Autoradiographic investigation of the effect of 1-hydroxyethylidene-1, 1-bisphosphonate on matrix protein synthesis and secretion by secretory ameloblasts in rat incisors

Fuangtharnthip, Pornpoj; Yamada, Yuya; Takagi, Yasuaki; Ohya, Keiichi

doi:10.1016/s0003-9969(00)00009-1

Cited by 9 publications

(17 citation statements)

References 32 publications

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“…The available experimental studies investigating the effects of bisphosphonates on tooth formation have been made by examining rat incisors Yamada et al 2000), which display continuous growth and eruption. The analysis of rat molar tooth germs carried out here offers the advantage of having the several stages of odontogenesis at a same slope of a given developing cusp.…”

Section: The Journal Of Histochemistry and Cytochemistrymentioning

confidence: 99%

Immunocytochemical Study of Amelogenin Deposition during the Early Odontogenesis of Molars in Alendronate-treated Newborn Rats

Massa

Bradaschia-Corrêa

Arana-Chavez

2006

J Histochem Cytochem.

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S U M M A R Y Newborn rats were treated with sodium alendronate to study how enamel is formed and the effect of alendronate during early odontogenesis. Ultrastructural analysis combined with high-resolution immunocytochemistry for amelogenin was carried out. Twelve rats were subjected to daily SC injections of sodium alendronate (2.5 mg/kg/day) for 3 days on their dorsal region, whereas three rats were daily injected with saline solution as a control. Molar tooth germs from 3-day-old rats were fixed under microwave irradiation in 0.1% glutaraldehyde 1 4% formaldehyde buffered at pH 7.2 with 0.1 M sodium cacodylate. The specimens were left undecalcified, postfixed with osmium tetroxide, dehydrated, and embedded in LR White resin. Ultrathin sections were incubated with a chicken anti-24-kDa rat amelogenin antibody, a secondary antibody, and finally with a protein A-gold complex. Large patches of amelogenin were present over the unmineralized mantle dentin and at early secretory ameloblasts. At more advanced stages, they were also detected at the enamel matrix, as well as in the mineralized dentin, at the periodontoblastic space of the dentinal tubules, and at the predentin. It is likely that the main effect of alendronate at early stages of odontogenesis is the increase of synthesis/secretion of amelogenin, promoting its deposition within the forming dentin and enamel. (J Histochem Cytochem 54:713 -725, 2006)

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Section: The Journal Of Histochemistry and Cytochemistrymentioning

confidence: 99%

Immunocytochemical Study of Amelogenin Deposition during the Early Odontogenesis of Molars in Alendronate-treated Newborn Rats

Massa

Bradaschia-Corrêa

Arana-Chavez

2006

J Histochem Cytochem.

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“…Bisphosphonates therapeutically act by causing toxic effects on osteoclasts or interfering with specific intracellular pathways in those cells [23][24][25]. Defective enamel mineralization is one of the possible adverse effects of these drugs [26][27][28][29][30][31]. Although bisphosphonates' mechanism of action is exhaustively studied in bone, their effects on the enamel formation are still poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Birefringence of the Secretory-Stage Enamel Organic Extracellular Matrix from Rats Submitted to Successive Injections of Bisphosphonates

