45].…”

Section: Double-laheling Procedurementioning

confidence: 99%

The initial accumulation of carbamoylphosphate synthetase in embryonic rat hepatocytes, and the cell cycle

et al. 1989

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“…For brevity the convention will be adopted of designating these categories as (C + H)-labelled cells, (H)-labelled cells, (C)-labelled cells, and non-labelled cells. * Double labelling with 3H-and 14C-thymidine was first employed for the determination of S-phase durations (Hilscher & Maurer, 1962;Pilgrim & Maurer, 1962;Wimber & Quastler, 1963). This determination of S-phase durations is relatively simple, since it requires only the discrimination between (H)-labelled cells on the one hand and (C + H)or (C)-labelled cells on the other hand.…”

Section: Introductionmentioning

confidence: 99%

A Two Emulsion Autoradiographic Technique and the Discrimination of the Three Different Types of Labelling After Double Labelling With 3h‐ and Wc‐thymidine

1976

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The first part of the paper deals with a two emulsion autoradiographic technique for double labelling experiments with 3H‐ and 14C‐thymidine which permits a clear discrimination of the different types of labelling. In the second part the application of this technique to cell kinetic studies is discussed. Accurate discrimination between the different types of labelling, namely purely 3H‐, purely 14C‐ and double (3H +14C) labelling, is only possible if the activity ratio of 3H‐ to 14C‐thymidine is sufficiently high. This condition is necessary for a reliable distinction between those grains in the first emulsion which are due to true 3H‐labelling and spurious grains which are simultaneously produced in the same emulsion by 14C‐β‐particles. Experiments are described to determine the required activity ratio of 3H‐ to 14C‐thymidine.

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Autoradiographische Bestimmung der Dauer der DNS-Verdopplung und ihres zeitlichen Verlaufs bei Spermatogonien der Ratte durch Doppelmarkierung mit C-14- und H-3-Thymidin

Cited by 76 publications

References 2 publications

Autoradiographic studies of rat astroglial cell proliferation in vitro with and without treatment with basic fibroblast growth factor

Autoradiographic studies of rat astroglial cell proliferation in vitro with and without treatment with basic fibroblast growth factor

The initial accumulation of carbamoylphosphate synthetase in embryonic rat hepatocytes, and the cell cycle

A Two Emulsion Autoradiographic Technique and the Discrimination of the Three Different Types of Labelling After Double Labelling With 3h‐ and Wc‐thymidine

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