Autophagy Inhibition Sensitizes Renal Tubular Epithelial Cell to G1 Arrest Induced by Transforming Growth Factor beta (TGF-β)

Yang, Chen; Wu, Huixing; Li, Zhi-Hang; Chen, Jiayao; Su, Hong-yong; Guo, Xiaoyan; Jing, Kaipeng; Liu, Huafeng

doi:10.12659/msm.922673

Cited by 5 publications

(2 citation statements)

References 54 publications

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“…The cell cycle assay was performed using Cell Cycle and Apoptosis Analysis Kit (Beyotime Biotechnology, C1052) as described previously 24 . Briefly, HK‐2 cells were harvested and fixed in cold 70% ethanol at 4°C overnight.…”

Section: Methodsmentioning

confidence: 99%

Cyclosporine A blocks autophagic flux in tubular epithelial cells by impairing TFEB‐mediated lysosomal function

Huang

Yang

et al. 2021

J Cellular Molecular Medi

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Cyclosporine A (CsA) is an immunosuppressor widely used for the prevention of acute rejection during solid organ transplantation. However, severe nephrotoxicity has substantially limited its long‐term usage. Recently, an impaired autophagy pathway was suggested to be involved in the pathogenesis of chronic CsA nephrotoxicity. However, the underlying mechanisms of CsA‐induced autophagy blockade in tubular cells remain unclear. In the present study, we observed that CsA suppressed the activation and expression of transcription factor EB (TFEB) by increasing the activation of mTOR, in turn promoting lysosomal dysfunction and autophagy flux blockade in tubular epithelial cells (TECs) in vivo and in vitro. Restoration of TFEB activation by Torin1‐mediated mTOR inhibition significantly improved lysosomal function and rescued autophagy pathway activity, suppressing TEC injury. In summary, targeting TFEB‐mediated autophagy flux represents a potential therapeutic strategy for CsA‐induced nephrotoxicity.

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Section: Methodsmentioning

confidence: 99%

Cyclosporine A blocks autophagic flux in tubular epithelial cells by impairing TFEB‐mediated lysosomal function

Huang

Yang

et al. 2021

J Cellular Molecular Medi

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“…They also reported that tubular cells with autophagosome accumulation are little proliferative with delayed tubular repair [73]. Moreover, blockage of autophagic flux by the lysosome inhibitor, chloroquine or bafilomycin A, promotes G1-phase cell cycle arrest in- duced by transforming growth factor-β in renal TECs [74]. Thus, impaired autophagic clearance and lysosome depletion may promote tubular injury and delay tubular repair.…”

Section: Acute Kidney Injurymentioning

confidence: 99%

Lysosome Depletion-Triggered Autophagy Impairment in Progressive Kidney Injury

Chen

Yang

et al. 2021

Kidney Dis

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Background: Macroautophagy (autophagy) is a cellular recycling process involving the destruction of damaged organelles and proteins in intracellular lysosomes for efficient nutrient reuse. Summary: Impairment of the autophagy-lysosome pathway is tightly associated with multiple kidney diseases, such as diabetic nephropathy, proteinuric kidney disease, acute kidney injury, crystalline nephropathy, and drug- and heavy metal-induced renal injury. The impairment in the process of autophagic clearance may induce injury in renal intrinsic cells by activating the inflammasome, inducing cell cycle arrest, and cell death. The lysosome depletion may be a key mechanism triggering this process. In this review, we discuss this pathway and summarize the protective mechanisms for restoration of lysosome function and autophagic flux via the endosomal sorting complex required for transport (ESCRT) machinery, lysophagy, and transcription factor EB-mediated lysosome biogenesis. Key Message: Further exploring mechanisms of ESCRT, lysophagy, and lysosome biogenesis may provide novel therapy strategies for the management of kidney diseases.

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Jiedu Tongluo Baoshen formula enhances renal tubular epithelial cell autophagy to prevent renal fibrosis by activating SIRT1/LKB1/AMPK pathway

Jin¹,

Zhao²,

Sun³

et al. 2023

Biomedicine & Pharmacotherapy

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Autophagy Inhibition Sensitizes Renal Tubular Epithelial Cell to G1 Arrest Induced by Transforming Growth Factor beta (TGF-β)

Cited by 5 publications

References 54 publications

Cyclosporine A blocks autophagic flux in tubular epithelial cells by impairing TFEB‐mediated lysosomal function

Cyclosporine A blocks autophagic flux in tubular epithelial cells by impairing TFEB‐mediated lysosomal function

Lysosome Depletion-Triggered Autophagy Impairment in Progressive Kidney Injury

Jiedu Tongluo Baoshen formula enhances renal tubular epithelial cell autophagy to prevent renal fibrosis by activating SIRT1/LKB1/AMPK pathway

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