Autoradiographic study of 3H‐proline incorporation by rat periodontal ligament, gingival connective tissue and dental pulp

Abstract

The rates of 3-H-proline incorporation by the rat periodontal ligament, the gingival connective tissue and the dental pulp were studied by autoradiography. The rate of 3-H-proline incorporation by the periodontal ligament was 2.8 times higher than by the gingival connective tissue and 5 times higher than by the dental pulp. These differences were significant (p smaller than 0.01). The pattern of 3-H-proline incorporation by the periodontal ligament was significantly different (p smaller than 0.01) from that by the gingival connective tissue and the dental pulp. The latter two tissues exhibited a similar pattern of 3-H-proline incorporation. The ratio of the rates of 3-H-proline incorporation by the three tissues did not correlate with the ratio of the cellular densities in the same three tissues.

Identification of connective tissue components in healing rat gingiva

“…Thus, it takes about 10 days to reestablish continuity in the wound, since fibrillogenesis occurs in the wound within 4 days postinjury (9), This observation corresponds with the healing sequence following incision in skin as well as fiber orientation in in vitro models (2,13,16), indicating that simple incision in transseptal fibers may provide a standardized and reliable experimental model in the study of collagen fiber regeneration. Thus, it takes about 10 days to reestablish continuity in the wound, since fibrillogenesis occurs in the wound within 4 days postinjury (9), This observation corresponds with the healing sequence following incision in skin as well as fiber orientation in in vitro models (2,13,16), indicating that simple incision in transseptal fibers may provide a standardized and reliable experimental model in the study of collagen fiber regeneration.…”

“…Bridging of the wound space was in progress in 12-day post-injury specimens and appeared complete 14 days postoperatively. Thus, it takes about 10 days to reestablish continuity in the wound, since fibrillogenesis occurs in the wound within 4 days postinjury (9), This observation corresponds with the healing sequence following incision in skin as well as fiber orientation in in vitro models (2,13,16), indicating that simple incision in transseptal fibers may provide a standardized and reliable experimental model in the study of collagen fiber regeneration. After tooth replantation or flap surgerj', the transseptal fibers require a considerably longer period of healing to reach a comparable level of organization (3,5,7), Following incision in rat gingiva, a distinct wound space with wound edges is easily located from 1 to 10 days post-injury (9).…”

“…AceOular areas frequently found in healing tissue are usually taken to represent "old" tissue segments (5,6). Since normal rat gingiva incorporates radiotracers in the same way as periodontal ligament, although to a significantly lesser extent (16), it was decided to use autoradiographic labeling in an effort to identify tissue components. Thus, only the acellular part was assigned to preexisting tissue.…”

Dentin formation in formocresol pulpotomized primary monkey teeth studied by tetracycline and 3H‐proline incorporation

“…Apart from the apical area of one root all sections showed double labeling in the dentin (Figs. [3][4][5]. in seven out of 11 successfully treated roots labeling could even be demonstrated in the dentin adjacent to the level of amputation paste (Fig.…”

A Reevaluation of the Distribution of ³H-Proline in the Transseptal Ligament of the Mouse in Radioautography

“…Skougaard et al (1970a. b], Kameyama [1975] and Minkoff and Engstrom [1979], in radioautographic studies, suggest that the rate of collagen turnover is slower in the transseptal than in the periodon tal ligament. Conversely.…”