Autonomic innervation of the rat vagina, cervix, and uterus and its cyclic variation

Adham, Nabila; Schenk, Eric A.

doi:10.1016/s0002-9378(16)34239-9

Cited by 115 publications

(65 citation statements)

References 13 publications

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“…This suggestion is supported by the histochemical demonstration of dense acetylcholinesterase-staining in the present study and by other workers (Adham & Schenk, 1969;Hervonen et aL, 1973). Nakanishi & Wood (1971) by atropine.…”

Section: Discussionsupporting

confidence: 92%

THE INNERVATION OF THE RATCERVIX AND ITS PHARMACOLOGY In vitro AND In vivo

Hollingsworth

1974

British J Pharmacology

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1Contractions of the rat cervix and uterine horns were measured both in vitro and in vivo as changes in luminal perfusion pressure during perfusion at constant flow. 2 Spontaneous cervical and uterine horn contractions in vitro were reduced from rats pre-treated with 1701-oestradiol compared to those ovariectomized only or those pre-treated with 1703-oestradiol plus progesterone.3 The rat cervix exhibited similar in vitro sensitivities to methacholine, oxytocin, isoprenaline and phenylephrine as did the uterine horns. 4 Transmural stimulation produced cervical and uterine horn contractions, which were blocked by hyoscine or tetrodotoxin, suggesting a cholinergic motor innervation. This was supported by histological findings. 5In vivo, the injections of propranolol (1.9 x 10-7mol/kg) or pempidine (3.2 or 6.4 x 10-6 mol/kg) were followed by large cervical and smaller uterine horn contractions, suggesting an adrenergic inhibitory tone mediated by 3-adrenoceptors. 6 The evidence for a cervical sphincter is discussed.

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Section: Discussionsupporting

confidence: 92%

THE INNERVATION OF THE RATCERVIX AND ITS PHARMACOLOGY In vitro AND In vivo

Hollingsworth

1974

British J Pharmacology

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“…Decreased gastrointestinal motility and constipation are well known consequences of treatment with atropine (lIenderson & Roepke, 1937;Ambache, 1955) and ganglion blocking agents (Paton & Zaimis, 1951). Cholinesterase inhibition, on the other hand, increases gut motility due to the accumulation of acetylcholine (Holmstedt, 1959) (Adham & Schenk, 1969;Silva, 1966). This would go along with the low level of acetylcholinesterase activity which was found in the uterus in the present study.…”

Section: Discussionsupporting

confidence: 53%

Response of the rat ileum, uterus and vas deferens to carbachol and acetylcholine following repeated daily administration of a cholinesterase inhibitor

Foley

McPhillips

1973

British J Pharmacology

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Summary Daily i.p. administration, for eight days, of the cholinesterase inhibitor disulfoton to rats produced mild to moderate signs of intoxication (tremors, incontinence and diarrhoea) but no deaths. Segments of ileum taken from the treated rats were subsensitive to carbachol but the vas deferens and the uterus did not exhibit any change in sensitivity to carbachol. The sensitivity to acetylcholine was increased in the ileum and vas deferens but not in the uterus. Acetylcholinesterase activity was 60–70% inhibited in all three tissues.

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“…Hormonal changes may in¯uence the innervation of vagina. Adham and Schenk showed changes in density of adrenergic and cholinergic innervation during the oestrous cycle 44 and the NOS expression has been shown to be hormonally modulated. 45 ± 47 Given the difference between cycles of our control and DM rats one could speculate that the differences between the two groups are due to hormonal changes and not due to the diabetes.…”

Section: Discussionmentioning

confidence: 99%

Effects of diabetes on neurotransmission in rat vaginal smooth muscle

et al. 2001

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The aim of this work was to characterize the effect of experimental diabetes on neurotransmission in rat vagina. Female Sprague ± Dawley rats were divided into two groups: non-diabetic controls (NDM, n 38) and diabetics (DM, n 38). DM was produced by intraperitoneal injection of streptozotocin. Eight weeks later the animals were killed, the distal part of the vagina was removed, and smooth muscle strips were prepared for functional organ bath experiments and for measurement of nitric oxide synthase (NOS) activity. In DM preparations, the EC 50 value for noradrenaline (NA) was signi®cantly increased (P`0.05) and the maximal contractile response decreased (P 5 0.001). In preparations precontracted with NA, the NO donor SNAP and calcitonin gene-related peptide (CGRP) caused concentration-dependent relaxations, which were signi®cantly decreased (P`0.001) in the DM group. Electrical stimulation of nerves (EFS) caused frequency-dependent contractions, which were signi®cantly lower in DM than in NDM strips (P`0.001). SNAP and CGRP concentration-dependently inhibited EFS evoked contractions in both NDM and DM preparations. The inhibition was signi®cantly lower (P`0.05) in the DM group. In NDM preparations precontracted with NA, EFS evoked frequency-dependent relaxations; such relaxations were inhibited or reduced in DM. Treatment with the NOS inhibitor, L-NOARG 0.1 mM, abolished relaxations in all preparations or produced contraction in DM preparations. Calcium-dependent NOS activity was not signi®cantly different in the DM and NDM groups. However, the DM animals showed a small but signi®cant increase in calcium-independent NOS-activity (P`0.05). Diabetes interferes with adrenergic-, cholinergic-and NANC-neurotransmitter mechanisms in the smooth muscle of the rat vagina. The changes in the nitrergic neurotransmission are not due to reduction in NOS-activity, but seem to be due to interference with later steps in the L-arginineaNOaguanylate cyclaseacGMP system.

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Autonomic innervation of the rat vagina, cervix, and uterus and its cyclic variation

Cited by 115 publications

References 13 publications

THE INNERVATION OF THE RATCERVIX AND ITS PHARMACOLOGY In vitro AND In vivo

THE INNERVATION OF THE RATCERVIX AND ITS PHARMACOLOGY In vitro AND In vivo

Response of the rat ileum, uterus and vas deferens to carbachol and acetylcholine following repeated daily administration of a cholinesterase inhibitor

Effects of diabetes on neurotransmission in rat vaginal smooth muscle

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