Autophagy-Associated lncRNAs: Promising Targets for Neurological Disease Diagnosis and Therapy

Xu, Xiangming; Cui, Lili; Zhong, Wangtao; Cai, Yujie

doi:10.1155/2020/8881687

Cited by 27 publications

(17 citation statements)

References 106 publications

(107 reference statements)

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“…miR-19a and miR-19b representative validated target genes of miR-19a and miR-19b are shown in Table 3A–C . Phosphatase and Tensin Homolog (PTEN), 21 , 22 Rap Guanine Nucleotide Exchange Factor (2Rapgef2), 23 voltage-gated potassium channel subunits, 20 Tumor Protein p53 (p53), 24 and RUNX Family Transcription Factor 3 (RUNX3) 25 are common targets shared by both miR-19a and miR-19b. Further, miR-19a has its own validated targets including Suppressor of Cytokine Signaling 1 (SOCS1), Methyl-CpG Binding Protein 2 (MeCP2), Ras Homolog Family Member B (RhoB), Peroxisome Proliferator Activated Receptor Alpha (PPARα), and Leucine Rich Repeats and Immunoglobulin Like Domains 1 (LRIG1).…”

Section: Resultsmentioning

confidence: 99%

hsa-MiR-19a-3p and hsa-MiR-19b-3p Are Associated with Spinal Cord Injury-Induced Neuropathic Pain: Findings from a Genome-Wide MicroRNA Expression Profiling Screen

Liang

Morse

Falci

et al. 2021

Neurotrauma Reports

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Neuropathic pain in spinal cord injury (SCI) is associated with inflammation in both the peripheral and central nervous system (CNS), which may contribute to the initiation and maintenance of persistent pain. An understanding of factors contributing to neuroinflammation may lead to new therapeutic targets for neuropathic pain. Moreover, novel circulating biomarkers of neuropathic pain may facilitate earlier and more effective treatment. MicroRNAs (miRNAs) are short, non-coding single-stranded RNA that have emerged as important biomarkers and molecular mediators in physiological and pathological conditions. Using a genome-wide miRNA screening approach, we studied differential miRNA expression in plasma from 68 healthy, community-dwelling adults with and without SCI enrolled in ongoing clinical studies. We detected 2367 distinct miRNAs. Of these, 383 miRNAs were differentially expressed in acute SCI or chronic SCI versus no SCI and 71 were differentially expressed in chronic neuropathic pain versus no neuropathic pain. We selected homo sapiens (hsa)-miR-19a-3p and hsa-miR-19b-3p for additional analysis based on p -value, fold change, and their known role as regulators of neuropathic pain and neuroinflammation. Both hsa-miR-19a-3p and hsa-miR-19b-3p levels were significantly higher in those with chronic SCI and severe neuropathic pain versus those with chronic SCI and no neuropathic pain. In confirmatory studies, both hsa-miR-19a-3p and hsa-miR-19b-3p have moderate to strong discriminative ability to distinguish between those with and without pain. After adjusting for opioid use, hsa-miR-19b-3p levels were positively associated with pain interference with mood. Because hsa-miR-19 levels have been shown to change in response to exercise, folic acid, and resveratrol, these studies suggest that miRNAs are potential targets of therapeutic interventions.

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Section: Resultsmentioning

confidence: 99%

hsa-MiR-19a-3p and hsa-MiR-19b-3p Are Associated with Spinal Cord Injury-Induced Neuropathic Pain: Findings from a Genome-Wide MicroRNA Expression Profiling Screen

Liang

Morse

Falci

et al. 2021

Neurotrauma Reports

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“…LncRNAs are highly expressed in mammalian brains and are linked with brain development and function [13]. Recent studies have found that lncRNA is abnormally expressed in PD patients, suggesting that lncRNA may serve the purpose of regulating the onset and advancing of PD [14].…”

Section: Discussionmentioning

confidence: 99%

LncRNA RMST Regulates Neuronal Apoptosis and Inflammatory Response via Sponging miR-150-5p in Parkinson’s Disease

