Automatic High Resolution Digitisation of Metaphase Cells for Aberration Scoring and Karyotyping

Stark, Margaret; Farrow, Scott; Mckie, Mark; Rutovitz, Denis

doi:10.1007/978-3-642-74738-0_4

Cited by 6 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This resulted in 8-bit images with a grey scale in which darker pixels were represented by higher values. For some of the initial experiments, additional cells were digitized fully automatically on the MRC Human Genetic Unit's FlP, which also produces 8-bit images with a grey scale in which darker pixels have higher values [Stark et al, 1989;Bayley et al, 1991]; subsequent image processing and. analysis was identical to that described for images digitized by Magiscan-2.…”

Section: Image Digitization and Pre-processingmentioning

confidence: 99%

Automatic scoring of sister chromatid exchanges by image analysis in a dose response experiment

García-Sagredo

Piper

Rutovitz

et al. 1994

Environ and Mol Mutagen

View full text Add to dashboard Cite

A system which automatically selects second division metaphases and then, automatically scores the number of seEs of each cell is described. In on initial set of experiments, the performance of the components of the system was measured using a doto set in which metophases had been visually classified as either 2nd division or other; and in 2nd division metaphoses, every SeE had been marked on a hard copy.seE scoring had a true positive rate of about 75% and a false positive rate of about 1.5 false SeEs per metaphase analyzed. Second division detection hod a true positive rate of 80% and a false positive rate of about 10% of the non-2nd division cells. Next, the overall system was compared to human visual scoring in a dose-response experiment by analyzing the effect of mitomycin C on human chromosomes scored visually by two observers and by the fully automatic scoring. Human visual scoring and machine analysis showed similar dose responses, but the variability between them was considerable.

show abstract

Section: Image Digitization and Pre-processingmentioning

confidence: 99%

Automatic scoring of sister chromatid exchanges by image analysis in a dose response experiment

García-Sagredo

Piper

Rutovitz

et al. 1994

Environ and Mol Mutagen

View full text Add to dashboard Cite

show abstract

“…Promising research is being carried out at the MRC Human Genetics Unit, Edinburgh, where a goal for many years has been a completely automated system. Recent experiments indicate that digitisation of metaphases located at low magnification can now be performed automatically at high magnification (Stark et al 1989). Previously an operator had to adjust the position and focus the metaphase manually before digitisation.…”

Section: Practical Future Possibilltlesmentioning

confidence: 99%

Automated cytogenetic analysis: accomplishments, present status and practical future possibilities

Lundsteen

Philip

1989

Clinical Genetics

View full text Add to dashboard Cite

During the past 25 years, much research has been put into the development of systems for automated cytogenetic analysis. Currently more than 10 chromosome analysis systems are commercially available at prices from USD 50000–200000. The Magiscan chromosome system (Joyce‐Loebl Ltd.) was the first system to be integrated into a clinical cytogenetic laboratory at Rigshospitalet in Copenhagen. From 1983–1987, 6752 cases were analysed with two systems which can do both metaphase finding and karyotyping, and 7036 karyotypes were produced with one “karyotyping‐only” system. The development of automated cytogenetics systems is continuing in order to improve their performance further.

show abstract

Automatic detection of fragile X chromosomes using an X centromere probe

et al. 1990

View full text Add to dashboard Cite

In order to score for the fragile X syndrome, blood samples are prepared with absorption stain labeling by in situ hybridisation of the X chromosome centromeres. Metaphases are located, digitised at high resolution, and segmented fully automatically. A three stage adaptive classification scheme for labeled X chromosomes is then applied. This consists of a simple box classifier to identify plausible X and false positive X chromosomes, followed by a quadratic discriminant classifier that is re-trained for each sample. The modal number of X chromosomes is then determined for each sample and used to refine the classification. A simple fragile site detector is applied to the distal portion of the detected X chromosome long arms. From the results we estimate computer and operator time requirements for a screening system in which the operator reviews only the apparently fragile X chromosomes detected by the computer.

show abstract

Automatic High Resolution Digitisation of Metaphase Cells for Aberration Scoring and Karyotyping

Cited by 6 publications

References 7 publications

Automatic scoring of sister chromatid exchanges by image analysis in a dose response experiment

Automatic scoring of sister chromatid exchanges by image analysis in a dose response experiment

Automated cytogenetic analysis: accomplishments, present status and practical future possibilities

Automatic detection of fragile X chromosomes using an X centromere probe

Contact Info

Product

Resources

About