Aim:Manual analysis of neck kinematics is usually associated with measurement errors and it requires the use of software capabilities. Considering laboratory usage, software has been developed to solve the associated problems.Materials & methods:Fluoroscopic images taken from 78 women were used to design and evaluate the performance of the software. The software was implemented using C# language, according to the case-based reasoning technique.Results:The viewpoints of experts suggest accuracy of the software in tracking and calculations, which meets their information requirements.Conclusion:Using the software could help physiotherapists to accomplish their work in decreased time and with improved accuracy.