Abstract:Background: The automatic segmentation of pseudohyphal cell-aggregates from brightfield microscopy images for counting forming cells is a challenging task due to the heterogeneous optical appearances of the cells as they may lie on different focal planes. The current cell counting method is based on a time-consuming manual counting of stained cells on a hemocytometer and in most cases, it represents estimates of low statistical significance due to the effort needed to prepare and analyze many samples. In this … Show more
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