Autoradiographic mapping of neurotransmitter system receptors in mammalian brain

Araki, Tsutomu; Kato, Harubumi; Kogure, Kyuya; Shuto, Katsuro; Ishida, Yukiharu

doi:10.1016/0091-3057(92)90369-q

Cited by 16 publications

(5 citation statements)

References 20 publications

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“…Alternatively, alterations in GRs might not be directly responsible for the effect on behavior. For instance, the behavioral effects could be the result of an interaction between glucocorticoid and NMDA receptors, which are both plentiful in the interpositus nucleus (Araki et al, 1992) and important for learning-related plasticity (Chen and Steinmetz, 2000). Given the evidence for learning-related changes as a result of NMDA-GR interactions in the amygdala and hippocampus (Marginos and McEwen, 1995;Kim et al, 1996;Shors and Mathew, 1998;Roozendaal et al, 2003), these receptors may interact to produce the deficits in eyeblink conditioning in our study.…”

Section: Effects Of Maternal Separation On Gr Expressionmentioning

confidence: 78%

Neonatal maternal separation alters adult eyeblink conditioning and glucocorticoid receptor expression in the interpositus nucleus of the cerebellum

Wilber

Southwood

Sokoloff

et al. 2007

Developmental Neurobiology

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Neonatal maternal separation alters learning and memory. Glucocorticoids also modulate adult learning and memory, and neonatal maternal separation alters forebrain glucocorticoid receptor (GR) concentrations. We used eyeblink classical conditioning to assess the effect of neonatal maternal separation on associative learning. We assessed delay eyeblink conditioning, GR expression, and total neuron number in the interpositus nucleus, a critical site of plasticity in eyeblink conditioning, in adult rats that had undergone either standard animal facilities rearing, handling for 15 min, or maternal separation for either 15 or 60 min per day on postnatal days 2-14. At 2-3 months of age, delay eyeblink classical conditioning was assessed. Brains were processed for GR immunohistochemistry, and GR expression in the interpositus nucleus was assessed using a computer-based densitometry system. Neuron counts and nuclear volumes were obtained from an alternate series of thionin-stained sections. Maternal separation significantly impaired eyeblink conditioning in male but not female rats. Handling and maternal separation did not significantly affect interpositus neuron number and volume. However, prolonged maternal separation significantly increased GR expression in the posterior interpositus in males, and increases were correlated with eyeblink conditioning. In female rats, maternal separation and handling did not significantly alter interpositus neuron number, volume, or GR protein expression, and GR expression did not correlate with eyeblink conditioning. Thus, neonatal maternal separation produces adult deficits in eyeblink conditioning and alterations in GR expression in its neural substrate in a sex-dependent manner.

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Section: Effects Of Maternal Separation On Gr Expressionmentioning

confidence: 78%

Neonatal maternal separation alters adult eyeblink conditioning and glucocorticoid receptor expression in the interpositus nucleus of the cerebellum

Wilber

Southwood

Sokoloff

et al. 2007

Developmental Neurobiology

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“…The GABA A receptor consists of three binding sites. There are ligands which bind to GABA binding site (muscimol) [33,46], benzodiazepine binding site (FNZ etc.) [34,47], and chloride channels (TBPS) [35].…”

