Autoradiographic localization of [3H]oestradiol in epididymis, seminal vesicles and prostate of the fetal guinea‐pig

Hatier, R; Malaprade, D; Roux, M.; Nguyen, B.-L.; Grignqn, G.; Pasqualini, Jörge R.

doi:10.1111/j.1365-2605.1990.tb00971.x

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…Thus, ERa and ERb are present in both stromal and epithelial tissues of the prostate during various developmental and adult stages. However, it is notable that steroid autoradiographic studies in various species and different developmental stages have consistently demonstrated the binding of 3 H-estradiol in the prostatic stroma but not in the epithelium (Cooke et al, 1991b;Hatier et al, 1990;Weaker and Sheridan, 1982). As described above, the induction of squamous metaplasia in prostatic epithelium is dependent upon ERa (and not ERb), and ERa is required simultaneously in both the epithelium and the stroma (Risbridger et al, 2001b).…”

Section: Hormonal Carcinogenesis In the Prostate Is Independent Of Epmentioning

confidence: 88%

“…Estrogens act through two specific receptors, ERa and ERb (Couse and Korach, 1999;Kuiper et al, 1996). However, it is notable that steroid autoradiographic studies in various species and different developmental stages have consistently demonstrated the binding of 3 H-estradiol in the prostatic stroma but not in the epithelium (Cooke et al, 1991b;Hatier et al, 1990;Weaker and Sheridan, 1982). ERb, which was originally cloned from prostatic tissue, is expressed at high levels in both prostatic epithelium and stroma in the developing prostate.…”

Section: Hormonal Carcinogenesis In the Prostate Is Independent Of Epmentioning

confidence: 99%

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Role of stroma in carcinogenesis of the prostate

2002

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Section: Hormonal Carcinogenesis In the Prostate Is Independent Of Epmentioning

confidence: 88%

Section: Hormonal Carcinogenesis In the Prostate Is Independent Of Epmentioning

confidence: 99%

Role of stroma in carcinogenesis of the prostate

2002

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“…In this regard, in recent autoradiographic studies we found that in the fetal rat uterus, radioactive estra¬ diol was located mainly in the stromal cells (24). Ennis & Stumpf (25) demonstrated that estradiol and 4-hydroxytamoxifen can differentially affect progestin binding sites among the uterine tissue compartments of the immature rat, but for these two compounds the main interactive region is in the stroma.…”

Section: Discussionmentioning

confidence: 95%

Biological responses of ICI 164,384 and other anti-estrogens in vaginal and uterine cells of fetal guinea pig in culture

