Automation of the glycerolization of red blood cells with the high‐separation bowl in the Haemonetics ACP 215 instrument

Valeri, C. R.; Ragno, Gina; Houten, Paul Van; Rose, Leslie; Rose, Michael; Egozy, Yair; Popovsky, Mark

doi:10.1111/j.1537-2995.2005.00588.x

Cited by 43 publications

(22 citation statements)

References 13 publications

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“…The scenario has changed with introduction of glycerolization and deglycerolization by NBRL method with a significant reduction in processing time [2,3]. Further, deglycerolized RBC prepared by NBRL method has a shelf life of 14 days, resulting in reduction of wastage rate and cost per unit of blood [8].…”

Section: Discussionmentioning

confidence: 99%

“…High cost, difficulty in preparation and short shelf life of thawed FS-RBC were deterrents that dampened the enthusiasm for FS-RBC usages [1]. Advancement in technology during last few years wit the introduction of ACP TM 215 Haemonetics cell processing system (ACP 215) in preparation of FS-RBC has taken care of most of deterrents of FS-RBC [2,3]. In this background, there is need to re-look at FS-RBC as part of Frozen Blood Programme for Indian Armed Forces.…”

Section: N 1950mentioning

confidence: 99%

“…ACP 215 uses disposable glycerolization or deglycerolization kits. The machine is easy to operate for both protocols [2,3]. The sterile connecting device (SCD) is used for attaching additional bags and compatible tubing to a blood bag without breaking sterile integrity of the system [4].…”

Section: Equipmentsmentioning

confidence: 99%

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Frozen Red Blood Cells in Transfusion

Chaudhari

2009

Medical Journal Armed Forces India

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Section: Discussionmentioning

confidence: 99%

Section: N 1950mentioning

confidence: 99%

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Frozen Red Blood Cells in Transfusion

Chaudhari

2009

Medical Journal Armed Forces India

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“…The major event to simplify the washing of human RBC treatedwith 40% W/V glycerol was the introduction in 1998 by Haemonetics Corporation, Braintree, MA of the Automated Cell Processor (ACP) 215 which is a functionally closed instrument to glycerolize and deglycerolize the red blood cells with storage in the additive solution AS-3 (Nutricel) at 4°C for 2 weeks which is an FDA approved procedure [13][14][15].…”

mentioning

confidence: 99%

“…Automation of RBC glycerolization using the high separation bowl attached to the Haemonetics ACP215 disposable glycerolizing set has simplified glycerolization of both nonrejuvenated and rejuvenated RBC [15]. The procedure to deglycerolize RBC frozen with 40% W/V glycerol using the ACP215 reduces the glycerol concentration to <1%, reduces the level of PIPA solution used to biochemically modify the RBC prior to freezing, reduces the WBC to a mean of 1 X 10 7 in the unit, and reduces the plasma level to less than 5% with less than 1% hemolysis [18].…”

mentioning

confidence: 99%

Commentary on the preservation of human RBC, platelets, plasma clotting proteins and peripheral blood mononuclear hematopoietic stem cells by freezing and storage in a 80 C mechanical freezer

Valeri¹,

Giorgio²

2016

Clin Case Rep Rev

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MA has been supported for the past 50 years by the U.S. Navy's Bureau of Medicine and Surgery, Naval Medical Research and Development Command, and the Office of Naval Research (ONR) to develop, test, and evaluate procedures to preserve blood and blood products to treat military and civilian personnel subjected to trauma and radiation injury. Human RBC have been successfully frozen at -80°C for at least 21 years; human platelets have been successfully frozen for at least 2 years; human plasma has been successfully frozen at -80°C for at least 14 years; and human peripheral blood mononuclear hematopoietic stem cells have been successfully frozen at -80°C for at least 1½ years. The 50 year experience at the NBRL, Boston, MA has demonstrated that frozen red blood cell cells, frozen platelets, and frozen plasma can be used to successfully treat wounded casualties without any adverse events.Extensive in vitro studies have been published together with in vivo studies in normal volunteers, patients with hematologic disorders, patients subjected to surgical procedures with and without cardiopulmonary bypass, and patients subjected to traumatic injuries [1][2][3]. In addition, studies were performed on dogs and baboons to assess the safety and therapeutic effectiveness of autologous and allogeneic blood products.During the past 50 years, the preservation of the safe and therapeutically effective viable and functional RBC, platelets, plasma clotting protein and peripheral blood mononuclear hematopoietic stem cells has been accomplished by freezing and storage in a -80°C mechanical freezer.Storage of blood products in a -80°C mechanical freezer produces a freezing rate of 2 to 3°C per minute.Human RBC have been successfully frozen with 40% W/V glycerol at -80°C for at least 21 years [4]; human platelets have been successfully frozen at -80°C with 6% DMSO for at least 2 years [5,6] human plasma has been successfully frozen at -80°C for at least 14 years [7] and human peripheral blood mononuclear hematopoietic stem cells have been successfully frozen at -80°C for at least 1½ years [8].The freezing rate of 2-3°C per minute which occurs with the storage of platelets in a -80°C mechanical freezer produces a bimodal population of human and baboon platelets. The bimodal population represents a population of platelets that circulates like platelets stored at room temperature and a population of platelets that functions shortly after transfusion like platelets stored at 4°C [9][10][11][12].The procedures to freeze human RBC with 40% W/V glycerol, AbstractFor the past 50 years, NBRL has evaluated the cryopreservation of RBC, platelets, peripheral blood pluripotential hematopoietic mononuclear adult stem cells, and plasma to treat military personnel subjected to traumatic injuries and those exposed to radiation injury.The research was funded by the US-Navy's Bureau of Medicine and Surgery, the Office of Naval Research and the Congress of the United States to provide frozen blood products to supplement the liquid preserved blood produc...

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