Automatic procedure for the microestimation of 2,3-diphosphoglycerate

Effect of 2,3-diphosphoglycerate on oxygen affinity of blood in sickle cell anemia

“…They were measured by an automated enzyme assay (20) in which the activity of phosphoglyceromutase is governed by the amount of DPG in samples added to an incubation mixture. It should be noted that both "free" and "hemoglobin-bound" DPG are measured by the assay system.…”

Hemoglobin Louisville (β42 (CD1) Phe→Leu): an unstable variant causing mild hemolytic anemia

“…with PCMB (see Methods) gave these results (values between parentheses represent the theoretical values for the normal P-chain): Lys 11.0 (11); His 8.7 (9); Arg 3.4 (3); Asp 13.6 (13); Thr 6.8 (7) ; Ser 5.7 (5); Glu 11.4 (11) ; Pro 6.5 (7); Gly 12. 6 Fig. 1 and Table I. acid compositions of these peptides are listed in Table II; all #-chain peptides were recovered except the core peptides T-10, T-11, and T-12, and the #T-2 peptide, which was not obtained sufficiently pure to allow an accurate determination.…”

“…Freshly collected blood samples were mailed in ice airmail special Received for publication 3 May 1971. delivery from Louisville, Ky., to Augusta, Ga. The level of 2,3-diphosphoglycerate (2,3-DPG)' was determined on two occasions; the method used was that described by Grisolia, Moore, Luque, and Grady (6). Hemolysates were prepared usually within 24 hr after collection by mixing 1 vol of washed, packed, red cells, 1 volume of distilled water, and 0.2 vol of carbon tetrachloride for 10 min.…”

Hemoglobin Chapel Hill or α2Asp→Glyβ2

“…This aspartyl residue is not involved in contacts between chains nor in the contact with the heine group. Its counterpart in the/~ chain, i.e, aspartyl residue in position 79, forms a salt bond to lysyl aThe levels of 2,3-DPG were determined by the method of Grisolia et al [16] and were 16.3 and 16.9 ~mol/gHb, respectively. blsolated from red cell hemolysate of the propositus by DEAE-Sephadex chromatography [4,5].…”