Chétrite¹,

Pasqualini²

1991

Acta Endocrinologica

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The biological response of the anti-estrogens, ICI 164,384, 4-hydroxytamoxifen and tamoxifen were studied in isolated cells of the vagina and uterus of fetal guinea pig in culture. ICI 164,384 in these two cell lines provokes a significant stimulatory effect on the progesterone receptor levels. This is in opposition to available information where this compound is considered to have full anti-estrogenic properties. At the concentration of 10\m=-\8 mol/l ICI 164,384 stimulates progesterone receptor 2-3 times in relation to the non-treated cells. 4-hydroxytamoxifen acts as an agonist in the vaginal cells, but as an antagonist in the uterine cells, in which it can block the stimulatory effect provoked by ICI 164,384. Progesterone, which inhibits progesterone receptor, can block in both cells the stimulatory action of ICI 164,384. No effect of the different compounds tested was found in proliferation or [3H]thymidine incorporation. The agonistic effect of the anti-estrogen ICI 164,384 adds new information about the complexity of the mechanism of antiestrogen action and gives additional models to explore the biological responses of these compounds.The utilization of anti-estrogens has opened a new era in the investigation of the control of estrogen action. Owing to its lack of toxicity, one of these drugs, tamoxifen, is one of the anti-estrogens most frequently used as additive hormonal therapy in breast cancer (1,2). However, in different animal species, anti-estrogen can act as either a weak or a full estrogen. This is a function of the animal spe¬ cies, the experimental conditions, and the para¬ meter considered (reviewed in 3,4).Recently, ICI Pharmaceuticals (Macclesfield, UK) synthesized an estradiol derivative, ICI 164,384, which in different models acts as a full anti-estrogen (5,6). The use of isolated cells in cul¬ ture of the hormonal target organs is of great value in exploring the hormone and anti-hormone re¬ sponses.In this laboratory, we recently isolated fetal uter¬ ine cells of the guinea pig in which we studied the effect of tamoxifen and its derivatives (7). In the present work we isolated fetal vaginal cells of the same species and explored the effect of the new anti-estrogen ICI 164,384 on proliferation, DNA synthesis, and progesterone receptor on these two kinds of isolated cells. Materials and MethodsBiological materials and chemicals Pregnant guinea pigs of the albino Dunkin-Hartley strain (50-65 days of gestation) were purchased from Shamrock Bio Services (Gambais, France). Tissue culture medium (Dulbecco's Modified Eagle's Medium (DMEM)), phos¬ phate buffer saline (calcium-and magnesium-free) (PBS), fetal calf serum (FCS), L-glutamine, and HEPES were obtained from Flow Laboratories S.A. (Irvine, Scotland). Hanks buffered saline solution (calcium-and magne¬ sium-free) (HBSS), penicillin-streptomycin, and trypan blue were obtained from Gibco Europe Ltd (Paisley, Scot¬ land). Collagenase (Type I), trypsin (Type III), DNase I (Type I) and DMEM in powdered form without phenol red, were purchased fro...

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Evidence That Epithelial and Mesenchymal Estrogen Receptor-α Mediates Effects of Estrogen on Prostatic Epithelium

Risbridger

Wang

Young

et al. 2001

Developmental Biology

161

134

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In combination with androgens, estrogens can induce aberrant growth and malignancy of the prostate gland. Estrogen action is mediated through two receptor subtypes: estrogen receptors alpha (ERalpha) and beta (ERbeta). Wild-type (wt) and transgenic mice lacking a functional ERalpha (alphaERKO) or ERbeta (betaERKO) were treated with the synthetic estrogen diethylstilbestrol (DES). DES induced prostatic squamous metaplasia (SQM) in wt and betaERKO but not in alphaERKO mice, indicating an essential role for ERalpha, but not ERbeta, in the induction of SQM of prostatic epithelium. In order to determine the respective roles of epithelial and stromal ERalpha in this response, the following tissue recombinants were constructed with prostatic epithelia (E) and stroma (S) from wt and ERKO mice: wt-S+wt-E, alphaERKO-S+alphaERKO-E, wt-S+alphaERKO-E, and alphaERKO-S+wt-E. A metaplastic response to DES was observed in wt-S+wt-E tissue recombinants. This response to DES involved multilayering of basal epithelial cells, expression of cytokeratin 10, and up-regulation of the progesterone receptor. Tissue recombinants containing alphaERKO-E and/or -S (alphaERKO-S+alphaERKO-E, wt-S+alphaERKO-E, and alphaERKO-S+wt-E) failed to respond to DES. Therefore, full and uniform epithelial SQM requires ERalpha in the epithelium and stroma. These results provide a novel insight into the cell-cell interactions mediating estrogen action in the prostate via ERalpha.

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Autoradiographic localization of [3H]oestradiol in epididymis, seminal vesicles and prostate of the fetal guinea‐pig

Cited by 5 publications

References 13 publications

Role of stroma in carcinogenesis of the prostate

Role of stroma in carcinogenesis of the prostate

Biological responses of ICI 164,384 and other anti-estrogens in vaginal and uterine cells of fetal guinea pig in culture

Evidence That Epithelial and Mesenchymal Estrogen Receptor-α Mediates Effects of Estrogen on Prostatic Epithelium

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