Autophagy induction reduces telomerase activity in HeLa cells

Taji, Fatemeh; Kouchesfahani, Homa Mohseni; Sheikholeslami, Farzaneh; Romani, Bizhan; Baesi, Kazem; Vahabpour, Rouhollah; Edalati, Mahdi; Teimoori‐Toolabi, Ladan; Jazaeri, Ehsan Ollah; Abdoli, Asghar

doi:10.1016/j.mad.2016.12.011

Cited by 23 publications

(16 citation statements)

References 38 publications

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“…Injury- and amino acid restriction-induced autophagy can be suppressed by depletion of telomerase and we show that telomerase activates autophagy in the glucose-starvation condition [ 9 , 42 ]. Alternatively, Beclin 1-mediated autophagy reduces telomerase activity suggesting that telomerase and autophagy are related vice versa [ 43 ]. The evidences imply that telomerase protects the cells from stresses by activating autophagy and telomerase itself can be suppressed by autophagy in a mean of negative feedback.…”

Section: Discussionmentioning

confidence: 99%

Hexokinase 2 is a molecular bridge linking telomerase and autophagy

Roh

Kim

et al. 2018

PLoS ONE

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Autophagy is systematically regulated by upstream factors and nutrients. Recent studies reported that telomerase and hexokinase 2 [HK2) regulate autophagy through mTOR and that telomerase has the capacity to bind to the HK2 promoter. However, the molecular linkage among telomerase, HK2, and autophagy is not fully understood. Here, we show that HK2 connects telomerase to autophagy. HK2 inhibition in HepG2 cells suppressed TERT-induced autophagy activation and further enhancement by glucose deprivation. The HK2 downstream factor mTOR was responsible for the TERT-induced autophagy activation under glucose deprivation, implying that TERT promotes autophagy through an HK2-mTOR pathway. TERC played a role similar to that of TERT, and simultaneous expression of TERT and TERC synergistically enhanced HK2 expression and autophagy. At the gene level, TERT bound to the HK2 promoter at a specific region harboring the telomerase-responsive sequence ‘.’ Mutagenesis of TERC and the TERT-responsive element in the HK2 promoter revealed that TERC is required for the binding of TERT to the HK2 promoter. We demonstrate the existence of a telomerase-HK2-mTOR-autophagy axis and suggest that inhibition of the interaction between telomerase and the HK2 promoter diminishes glucose starvation-induced autophagy.

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Section: Discussionmentioning

confidence: 99%

Hexokinase 2 is a molecular bridge linking telomerase and autophagy

Roh

Kim

et al. 2018

PLoS ONE

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“…It is well documented that telomerase activation by viral oncoproteins such as HBV X protein (HBx) and HCV core is connected to carcinoma. The results of our recent study suggest that the induction of autophagy reduces telomerase activity in tumor cells . Thus, pharmacological induction of autophagy may reduce telomerase activity and tumor progression.…”

Section: Perspectivementioning

confidence: 78%

Autophagy: The multi‐purpose bridge in viral infections and host cells

Abdoli

Alirezaei

Mehrbod

et al. 2018

Reviews in Medical Virology

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Autophagy signaling pathway is involved in cellular homeostasis, developmental processes, cellular stress responses, and immune pathways. The aim of this review is to summarize the relationship between autophagy and viruses. It is not possible to be fully comprehensive, or to provide a complete "overview of all viruses". In this review, we will focus on the interaction of autophagy and viruses and survey how human viruses exploit multiple steps in the autophagy pathway to help viral propagation and escape immune response. We discuss the role that macroautophagy plays in cells infected with hepatitis C virus, hepatitis B virus, rotavirus gastroenteritis, immune cells infected with human immunodeficiency virus, and viral respiratory tract infections both influenza virus and coronavirus.

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“…1d). It has been reported that cell telomerase activity highly relevant to cell cycle regulation, and the highest levels of cell telomerase activity occur in the S phase [18,19]. In fact, in vivo bone development is a process in which the pluripotency and proliferative ability of cells decreases gradually [20].…”

Section: Cell Cycle Changes During the Osteogenic Differentiation Of mentioning

confidence: 99%

Establishing a deeper understanding of the osteogenic differentiation of monolayer cultured human pluripotent stem cells using novel and detailed analyses

Zhou

Shi

Song³

et al. 2020

Preprint

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Background: Derivation of osteoblast-like cells from human pluripotent stem cells (hPSCs) is a popular topic in bone tissue engineering. Although many improvements have been achieved, the low induction efficiency because of spontaneous differentiation hampers their applications. To solve this problem, a detailed understanding of the osteogenic differentiation process of hPSCs is urgently needed.Methods: Monolayer cultured human embryonic stem cells and human induced pluripotent stem cells were differentiated in commonly applied serum-containing osteogenic medium for 35 days. In addition to traditional assays such as cell viability detection, reverse transcription-polymerase chain reaction, immunofluorescence, and Alizarin red staining, we also applied studies of cell counting, cell telomerase activity, and flow cytometry as essential indicators to analyse the cell type changes in each week. Results: The population of differentiated cells was quite heterogeneous throughout the 35 days of induction. Then, cell telomerase activity and cell cycle analyses have value in evaluating the cell type and tumourigenicity of the obtained cells. Finally, a dynamic map was made to integrated the analysis of these results during osteogenic differentiation of hPSCs, and the cell types at defined stages were concluded. Conclusions: Our results lay the foundation to improve the in vitro osteogenic differentiation efficiency of hPSCs by supplementing with functional compounds at the desired stage, and then establishing a step-wise induction system in the future.

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Autophagy induction reduces telomerase activity in HeLa cells

Cited by 23 publications

References 38 publications

Hexokinase 2 is a molecular bridge linking telomerase and autophagy

Hexokinase 2 is a molecular bridge linking telomerase and autophagy

Autophagy: The multi‐purpose bridge in viral infections and host cells

Establishing a deeper understanding of the osteogenic differentiation of monolayer cultured human pluripotent stem cells using novel and detailed analyses

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