Autophagy-mediated chemosensitization in cancer cells by fullerene C60 nanocrystal

Zhang, Qiang; Yang, Wenjun; Man, Na; Zheng, Fang; Shen, Yuanyuan; Sun, Kaixuan; Li, Yang; Wen, Longping

doi:10.4161/auto.5.8.9842

Cited by 168 publications

(150 citation statements)

References 39 publications

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“…24 In these cells, GFP-LC3 was distributed evenly in the cytosol, but upon transfection of the empty vector DNA with Lipofectamine 2000, a large number of green fluorescent dots were observed (Fig. 1A).…”

Section: Cationic Lipids Induced Lc3 Dot Formation and Conversionmentioning

confidence: 99%

Induction of genuine autophagy by cationic lipids in mammalian cells

Man¹,

Chen²,

Zheng³

et al. 2010

Autophagy

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Section: Cationic Lipids Induced Lc3 Dot Formation and Conversionmentioning

confidence: 99%

Induction of genuine autophagy by cationic lipids in mammalian cells

Man¹,

Chen²,

Zheng³

et al. 2010

Autophagy

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“…8,43 In respect to the autophagy induced by NPs, most of which can promote cell death. 44,45 This phenomenon has resulted in the viewpoint that the autophagy induced by NPs is essentially detrimental to cells, because NPs are likely to be incorporated into the autophagosomes during autophagy but cannot be degraded through the lysosomal pathway, which may disturb the normal autophagic process.…”

Section: Discussionmentioning

confidence: 99%

Iron oxide nanoparticles and induced autophagy in human monocytes

Jin²,

Feng

et al. 2017

IJN

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Superparamagnetic iron oxide nanoparticles have been widely used in biomedical applications, but understanding of their interactions with the biological immune system is relatively limited. This work is focused on dextran-coated iron oxide nanoparticles and their induced autophagy in human monocytes. We found that these nanoparticles can be taken up by human monocytes, followed by localization within vesicles or free in cytoplasm. Autophagosome formation was observed with increased expression of LC3II protein, the specific marker of autophagy. The autophagy substrate p62 was degraded in a dose-dependent manner, and autophagy was blocked by autophagy (or lysosome) inhibitors alone or along with iron oxide nanoparticles, indicating that autophagosome accumulation was mainly due to autophagy induction, rather than blockade of autophagy flux. Interestingly, iron oxide nanoparticles increased the viability of human monocytes, but the mechanism was not clear. We further found that inhibition of autophagy mostly attenuated the survival of cells, with acceleration of the inflammation induced by these nanoparticles. Taken together, autophagic activation in human monocytes may play a protective role against the cytotoxicity of iron oxide nanoparticles.

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“…Recently, various kinds of nanomaterials were reported to be able to induce autophgay [17,[22][23][24][25]. Based on the different mode of intracellular substrates entering lysosome, there are three forms of autophagy: macroautophagy, microautophagy and chaperone-mediated autophagy.…”

Section: Autophagymentioning

confidence: 99%

“…Hence, the authors proposed that autophagy might be involved in the anti-proliferative funtion of nC 60 . Zhang et al [38] systematically studied the effect of water-dispersed nanocrystal C 60 and its derivative C 60 (Nd) nanoparticles on autophagy. Their results indicated that nC 60 could induce autophagy and sensitize chemotherapeutic killing of both normal and drug-resistant cancer cells at noncytotoxic concentrations (0.5 μg/mL).…”

Section: And C 60 Derivativesmentioning

confidence: 99%

“…Autophagy can crosstalk with other cellular process, such as apoptosis and necrosis, and therefore is an important part of the cellular effect of nanomaterials [15,16]. Moreover, nanomaterials-induced autophagy showed potential for the treatment of certain diseases including neurodegenerative diseases and cancer [13,17]. Hence, in this review we summarize the current research on the effect of various kinds of nanomaterials on autophagy.…”

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Autophagy as new emerging cellular effect of nanomaterials

et al. 2013

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The biosafety issue of nanoscale materials is getting more and more attention with their increasing manufacture and application. In the research of cellular effects and underlying mechanisms related to toxicity of nanomaterials, most emphasis were placed on processes such as apoptosis, metabolic inhibition and oxidative stress. Recent evidence suggests that autophagy is part of the biological effects by nanomaterials and various kinds of nanomaterials are capable of disturbing the autophagic process. This review will highlight the importance of autophagy as an emerging mechanism of nanomaterial toxicity and the implication in the therapy of autophagy-related diseases. We summarize current research status of interaction between nanomaterials and autophagic pathways. It is of note that nanomaterials can either induce or block autophagy, which result in similar phenotype but completely different biological consequence. It is therefore important to perform comprehensive analysis of the whole autophagic flux in the future research. Nanomaterials refer to materials with the size range between 1 and 100 nanometers in one or more dimensions [1]. Due to their superior physicochemical properties, such as ultra small size, increased ratio of surface area to volume, multiple options for modification and relatively good biocompatibilities, application of nanomaterials in research as well as in industry becomes more and more common. It is predicted that the total amount of nanomaterials' production will increase up to 58000 tons by 2020. Particularly, the utilization of nanomaterials in biomedical research as biosensors, drug-carriers, or imaging agents was extensively studied [2][3][4]. Large scaled preparation and application of nanomaterials increase their possibilities to be exposed to human beings or enter the environment [5][6][7]. Therefore the impact of nanomaterials on environment and human health has attracted high attention. Related studies were carried out on the biological effect of various kinds of nanomaterials both in vitro and in vivo [8]. To date, reported physiological changes of a cell or an organism that exposed to nanomaterials include: reactive oxygen species (ROS)-related pathological responses (such as damaged membrane, mitochondrial damage and necrosis), apoptosis, inflammation and altered differentiation patterns, etc [9][10][11][12]. Recently, autophagy as an essential cellular process and a novel biological effect of nanomaterials on eukaryotes, has received much attention. Many nanomaterials were reported to be able to change the basal level of autophagy [13,14]. Autophagy can crosstalk with other cellular process, such as apoptosis and necrosis, and therefore is an important part of the cellular effect of nanomaterials [15,16]. Moreover, nanomaterials-induced autophagy showed potential for the treatment of certain diseases including neurodegenerative diseases and cancer [13,17]. Hence, in this review we summarize the current research on the effect of various kinds of nanomaterials on autophagy. ...

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Autophagy-mediated chemosensitization in cancer cells by fullerene C60 nanocrystal

Cited by 168 publications

References 39 publications

Induction of genuine autophagy by cationic lipids in mammalian cells

Induction of genuine autophagy by cationic lipids in mammalian cells

Iron oxide nanoparticles and induced autophagy in human monocytes

Autophagy as new emerging cellular effect of nanomaterials

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