Automaticity of the SN and A-V Junctional Tissue Before and After Chemical Denervation in the Dog

Chen, Jyh-Hong; Lien, Wen-Pin; Chen, Jin-Jer; Lin, Fang‐Yue; Yang, Yu-Zen

doi:10.1378/chest.95.3.653

Cited by 3 publications

(3 citation statements)

References 33 publications

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“…Several procedures to develop an SSS animal model have been reported, primarily involving physical or chemical impairment of the sinoatrial node (SAN). Physical methods of impairing the SAN include cryocoagulation of the sinus node area [5], radio frequency ablation [6], and right coronary artery ligation [7]. However, physical methods have significant limitations, including procedural intricacy, unwarranted complications, low success rates, and unsuitability for smaller animals.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Rat Model of Sick Sinus Syndrome Using Pinpoint Press Permeation

Zhong

Wang

Lian

et al. 2018

BioMed Research International

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Objective Sick sinus syndrome (SSS) is one of the most common causes of cardiac impairment necessitating pacemaker implantation. However, studies of SSS pathogenesis are neither comprehensive nor conclusive due to limited success in achieving a stable rat SSS model. Here, we modified pinpoint press permeation to establish a stable rat SSS model. Methods We randomly assigned 138 male Sprague-Dawley rats into three groups: normal control (n = 8), sham (n = 10), and SSS (n = 120). Postoperatively, the SSS group was further divided into SSSA (n = 40), SSSB (n = 40), and SSSC (n = 40), based on reduction in heart rates by 20–30%, 31–40%, and 41–50%, respectively. We also assessed histomorphological characteristics and hyperpolarization-activated cyclic nucleotide-gated cation channel 4 (HCN4) expression in the sinoatrial node (SAN) at 1, 2, 3, and 4 weeks after surgery. ResultsMortality was statistically higher in SSSC compared to SSSA and SSSB (7.5% versus 90.0% and 87.5%; P < 0.05). Heart rate in SSSA was gradually restored to preoperative levels by week 4 after surgery. In contrast, heart rate in SSSB was stable at 2–3 weeks after surgery. However, we observed that the tissues and cells in SAN were severely injured and also found a time-dependent increase in collagen content and atrium myocardium in SSSB. HCN4 expression was significantly reduced at all 4 time points in SSSB, with statistically significant differences among the groups (P < 0.01). ConclusionWe successfully developed a rat SSS model that was sustainable for up to 4 weeks.

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Section: Introductionmentioning

confidence: 99%

“…Numerous attempts to establish experimental animal SSS models have been recently reported [5–10]. However, a majority of these studies were performed with larger animals, such as pigs, rabbits, or canines.…”

Section: Introductionmentioning

confidence: 99%

Development of a Rat Model of Sick Sinus Syndrome Using Pinpoint Press Permeation

Zhong

Wang

Lian

et al. 2018

BioMed Research International

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“…Most researchers utilize the method of applying a cotton bud dipped in 20% formaldehyde onto the sinus node area (6). Other methods include ligating the artery supplying the sinus node with blood (10), cryocoagulation of the sinus node area (4), and radiofrequency catheter ablation of the sinus node area (6). Most of these methods have difficulties pinpointing the sinus node area, require large incisal openings, and have a low survival rate.…”

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confidence: 99%

Comparative study between original and traditional method in establishing a chronic sinus node damage model in rabbit

Wang

et al. 2012

Journal of Applied Physiology

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L. Comparative study between original and traditional method in establishing a chronic sinus node damage model in rabbit. J Appl Physiol 113: 1802-1808, 2012. First published August 16, 2012 doi:10.1152/japplphysiol.00480.2012.-Sick Sinus Syndrome is a common and refractory arrhythmia, needing further study in which setting up a credible sinus node damage model is important. To explore the feasibility and superiority of an original formaldehyde pinpoint pressing permeation (FPPP) method for building a chronic sinus node damage (CSND) model, 5 rabbits were chosen from 35 as a sham-operation group, and the remaining were randomly divided into two groups: the formaldehyde wet compressing (FWC) group, in which models were established by applying a cotton bud dipped in 20% formaldehyde onto the sinus node (SN) area, and the FPPP group, in which models were established by injecting formaldehyde into the SN area through a self-made pinpointing and injecting electrode. We found that in both groups, the HR at 2 h, 24 h, 1 wk, and 2 wk after modeling decreased compared with premodeling; sinoatrial conduction time, sinus node recovery time, and corrected sinus node recovery time were prolonged compared with premodeling. The indexes mentioned shortened by 2 wk after modeling compared with 2 h in the FWC group, whereas they were stable after modeling in the FPPP group. The modeling achievement ratio in the FPPP group was higher and the death rate was lower. Under light microscope, paraffin sections of the SN tissue and cells showed severe injury in both groups. The results indicate that the CSND models in rabbits can be successfully established by the FPPP method, with higher achievement ratio, lower death rate, better stabilization effect, and less damaging comparing with the traditional method. chronic sinus node damage; model establishing; novel method; electrophysiology THE SICK SINUS SYNDROME (SSS) is a clinically common and refractory arrhythmia, whose precise pathogenesis has not yet been clearly elucidated. Clinical and experimental studies have shown that the cardiac pacing and conducting dysfunction resulting from the damage of sinus node (SN) cells may be the direct pathological mechanism (1). Therefore, the setting up of a credible and stable sinus node damage model is most important in the experimental study of SSS. Various methods are used to establish animal sinus node damage models. Most researchers utilize the method of applying a cotton bud dipped in 20% formaldehyde onto the sinus node area (6). Other methods include ligating the artery supplying the sinus node with blood (10), cryocoagulation of the sinus node area (4), and radiofrequency catheter ablation of the sinus node area (6). Most of these methods have difficulties pinpointing the sinus node area, require large incisal openings, and have a low survival rate. Moreover, they are only used to establish acute models rather than chronic ones. We therefore created a more refined approach, the formaldehyde pinpoint pressuring permeation (FPPP) method, f...

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Tratamiento y pronóstico de la reagudización grave en la enfermedad pulmonar obstructiva crónica

Elizalde

Mateos

Walther

et al. 2001

Revista Clínica Española

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Automaticity of the SN and A-V Junctional Tissue Before and After Chemical Denervation in the Dog

Cited by 3 publications

References 33 publications

Development of a Rat Model of Sick Sinus Syndrome Using Pinpoint Press Permeation

Development of a Rat Model of Sick Sinus Syndrome Using Pinpoint Press Permeation

Comparative study between original and traditional method in establishing a chronic sinus node damage model in rabbit

Tratamiento y pronóstico de la reagudización grave en la enfermedad pulmonar obstructiva crónica

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