Automatic full compensation of quantitative phase imaging in off-axis digital holographic microscopy

Abstract

An automatic method that fully compensates the quantitative phase measurements in off-axis digital holographic microscopy (DHM) is presented. The two main perturbations of the quantitative phase measurements in off-axis DHM are automatically removed. While the curvature phase flaw introduced by the microscope objective is avoided by the use of an optimized telecentric imaging system for the recording of the holograms, the remaining phase perturbation due to the tilt of the reference wave is removed by the automatic computation of a digital compensating reference wave. The method has been tested on both nonbiological and biological samples with and improving on the quality of the recovered phase maps.

Improved Carrier Frequency-Shifting Algorithm Based on 2-FFT for Phase Wrap Reduction

“…In optical metrology, two-dimensional (2D) phase-based techniques have been widely used in various measurement applications, such as deformation and vibration measurement and three-dimensional (3D) surface measurement [1][2][3]. In these applications, information is always encoded in a modulated fringe, which can be directly projected by a commercial projector or generated using the interference method [4][5][6].…”

Sequential Shift Absolute Phase Aberration Calibration in Digital Holographic Phase Imaging Based on Chebyshev Polynomials Fitting

“…Recently, a lot of physical and numerical methods have been proposed to compensate or calibrate the system phase aberrations. Using the telecentric configuration or tunable lens can introduce the phase aberrations into the reference beam, which will partially compensate the lower order phase aberrations [7], [8]. The double exposure method needs removal the specimen from reference beam in second exposure, and can compensate the total aberrations accurately [9].…”

Automatic compensation of phase aberrations in digital holographic microscopy based on sparse optimization

“…To ARTICLE scitation.org/journal/app name a few, by adding a tube lens, a telecentric configuration is formed, and therefore, the parabolic phase is physically compensated. [7][8][9] The tilt phase is further removed from the phase map by a spatial filtering. 9 Similarly, by inserting an electrically tunable/adjustable lens in the illumination path or in the reference arm, the parabolic phase factor or total phase distortions can be canceled out.…”

“…[7][8][9] The tilt phase is further removed from the phase map by a spatial filtering. 9 Similarly, by inserting an electrically tunable/adjustable lens in the illumination path or in the reference arm, the parabolic phase factor or total phase distortions can be canceled out. [10][11][12] Besides, a common-path interferometer with one singlecube beam splitter is also demonstrated to physically compensate phase aberrations.…”