Section: Discussionmentioning

confidence: 99%

Autoradiographic Analysis of GABAA Receptors in μ-Opioid Receptor Knockout Mice

Tien

Fan

et al. 2007

Neurochem Res

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Anatomical evidence indicates that gamma-aminobutyric acid (GABA)-ergic and opioidergic systems are closely linked and act on the same neurons. However, the regulatory mechanisms between GABAergic and opioidergic system have not been well characterized. In the present study, we investigated whether there are changes in GABA(A) receptors in mice lacking mu-opioid receptor gene. The GABA(A) receptor binding was carried out by autoradiography using [(3)H]-muscimol (GABA(A)), [(3)H]-flunitrazepam (FNZ, native type 1 benzodiazepine) and [(35)S]-t-butylbicyclophosphorothionate (TBPS, binding to GABA(A)-gated chloride channels) in brain slices of wild type and mu-opioid receptor knockout mice. The binding of [(3)H]-FNZ in mu-opioid receptor knockout mice was significantly higher than that of the wild type controls in most of the cortex and hippocampal CA1 and CA2 formations. mu-Opioid receptor knockout mice show significantly lower binding of [(35)S]-TBPS than that of the wild type mice in few of the cortical areas including ectorhinal cortex layers I, III, and V, but not in the hippocampus. There was no significant difference in binding of [(3)H]-muscimol between mu-opioid receptor knockout and wild type mice in the cortex and hippocampus. These data indicate that there are specific regional changes in GABA(A) receptor binding sites in mu-opioid receptor knockout mice. These data also suggest that there are compensatory up-regulation of benzodiazepine binding site of GABA(A) receptors in the cortex and hippocampus and down-regulation of GABA-gated chloride channel binding site of GABA(A) receptors in the cortex of the mu-opioid receptor knockout mice.

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“…Placental mammals show only a slight high-to-low dorsolateral-to-ventromedial neostriatal gradient of indirect BG system elements, and the opposite gradient of direct BG elements, with extensive overlap [Regidor and Poch, 1988;Dietl and Palacios, 1988a, b;Gerfen et al, 1990;Araki et al, 1992;Hall et al, 1994;Parent and Hazrati, 1995a, b;Voogd et al, 1998]. The anole may represent a laminar extreme of a general tendency for an association of dorsal neostriatum with indirect BG system elements, and the ventral neostriatum with the direct BG system.…”

Section: Discussionmentioning

confidence: 99%

“…Sections then underwent 2 rinses in 0.1 M PBS (5 minutes), dehydrated in ethanol, and coverslipped with Permount tributions reported in mammals ( fig. 3) and birds [Dietl and Palacios, 1988a, b;Camps et al, 1990;Araki et al, 1992]. Dopamine D 2 receptors, on the other hand, had a markedly different distribution pattern.…”

Section: Peptide Antibody Localizationmentioning

confidence: 99%

Mammal-Like Striatal Functions in <i>Anolis</i>

Lr²,

Ls³

et al. 2000

Brain Behav Evol

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We used in situ autoradiographic ligand binding methods to determine the occurrence and distribution of dopamine D₁ and D₂ receptor sub-types in the anole lizard, Anolis carolinensis. Both were present and exhibited pharmacological specificity characteristics similar to those described for mammals. However, unlike in mammals where in the neostriatum [outside the nucleus accumbens/olfactory tubercle complex (NA/OT)] these receptors exhibit only slight dorsolateral (D₂ high, D₁ low) to ventromedial (D₁high, D₂ low) gradients that co mingle extensively, in the anole striatum outside the NA/OT there was a striking laminar pattern, with little if any overlap between D₂ (high in a dorsal band) and D₁ (high ventral to the D₂ band) distributions. As D₁ receptors are related to the direct and D₂ to the indirect basal ganglia (BG) subsystems in mammals, we also determined anole striatal distributions of pre-proenkephalin mRNA, a marker for striatal efferents to the indirect BG subsystem in mammals. Here, too, there was a striking laminar pattern, with pre-proenkephalin mRNA in a band similar to that seen for D₂ receptors. The crisp neuroanatomical separation between these classic BG subsystem markers in Anolis striatum make this species attractive for the study of such systems'' functions during behavior.

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Autoradiographic mapping of neurotransmitter system receptors in mammalian brain

Cited by 16 publications

References 20 publications

Neonatal maternal separation alters adult eyeblink conditioning and glucocorticoid receptor expression in the interpositus nucleus of the cerebellum

Neonatal maternal separation alters adult eyeblink conditioning and glucocorticoid receptor expression in the interpositus nucleus of the cerebellum

Autoradiographic Analysis of GABAA Receptors in μ-Opioid Receptor Knockout Mice

Mammal-Like Striatal Functions in <i>Anolis</i>

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