Santo

Frozoni

Ramos‐Perez

et al. 2010

Connective Tissue Research

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The aim of the present study was to assess birefringence of the secretory-stage enamel organic extracellular matrix (ECM) and mechanical properties of mature enamel from rats treated with bisphosphonates. Longitudinal sections were obtained from upper incisors of rats that had been submitted to injections of bisodic etidronate (8 mg/Kg/day), sodium alendronate (30 microg/Kg/day), or sodium chloride as control (8 mg/Kg/day) for 42 days. Sections were immersed in 80% glycerin for 30 min and optical retardation of birefringence brightness in the secretory-stage enamel organic ECM was determined in nanometers. Etidronate-treated rats exhibited extensive morphological changes in the secretory-stage enamel organic ECM inclusive nonbirefringent conspicuous incremental lines, but presented optical retardation values similar to those showed by control rats (p > 0.05). Birefringence of secretory enamel organic ECM from etidronate rats presented an irregular aspect. Alendronate-treated rats did not show morphological alterations in the secretory-stage enamel organic ECM, however, they presented significant reduction in optical retardation of birefringence brightness when compared with control and etidronate rats (p < 0.01). Alendronate and etidronate groups exhibited reductions of approximately 6-10% in mature enamel cross-sectional microhardness when compared with control group (p < 0.01). Scanning electron microscopy analysis showed extensive alterations in mature enamel only from etidronate group with absence of enamel rods. The present work shows that bisphosphonates can affect the birefringence of the secretory-stage enamel organic ECM. The results presented here suggest that alterations in the supramolecular organization of the secretory-stage enamel organic ECM are a plausible mechanism by which environmental factors may cause enamel defects.

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“…Resultado similar foi encontrado por Fuangtharnthip et al (2000), após observarem que o etidronato não afeta a atividade ameloblástica na síntese de proteína, comprovando que a hipoplasia de esmalte em ratos tratados com etidronato pode não estar relacionada a um distúrbio na síntese protéica.…”

Section: Discussionunclassified

“…Além disso, nos estudos que verificaram alterações nas estruturas dentárias, após uso do etidronato, (Ogawa et al, 1989;Josephsen et al, 1990;Fejerskov et al, 1990;Weile et al, 1990;Fuangtharnthip et al, 2000;Yamada et al, 2000), o regime utilizado foi a injeção de uma dose única ou a administração diária da droga durante um curto intervalo de tempo.…”

Section: Discussionunclassified

“…O etidronato é um bisfosfonato não aminado, diferente do alendronato, um aminobisfosfonato, que possui elevada afinidade mineral e potente atividade antirreabsortiva, dada a sua capacidade de afetar processos celulares bioquímicos, como a inibição da enzima farnesil pirofosfato sintase (FPPS) (Russell et al, 2008). Tais características podem ser utilizadas também para justificar os resultados do presente estudo, que divergem da maioria dos achados de outros pesquisadores, que demonstraram diversos distúrbios nos tecidos dentários mineralizados, incluindo hipomineralização, hipoplasias e cistos, quando utilizaram o etidronato (Beertsen et al, 1985;Simmelink, 1987;Ogawa et al, 1989;Fejerskov et al, 1990;Josephsen et al, 1990;Weile et al, 1990;Fouda et al, 1991;Weile et al, 1993;Takano et al, 1998;Sakai et al, 1999;Fuangtharnthip et al, 2000;Yamada et al, 2000;Ohma et al, 2000). No presente estudo, não foi possível observar qualquer efeito significativo do alendronato sódico na formação do esmalte e da dentina de incisivos de ratos, por meio das metodologias empregadas.…”

Section: Discussionunclassified

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Efeito do uso sistêmico de alendronato sódico no tecido ósseo e nas estruturas dentárias mineralizadas: estudo químico, mecânico e morfológico, em modelo murino

Lucisano¹

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Autoradiographic investigation of the effect of 1-hydroxyethylidene-1, 1-bisphosphonate on matrix protein synthesis and secretion by secretory ameloblasts in rat incisors

Cited by 9 publications

References 32 publications

Immunocytochemical Study of Amelogenin Deposition during the Early Odontogenesis of Molars in Alendronate-treated Newborn Rats

Immunocytochemical Study of Amelogenin Deposition during the Early Odontogenesis of Molars in Alendronate-treated Newborn Rats

Birefringence of the Secretory-Stage Enamel Organic Extracellular Matrix from Rats Submitted to Successive Injections of Bisphosphonates

Efeito do uso sistêmico de alendronato sódico no tecido ósseo e nas estruturas dentárias mineralizadas: estudo químico, mecânico e morfológico, em modelo murino

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