Chuanlei

Zhang

Wei

et al. 2021

Neuroimmunomodulation

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Introduction: LncRNA rhabdomyosarcoma 2-associated transcript (RMST) serves as a key regulator in neural stem cell fate and is involved in the progression of different neurological diseases. In this research, the serum level and clinical value of RMST in Parkinson’s disease (PD) patients were detected, and the underlying mechanism was explored. Methods: Ninety-nine PD patients and 93 healthy individuals were collected for clinical experiments. SH-SY5Y cells were treated with the dopaminergic neurotoxin 1-methyl-4-phenylpyridinium (MPP+) to establish PD cell models. qRT-PCR was used for the detection of mRNA levels. CCK-8 and flow cytometry were used to detect neuronal viability and apoptosis. The target relationship of RMST with miR-15a-5p was confirmed applying luciferase reporter assay. Results: RMST was present at high levels in both serum of PD patients and PD cell models. Serum RMST had a certain clinical value for the diagnosis of PD with the AUC of 0.892 at a cutoff value of 1.225. Serum RMST was positively associated with the levels of TNF-α (r = 0.421, p < 0.001) and IL-1β (r = 0.567, p < 0.001) in PD patients. Knockdown of RMST alleviated the apoptosis and inflammatory response of SH-SY5Y cells induced by MPP+. miR-150-5p was the target gene of RMST and less expressed in the clinical serum samples and PD cell models. Conclusion: Serum RMST serves as a promising biomarker for the diagnosis of PD. RMST downregulation may regulate the occurrence and development of PD through inhibiting neuron cell apoptosis and the release of inflammatory cytokines via targeting miR-150-5p.

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“…Long non-coding RNAs (lncRNAs) are transcripts with a length of more than 200 nucleotides without protein coding function. LncRNAs have involved in biological processes as an important role, such as proliferation, differentiation, apoptosis, invasion, and metastasis of cancer [13,14].Recently, growing evidence indicated that lncRNAs primarily participant in the occurrence and development of cancer, which may related to its potential of regulating autophagy [15,16].Thus, developing prognostic biomarker related to autophagy for PC patients may be helpful in treatment and prognosis of PC.…”

Section: Introductionmentioning

confidence: 99%

An Independent Prognostic Model Based on Ten Autophagy-Related Genes in Pancreatic Cancer

Tian

Zhang

et al. 2021

Preprint

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Purpose Pancreatic cancer(PC) is a common cancer with high lethality and low survival rate. Autophagy is involved in the biological process of PC. Thus, we intended to explore the function of autophagy-related long noncoding RNA signature for survival assessment in PC. Methods Based on 10 autophagy-related lncRNAs, the prognostic model was attained through univariate and multivariate Cox regression analysis. Subsequently, the relationship network of 10 lncRNAs was crystallized in co-expression network and Sankey diagram. Survival analysis and ROC curve were used to evaluate the signature. GSEA was utilized to screen enriched gene sets. Result The OS has significant deference in low-risk group and high-risk group(P < 0.001). The ROC curve further proved the potential utility of the signature(AUC = 0.815). GSEA was significantly enriched in cancer-related gene sets. Conclusion The signature has potential to evaluate clinical prognosis in PC. The 10 autophagy-related lncRNAs may achieve great development for PC in target therapy field.

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Autophagy-Associated lncRNAs: Promising Targets for Neurological Disease Diagnosis and Therapy

Cited by 27 publications

References 106 publications

hsa-MiR-19a-3p and hsa-MiR-19b-3p Are Associated with Spinal Cord Injury-Induced Neuropathic Pain: Findings from a Genome-Wide MicroRNA Expression Profiling Screen

hsa-MiR-19a-3p and hsa-MiR-19b-3p Are Associated with Spinal Cord Injury-Induced Neuropathic Pain: Findings from a Genome-Wide MicroRNA Expression Profiling Screen

LncRNA RMST Regulates Neuronal Apoptosis and Inflammatory Response via Sponging miR-150-5p in Parkinson’s Disease

An Independent Prognostic Model Based on Ten Autophagy-Related Genes in Pancreatic Cancer

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