Autoradiographic evidence for insulin and insulin-like growth factor binding to early mouse embryos

Mattson, B. A.; Rosenblum, Ira; Smith, Robert M.; Heyner, Susan

doi:10.2337/diabetes.37.5.585

Cited by 59 publications

(33 citation statements)

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“…It has been shown previously that insulin binds and stimulates mouse embryos soon after compaction (Harvey and Kaye 1988;Mattson et al 1988) and that inner cell mass cells from mouse blastocyst outgrowths bind IGF-I, IGF-II, and insulin (Mattson et al 1988). This indicates that one or more members of the insulin family of receptors must be functional during preimplantation mouse embryo development.…”

Section: Receptors For Igf-ii Function In Preimplantation Mouse Embryosmentioning

confidence: 97%

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Insulin-like growth factor II acts through an endogenous growth pathway regulated by imprinting in early mouse embryos.

Rappolee¹,

Sturm²,

Behrendtsen³

et al. 1992

Genes Dev.

274

180

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We present evidence that insulin-like growth factor II (IGF-II) mediates growth in early mouse embryos and forms a pathway in which imprinted genes influence development during preimplantation stages, mRNA and protein for IGF-II were expressed in preimplantation mouse embryos, but the related factors IGF-I and insulin were not. IGF-I and insulin receptors and the IGF-II/mannose-6-phosphate receptor were expressed. Exogenous IGF-II or IGF-I increased the cell number in cultured blastocysts, but a mutant form of IGF-II that strongly binds only the IGF-II receptor did not. Reduction of IGF-II expression by antisense IGF-II oligonucleotides decreased the rate of progression to the blastocyst stage and decreased the cell number in blastocysts. Preimplantation parthenogenetic mouse embryos expressed mRNA for the IGF-II receptor but not for either IGF-II ligand or the IGF-I receptor, indicating that the latter genes are not expressed when inherited maternally. These data imply that some growth factors and receptors, regulated by genomic imprinting, may control cell proliferation from the earliest stages of embryonic development.

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Section: Receptors For Igf-ii Function In Preimplantation Mouse Embryosmentioning

confidence: 97%

“…Early mouse embryos bind insulin, which affects their rate of protein synthesis in culture (Harvey and Kaye 1988;Mattson et al 1988). The insulin family includes insulin-like growth factor (IGF)-I, IGF-II, and insulin (two nonallelic genes) (Sara and Hall 1990).…”

mentioning

confidence: 99%

Insulin-like growth factor II acts through an endogenous growth pathway regulated by imprinting in early mouse embryos.

Rappolee¹,

Sturm²,

Behrendtsen³

et al. 1992

Genes Dev.

274

180

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show abstract

“…Embryos were examined daily from embryonic day (E) 7.5, corresponding to four or five pairs of somites (15) to E17.5. Adult mice were perfused through the heart with fixative solution; the pancreas was removed and post-fixed for 1 30 min, (iii) an empirically derived optimal dilution of control serum or primary antibody raised in species "X" containing 1% goat serum in TS for 18 hr, (iv) a 1:50 dilution of anti-(species x) biotinylated IgG solution in 1% goat serum in TS for 30 min, and (v) a 1:100 dilution of peroxidase-avidin complex for 30 min. After these incubations, the bound peroxidase was visualized by reaction for 6 min in a solution containing 22 mg of 3,3'-diaminobenzidine (DAB) and 10 ,ul of 30% H202 in 100 ml of 0.1 M Tris solution.…”

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confidence: 99%

A transgene coding for a human insulin analog has a mitogenic effect on murine embryonic beta cells.

