Autophagy-related circRNA evaluation reveals hsa_circ_0001747 as a potential favorable prognostic factor for biochemical recurrence in patients with prostate cancer

Abstract

Prostate cancer (PCa) is a common high-incidence malignancy in men, some of whom develop biochemical recurrence (BCR) in the advanced stage. However, there are currently no accurate prognostic indicators of BCR in PCa. The aim of our study was to identify an autophagy-related circular RNA prognostic factor of BCR for patients with PCa. In this study, immunochemistry revealed that the classic autophagy marker MAP1LC3B was positively correlated with Gleason score. Least absolute shrinkage and selector operator regression were conducted to develop a novel prognostic model with tenfold cross-validation and an L1 penalty. Five autophagy-related circRNA signatures were included in the prognostic model. Patients with PCa were ultimately divided into high- and low-risk groups, based on the median risk score. Patients with PCa, who had a high risk score, were more likely to develop BCR in a shorter period of time. Univariate and multivariate Cox regression analyses demonstrated that the risk score was an independent variable for predicting BCR in PCa. In addition, a prognostic nomogram integrated with the risk score and numerous clinicopathological parameters was developed to accurately predict 3- and 5-year BCR of patients with PCa. Finally, the hsa_circ_0001747 signature was selected for further experimental verification in vitro and in vivo, which showed that downregulated hsa_circ_0001747 might facilitate PCa via augmenting autophagy. Our findings indicate that the autophagy-related circRNA signature hsa_circ_0001747 may serve as a promising indicator for BCR prediction in patients with PCa.

5-Methylcytosine RNA Methyltransferases-Related Long Non-coding RNA to Develop and Validate Biochemical Recurrence Signature in Prostate Cancer

“…Finally, the two subgroups were then compared to validate the predictive model, too. We constructed the nomogram and calibration plots based on our previous study ( Zhong et al, 2021 ).…”

“…Transwell assay was conducted to examine the invasiveness of MAFG-AS1 downregulated cells. Detailed procedures of the above assays are available in our previous study ( Zhong et al, 2021 ). All experiments were implemented with three triplicates.…”

Autophagy-associated circRNA circATG7 facilitates autophagy and promotes pancreatic cancer progression

“…Therefore, clarifying the correlation of circRNAs and autophagy seems particularly important to the research about pancreatic cancer. In the study of autophagy-related circRNA, it has been found that there is an interactive regulatory relationship between circRNA and autophagy in non-small cell lung cancer [ 30 ], bladder cancer [ 31 ], and prostate cancer [ 32 ]. However, the regulatory relationship in pancreatic cancer is not clear.…”

The Role and Clinical Potentials of Circular RNAs in Prostate Cancer

“…The prognostic value of circRNAs has also assessed in PCa. For instance, expression levels of hsa_circ_0000437, hsa_circ_0000280, and circ_5017 have been correlated with poor survival of patients (97). Moreover, expression of circABCC4 has been associated with advanced clinicopathological features including higher tumor stage, metastasis and poor clinical outcomes (53).…”