Automation of a fluorometric method for the determination of 2-deoxy-d-glucose

Vittorio, N; Oloff, Clarence M; Sellers, Donald

doi:10.1016/0003-2697(84)90065-4

Cited by 2 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…, reacts with a trap molecule to produce a stable radical product that can be evaluated (163). Other trapping procedures allow a radical to react with a detector molecule to yield a stable product that can then be evaluated using a 634 KOHEN AND NYSKA TOXICOLOGIC PATHOLOGY variety of techniques, such as hydroxylation of salicylic acid (101), the deoxyribose assay (30,259), the cytochrome c reduction assay for detection of superoxide radicals (146), and detection of nitric oxide radicals by colored end-product compounds (5). Figure 7 demonstrates alternative approaches for the direct determination of ROS.…”

Section: Methods For Determination Of Ros and Radicalsmentioning

confidence: 99%

Invited Review: Oxidation of Biological Systems: Oxidative Stress Phenomena, Antioxidants, Redox Reactions, and Methods for Their Quantification

2002

View full text Add to dashboard Cite

Reactive oxygen species (ROS) and other radicals are involved in a variety of biological phenomena , such as mutation, carcinogenesis , degenerative and other diseases, in ammation, aging, and development . ROS are well recognized for playing a dual role as deleterious and bene cial species. The objectives of this review are to describe oxidative stress phenomena , terminology, de nitions, and basic chemical characteristics of the species involved; examine the biological targets susceptible to oxidation and the defense mechanisms of the organism against these reactive metabolites; and analyze methodologies , including immunohistochemica l markers, used in toxicological pathology in the visualization of oxidative stress phenomena . Direct detection of ROS and other free radicals is dif cult, because these molecules are short-lived and highly reactive in a nonspeci c manner. Ongoing oxidative damage is, thus, generally analyzed by measuremen t of secondary products including derivatives of amino acids, nuclei acids, and lipid peroxidation . Attention has been focused on electrochemica l methods based on voltammetry measurements for evaluating the total reducing power of biological uids and tissues. This approach can function as a tool to assess the antioxidant-reducin g pro le of a biological site and follow changes in pathological situations. This review thus includes different topics essential for understanding oxidative stress phenomen a and provides tools for those intending to conduct study and research in this eld.

show abstract

Section: Methods For Determination Of Ros and Radicalsmentioning

confidence: 99%