Vincent¹,

Carroll²,

Hammer³

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

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We have investigated the mitogenic effect of three mutant forms of human insulin on insulin-producing f3 cells of the developing pancreas. We examined transgenic embryonic and adult mice expressing (i) Insulin is a peptide hormone that plays a crucial role in the control of blood glucose. It contains two separate polypeptide chains, the A chain containing 21 amino acid residues and the B chain containing 30 residues, which are linked to each other by a pair of disulfide bonds (1). Insulin is synthesized by ,3 cells of the pancreatic islets in the form of a precursor called preproinsulin (1), which is processed into proinsulin in the rough endoplasmic reticulum and transported via a regulated secretory pathway to storage granules, where it is cleaved to yield insulin and a 31-residue fragment called the C peptide. A small amount of proinsulin (0.5-2%) escapes from this route and is released through a constitutive, unregulated pathway (1-4). The expression of insulin solely by ,B cells has been controversial (5, 6). However, since we previously documented the expression of a chimeric gene containing the insulin I promoter in neurons of transgenic mice (7), the possibility was raised that, in addition to ,3 cells, insulin may be expressed by other cell types but at concentrations that are below the level of detection of the immunohistochemical techniques. To test this possibility, we initiated an analysis of transgenic mice overexpressing a human insulin analog. The transgene codes for the expression of a proinsulin molecule in which the amino acid histidine in position 10 of the B chain was replaced by aspartic acid. The substitution at B10 produced an aberrant intracellular sorting of the mutant hormone ([AspBlO]-proinsulin) resulting in secretion of up to 15% of the humanThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact. 6239[AspBlO]proinsulin via an unregulated pathway (8). However, processing of mouse and human proinsulin within secretory granules in the islets of transgenic mice was not impaired, and the residual mouse and most of the human proinsulin were converted to insulin and secreted normally (1). Surprisingly, this mutant form of insulin has a 4-to 5-fold higher biological activity than normal insulin (9, 10). The appearance of the vaginal plug was considered to be day 0.5 of gestation. Pregnant females were killed by cervical dislocation, the embryos were dissected, the trunk was processed for Southern blotting analysis, and the head and abdomen were fixed in 4% paraformaldehyde buffered to pH 7.4 with 0.1 M sodium phosphate buffered saline (PBS) for 1 hr. Embryos were examined daily from embryonic day (E) 7.5, corresponding to four or five pairs of somites (15) to E17.5. Adult mice were perfused through the heart with fixative solution; the pancreas was removed and post-fixed for 1 hr in

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“…While insulin effects on gene expression and presence of insulinrelated epitopes have been demonstrated in sea urchin embryos, the insulin receptor has not been characterized (8). Preimplantation mouse embryos (9) and rat embryos in early organogenesis (10) display insulin receptors with typical ligand-binding characteristics. However, it is not known if the 8 subunit of these receptors is functional.…”

mentioning

confidence: 99%

In situ autoradiography and ligand-dependent tyrosine kinase activity reveal insulin receptors and insulin-like growth factor I receptors in prepancreatic chicken embryos.

Girbau

Bassas

Alemany

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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We previously reported specific cross-linking of '2SI-labeled insulin and 12SI-labeled insulin-like growth fac- The signal transduction capability of the receptors in early organogenesis was assessed by their ability to phosphorylate the exogenous substrate poly(Glu8OTyr2'). Ligand-dependent tyrosine phosphorylation was demonstrable with both insulin and IGF-I in glycoprotein-enriched preparations from embryos at days 2 through 6 of embryogenesis. There was a developmentally regulated change in ligand-dependent tyrosine kinase activity, with a sharp increase from day 2 to day 4, in contrast with a small increase in the ligand binding. Binding of 12SI-labeled IGF-I was, with the solubilized receptors, severalfold higher than binding of 125I-labeled insulin. However, the insulin-dependent phosphorylation was as high as the IGF-

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Autoradiographic evidence for insulin and insulin-like growth factor binding to early mouse embryos

Cited by 59 publications

References 0 publications

Insulin-like growth factor II acts through an endogenous growth pathway regulated by imprinting in early mouse embryos.

Insulin-like growth factor II acts through an endogenous growth pathway regulated by imprinting in early mouse embryos.

A transgene coding for a human insulin analog has a mitogenic effect on murine embryonic beta cells.

In situ autoradiography and ligand-dependent tyrosine kinase activity reveal insulin receptors and insulin-like growth factor I receptors in prepancreatic chicken embryos.

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