Autoradiographic demonstration of DNA replication in ultrathin sections of plastic-embedded tissues using an exogeneous DNA polymerase

Geuskens, Maurice; Recondo, A M de; Chevaillier, Philippe

doi:10.1007/bf00326266

Cited by 5 publications

(1 citation statement)

References 34 publications

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“…In addition to 3H-thymidine incorporation studies, in which only the fraction of DNA replicated during the period of time when radioactive precursor was available to the cell is visualized, there are cytochemical methods allowing localization of DNA directly on ultrathin sections. These employ tritiated actinomycin D binding (Bernier et al 1972;Geuskens, 1974), terminal transferase catalysed labelling with 3H-dATP (Fakan & Modak, 1973a, b), or DNA polymerase catalysed incorooration of labelled nucleoside triphosphates (Geuskens de et al, 1975).…”

Section: Wphy (A) L O C a L I Z A T I O N O F D N Amentioning

confidence: 99%

High‐resolution autoradiography as a tool for the localization of nucleic acid synthesis and distribution in the mammalian cell nucleus

Fakan¹

1976

Journal of Microscopy

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Several examples of the application of high resolution autoradiography to the study of nucleic acid distribution in ultrathin sections of fixed and plastic embedded or frozen material are presented. Newly-synthesized DNA, labelled by very short pulses of 3H-thymidine is found to be localized throughout the nucleus. In the blastomeres of early mouse embryos developing after fertilization by 3H-thymidine-labelled spermatozoa, the labelled paternal DNA is distributed non-homogeneously in the nucleus. Finally some results obtained by using a cytochemical enzymatic technique for visualization of DNA directly in ultrathin sections of fixed and embedded cells are demonstrated. Concerning the distribution of RNA, perichromatin localization of rapidly transcribed RNA is described in different cell systems. This label can be attributed to perichromatin fibrils. In cells labelled for longer periods of time, sometimes followed by prolonged postincubations, a residual label is always found over the nucleus. Clusters of interchromatin granules are usually only weakly labelled or unlabelled. These structures probably contain a limited amount of rather slowly labelled RNA. The present results are discussed in the context of some biochemical evidence and of the data described by other investigators.

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Section: Wphy (A) L O C a L I Z A T I O N O F D N Amentioning

confidence: 99%

High‐resolution autoradiography as a tool for the localization of nucleic acid synthesis and distribution in the mammalian cell nucleus

Fakan¹

1976

Journal of Microscopy

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New approaches to in situ detection of nucleic acids

Thiry

1995

Histochem Cell Biol

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The present paper reviews recent results obtained by different molecular biology-based, immunocytological approaches to the localization and identification of nucleic acids in sections of biological material. Examples of sensitive, high-resolution detection methods for RNA, DNA or specialized DNA regions are presented. Special emphasis is placed on the potential values and limitations of these new methods.

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Ultrastructural Methods for Nucleic Acid Detection by Immunocytology

Thiry

1999

Progress in Histochemistry and Cytochemistry

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Autoradiographic demonstration of DNA replication in ultrathin sections of plastic-embedded tissues using an exogeneous DNA polymerase

Cited by 5 publications

References 34 publications

High‐resolution autoradiography as a tool for the localization of nucleic acid synthesis and distribution in the mammalian cell nucleus

High‐resolution autoradiography as a tool for the localization of nucleic acid synthesis and distribution in the mammalian cell nucleus

New approaches to in situ detection of nucleic acids

Ultrastructural Methods for Nucleic Acid Detection by Immunocytology

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