Autoradiographic Study on the Incorporation of Carbon-14 Labeled Formate and Adenine into Nucleic Acid in Blood-forming Cells

Adenine uptake by reticulum cells in the lymphoid tissues of rats.

“…With respect to this problem, our earlier observations are of particular interest. We have demonstrated that the monocyte precursors in the bone marrow, such as promonocytes or monoblasts, also exhibit especially strong DNA labeling with [14C]A, whereas the precursor cells of other blood cells do not (9,10). It should be added here that the labeling intensity of the monocyte precursors is comparable to that of the BLRC observed in this study.…”

“…In contrast, our previous study has revealed that the incorporation of [14C]adenine ([14C]A) by non-lymphoid cells such as proliferative reticulum cells takes place to a much greater extent than that by lymphoid cells (9). It has also been shown that the precursor cells of monocytes in the bone marrow, such as promonocytes or monoblasts, are much more intensely labeled with [14C]A than those of other blood cells, particularly following RNase treatment (9,10).…”

The use of (14C)adenine for autoradiographic labeling of blood monocytes and their precursor cells in the bone marrow of rats.

“…After three daily injections of [14C]A, the majority of pecursor cells of monocytes as well as those of granulocytes in the bone marrow were heavily labeled in contrast to weaker labeling of erythroblasts and lymphocytes, as previously reported (8) . The silver grains of the heavily labeled cells were reduced in number to some extent by pretreatment with RNase, but the cells still remained heavily labeled (Fig.…”

Adenine uptake by lamina propria cells in the intestinal mucosa of rats and mice, with special reference to reticulum cells.

“…However, the occurrence of such cells could not be verified by the present experiments. Finally, the authors wish to add that the monocyte precursors in the bone marrow of rats, and a portion of blood monocytes as well, were as intensely labeled with [14C]A as were the above-mentioned LPC, whereas other types of blood-forming cells were not (12,13). This suggests a possibility that the LPC exhibiting intense labeling with…”

“…However, our previous study has revealed that the incorporation of [14C]adenine ([14C]A) into nucleic acid, particularly DNA, by the monocyte precursors in the bone marrow takes place to a much greater degree than that by the precursor cells of other types of blood cells (13,14).…”