Autopolyploid formation of Trichoderma reesei QM9414 by colchicine treatment

Filter paper degrading ability of a Trichoderma strain with multinucleate conidia

“…Colchicine has been shown to induce polyploidy in plants by inhibiting mitosis (2,3). Colchicine has also been shown to induce polyploidy in fungi and Basidiomycetes (4,5). When fungal conidia are incubated in a liquid medium containing colchicine, the diameter of nuclei in conidia gradually increase and generate polyploid nuclei.…”

“…As the basic medium, Mandels' medium (NH 4 ) 2 SO 4 (Wako); 1.4 g, KH 2 PO 4 (Wako); 2.0 g, urea (Wako); 0.3 g, CaCl 2 (Wako); 0.3 g, MgSO 4 · 7H 2 O (Wako); 0.3 g, FeSO 4 · 7H 2 O (Wako); 0.005 g, MnSO 4 . H 2 O (Wako); 0.0016 g, ZnSO 4 · H 2 O (Wako); 0.0014 g, CoCl 2 ; (Wako) 0.0020 g, and distilled water: 1000 mL was used (pH 6.0) (9).…”

Factors Affecting the Genetic Stability of Non-parental Type Segregants Derived from Fusants betweenAspergillus oryzaeandAspergillus niger

“…In the fusants between A. oryzae and A. niger, the antibiotic resistance of A. niger line appeared to be more than that of the A. oryzae line although the antibiotic resistance of the A. oryzae line appeared to be more than that of the A. kawachii line in the fusants between A. oryzae and A. kawachii. 4 l It was suggested that this was caused by the difference of growth rate between parental strains, not by * To whom correspondence should be addressed. the multinuclear nature of A. oryzae.…”

Genetic Variants Derived from Autopolyploid Strains ofTrichoderma reeseiQM 9414

“…We have already reported that autopolyploid of T. reesei QM 9414 could be produced by colchicine treatment and that various changes were seen in conidial formation of sector strains by benomyl treatment of autopolyploid strains. 4 ) In this report, the genetic variants derived· from autopolyploid strains of T. reesei QM 9414 were investigated.…”