Autonomy of growth and of iodine metabolism in hyperthyroid feline goiters transplanted onto nude mice.

Abstract

Hyperthyroidism caused by nodular goiters is a common disease of aging cats. Growth and iodine metabolism were studied by autoradiography in normal and hyperfunctioning thyroid tissue obtained from cats injected with 125I before surgery, and in xenografts, grown in nude mice, after double-labeling with 131I and [Hjthymidine.Hyperthyroid cat goiters contain single or multiple hyperplastic nodules, consisting of highly cellular tissue with an iodine metabolism exceeding that of the surrounding normal tissue.

American Thyroid Association Guide to Investigating Thyroid Hormone Economy and Action in Rodent and Cell Models

“…Autoradiography experiments with human, rodent, and feline goiter tissue have also been performed after xenotransplantation of thyroid tissue into nude mice. Subcutaneously implanted fragments are maintained in recipient mice for several weeks before further analysis (19). & …”

Follicle-forming cat thyroid cell lines synthesizing extracellular matrix and basal membrane components: a new tool for the study of thyroidal morphogenesis

“…In the present study we report the investigation of ECM synthesis by different feline thyroid cell lines (Romcat, Petcat) (Peter et al 1987, 1991a-c, Gerber et al 1991a,b, 1994 and v-ras-transfected sublines. For transfection, we have introduced the pZSR plasmid harboring the viral Harvey ras gene or the pSV2-neo plasmid only (as control) into the Romcat cell line (Gerber et al 1991a(Gerber et al ,b, 1994.…”

“…Thyroid tissue of hyperthyroid cats (Peter et al 1987, Gerber et al 1994) was obtained from Dr M E Peterson, Cornell University, New York, NY, USA. Different cell lines have been established from clinically wellcharacterized toxic nodular cat goiters and characterized as described (Peter et al 1987, 1991a-c, Gerber et al 1991a,b, 1994 (Table 1).…”

“…Different cell lines have been established from clinically wellcharacterized toxic nodular cat goiters and characterized as described (Peter et al 1987, 1991a-c, Gerber et al 1991a,b, 1994 (Table 1). In this study, we mainly used the Romcat cell line and eight v-ras-transfected sublines as well as two sublines transfected only with pSV2-neo (see below) ( Table 1).…”

“…In order to study in vitro thyroid differentiation, function and proliferation, various cell culture systems have been tested: monolayers (Ambesi-Impiombato et al 1980, Ambesi-Impiombato & Perrild 1989, Derwahl et al 1990, Gerber et al 1991a,b, Peter et al 1991b, Dumont et al 1992, three-dimensional systems using collagen gels (Yang et al 1979, Garbi & Wollman 1982, Garbi et al 1984, Peter et al 1987, 1991a-c, Toda & Sugihara 1990, Toda et al 1993, agarose gels (Kimura et al 1980, Benya & Shaffer 1982, Aydelotte et al 1986a,b, Bruckner et al 1989) and alginate beads (Kupchick et al 1983, Guo et al 1989, Häuselmann et al 1994, Tognella et al 1995, Glaser et al 1996, Bürgi-Saville et al 1997, 1998. Alginate has several advantages in comparison with the other systems.…”