Autophagy genes AMBRA1 and ATG8 play key roles in midgut remodeling of the yellow fever mosquito, Aedes aegypti

Albishi, Najla M.; Palli, Subba Reddy

doi:10.3389/finsc.2023.1113871

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…In the mosquito, Anopheles gambiae, larvae, chitosan and dsRNA formulations improved RNAi efficiency (196). Several other nanoformulations of dsRNA, including guanidine-containing polymers, nanocarrier/dsRNA/detergent formulation, guanylated polymers and branched amphiphilic peptide bilayer conjugated gold nanoparticles were also shown to improve RNAi (175,(197)(198)(199)(200)(201)(202)(203)(204)(205)(206)(207)(208)(209)(210)(211)(212)(213)(214)(215). The nanoformulations of dsRNA help protect dsRNA from dsRNases (210)(211)(212)(213)(214), facilitate dsRNA penetration through the The major roadblocks to efficient RNAi in insects.…”

Section: Strategies To Improve Rnai Efficiency In Major Pests and Dis...mentioning

confidence: 99%

RNAi turns 25:contributions and challenges in insect science

Palli

2023

Front. Insect Sci.

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Since its discovery in 1998, RNA interference (RNAi), a Nobel prize-winning technology, made significant contributions to advances in biology because of its ability to mediate the knockdown of specific target genes. RNAi applications in medicine and agriculture have been explored with mixed success. The past 25 years of research on RNAi resulted in advances in our understanding of the mechanisms of its action, target specificity, and differential efficiency among animals and plants. RNAi played a major role in advances in insect biology. Did RNAi technology fully meet insect pest and disease vector management expectations? This review will discuss recent advances in the mechanisms of RNAi and its contributions to insect science. The remaining challenges, including delivery to the target site, differential efficiency, potential resistance development and possible solutions for the widespread use of this technology in insect management.

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Section: Strategies To Improve Rnai Efficiency In Major Pests and Dis...mentioning

confidence: 99%

RNAi turns 25:contributions and challenges in insect science

Palli

2023

Front. Insect Sci.

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“…Autophagy is primarily determined by autophagy-related (ATG) proteins. Juvenile hormone (JH) can inhibit the expression of ATG proteins, thereby suppressing autophagy during metamorphic development and influencing midgut remodeling during this stage [18]. Through a genome-wide analysis of apoptosis-related genes in the silkworm, three caspases were identified, namely, BmCaspase-N, BmCaspase-1, and BmICE [19].…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of the Midgut Contents of Silkworm in the Pupal Stage

Wang,

Yang,

Tian

et al. 2023

Insects

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The silkworm Bombyx mori, a lepidopteran insect, possesses an 8–10-day pupal stage, during which significant changes occur in the midgut, where it first condenses into the yellow body, and then undergoes decomposition. To gain insights into this transformation process, proteomics was performed on Bombyx mori midgut contents on day 2 and day 7 after pupation. The results revealed the identification of 771 proteins with more than one unique peptide. An analysis using AgriGO demonstrated that these proteins were predominantly associated with catalytic activity. Among the identified proteins, a considerable number were found to be involved in carbohydrate metabolism, amino acid metabolism, lipid metabolism, nucleic acid degradation, and energy support. Additionally, variations in the levels of certain proteases were observed between the midgut contents on day 2 and day 7 after pupation. An in-depth analysis of the two-dimensional electrophoresis of the midgut contents on day 7 after pupation led to the identification of twelve protein spots with potential gelatinolytic activity. Among these, six proteases were identified through mass spectrometry, including the p37k protease, vitellin-degrading protease, chymotrypsin-2, etc. These proteases may be responsible for the digestion of the yellow body during the later stages of pupal development.

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Autophagy genes AMBRA1 and ATG8 play key roles in midgut remodeling of the yellow fever mosquito, Aedes aegypti

Cited by 2 publications

References 33 publications

RNAi turns 25:contributions and challenges in insect science

RNAi turns 25:contributions and challenges in insect science

Proteomic Analysis of the Midgut Contents of Silkworm in the Pupal Stage

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