Automatic Liquid Handling for Life Science: A Critical Review of the Current State of the Art

Kong, Fanwei; Liang, Yuan; Zheng, Yuan F.; Cao, Weidong

doi:10.1177/2211068211435302

Cited by 182 publications

(84 citation statements)

References 95 publications

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“…Several commercial robotic workstations are available for various purposes, including Equator GX8 Dispenser from Labcyte (Sunnyvale, CA, USA), MicroSys from Genomic Solutions (Ann Arbor, MI, USA), sciFLEXARRAYER dispenser from Scienion (Berlin, Germany) and other systems [162]. These platforms have been used to isolate plasmid DNA, transform bacteria, pick colonies and screen for protein expression [162,163], and a video showing the operation procedure for automatic protein purification is available [164]. Automatic platforms can cost hundreds of thousands of dollars and require routine maintenance, and organizations commonly hire specialists to care for these automated platforms.…”

Section: High-throughput Robotic Platform For Protein Expression and mentioning

confidence: 99%

High-throughput recombinant protein expression in Escherichia coli : current status and future perspectives

Jia¹,

Jeon²

2016

Open Biol.

236

160

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The ease of genetic manipulation, low cost, rapid growth and number of previous studies have made Escherichia coli one of the most widely used microorganism species for producing recombinant proteins. In this post-genomic era, challenges remain to rapidly express and purify large numbers of proteins for academic and commercial purposes in a high-throughput manner. In this review, we describe several state-of-the-art approaches that are suitable for the cloning, expression and purification, conducted in parallel, of numerous molecules, and we discuss recent progress related to soluble protein expression, mRNA folding, fusion tags, post-translational modification and production of membrane proteins. Moreover, we address the ongoing efforts to overcome various challenges faced in protein expression in E. coli, which could lead to an improvement of the current system from trial and error to a predictable and rational design.

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Section: High-throughput Robotic Platform For Protein Expression and mentioning

confidence: 99%

High-throughput recombinant protein expression in Escherichia coli : current status and future perspectives

Jia¹,

Jeon²

2016

Open Biol.

236

160

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“…Most up-to-date robotic liquid handlers are able to carry out relatively accurate pipetting and transportation of labware (Kong , et al , 2012). Some of the more complex platforms integrate temperature control units, shakers, and detection devices on board.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Recent advances in DNA assembly technologies

2014

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DNA assembly is one of the most important foundational technologies for synthetic biology and metabolic engineering. Since the development of the restriction digestion and ligation method in the early 1970s, a significant amount of effort has been devoted to developing better DNA assembly methods with higher efficiency, fidelity, and modularity, as well as simpler and faster protocols. This review will not only summarize the key DNA assembly methods and their recent applications, but also highlight the innovations in assembly schemes and the challenges in automating the DNA assembly methods.

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“…Today, techniques and tools are available to aid in the crystallization of difficult targets. Automation in the form of robotic plate-based screening techniques or microfluidic platforms allow many more conditions to be sampled and for economical use (sub-microliter volumes) of the sample [27,28]. Furthermore, techniques in molecular biology and protein chemistry, including modification of the target by enzymes (“see below”) and protein engineering, can aid in the crystallization of difficult targets.…”

Section: Practical Considerations In Fbddmentioning

confidence: 99%

Fragment-Based Screening by Protein Crystallography: Successes and Pitfalls

Chilingaryan

Yin

Oakley

2012

IJMS

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Fragment-based drug discovery (FBDD) concerns the screening of low-molecular weight compounds against macromolecular targets of clinical relevance. These compounds act as starting points for the development of drugs. FBDD has evolved and grown in popularity over the past 15 years. In this paper, the rationale and technology behind the use of X-ray crystallography in fragment based screening (FBS) will be described, including fragment library design and use of synchrotron radiation and robotics for high-throughput X-ray data collection. Some recent uses of crystallography in FBS will be described in detail, including interrogation of the drug targets β-secretase, phenylethanolamine N-methyltransferase, phosphodiesterase 4A and Hsp90. These examples provide illustrations of projects where crystallography is straightforward or difficult, and where other screening methods can help overcome the limitations of crystallography necessitated by diffraction quality.

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Automatic Liquid Handling for Life Science: A Critical Review of the Current State of the Art

Cited by 182 publications

References 95 publications

High-throughput recombinant protein expression in Escherichia coli : current status and future perspectives

High-throughput recombinant protein expression in Escherichia coli : current status and future perspectives

Recent advances in DNA assembly technologies

Fragment-Based Screening by Protein Crystallography: Successes and Pitfalls

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