Automatic artifact removal from GFR measurements

Shmarlouski, Anatoli; Shulhevich, Yury; Geraci, Stefania; Friedemann, Jochen; Gretz, Norbert; Neudecker, Sabine; Hesser, Jürgen; Stsepankou, Dzmitry

doi:10.1016/j.bspc.2014.06.010

Cited by 9 publications

(11 citation statements)

References 24 publications

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“…We used a three-compartment kinetic model with linear correction to determine the GFR in mice. This model covers the injection process, the plasma volume, and the interstitial compartment allowing further refinement by correcting for shifts observed occasionally during measurements ( 37 ). After injecting FITC-sinistrin into a mouse that was fed a control diet (control) for 14 days, the fluorescent signal quickly increased at the beginning and descends down to the initial baseline level as it gets filtered by the kidneys after approximately 90 min.…”

Section: Resultsmentioning

confidence: 99%

“…Other than determining serum BUN and creatinine levels as well as other excretory markers of renal function, these animal studies did not assess GFR as primary endpoint. In our preclinical study, we used the transcutaneous GFR system based on the fluorescent tracer FITC-sinistrin, which is a precise method for repeated measurements in conscious and unrestricted mice to calculate the GFR ( 21 , 22 , 35 , 37 ). Fresolimumab, a TGFβ neutralizing antibody did not elicit any positive effect on the GFR in a randomized control trial including 416 patients with diabetic kidney disease ( 71 ).…”

Section: Discussionmentioning

confidence: 99%

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Anti-Transforming Growth Factor β IgG Elicits a Dual Effect on Calcium Oxalate Crystallization and Progressive Nephrocalcinosis-Related Chronic Kidney Disease

Steiger

Grill

et al. 2018

Front. Immunol.

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Crystallopathies are a heterogeneous group of diseases caused by intrinsic or environmental microparticles or crystals, promoting tissue inflammation and scarring. Certain proteins interfere with crystal formation and growth, e.g., with intrarenal calcium oxalate (CaOx) crystal formation, a common cause of kidney stone disease or nephrocalcinosis-related chronic kidney disease (CKD). We hypothesized that immunoglobulins can modulate CaOx microcrystal formation and crystal growth and that therefore, biological IgG-based drugs designed to specifically target disease modifying proteins would elicit a dual effect on the outcome of CaOx-related crystallopathies. Indeed, both the anti-transforming growth factor (TGF)β IgG and control IgG1 antibody impaired CaOx crystallization in vitro, and decreased intrarenal CaOx crystal deposition and subsequent CKD in mice on an oxalate-rich diet compared to oxalate-fed control mice. However, the TGFβ-specific IgG antibody showed nephroprotective effects beyond those of control IgG1 and substantially reduced interstitial fibrosis as indicated by magnetic resonance imaging, silver and α-smooth muscle actin staining, RT-qPCR, and flow cytometry for pro-fibrotic macrophages. Suppressing interstitial fibrosis slowed the decline of glomerular filtration rate (GFR) compared to treatment with control IgG1 [slope of m = −8.9 vs. m = −14.5 μl/min/100 g body weight (BW)/day, Δ = 38.3%], an increased GFR at the end of the study (120.4 vs. 42.6 μl/min/100 g BW, Δ = 64.6%), and prolonged end stage renal disease (ESRD)-free renal survival by 10 days (Δ = 38.5%). Delayed onset of anti-TGFβ IgG from day 7 was no longer effective. Our results suggest that biological drugs can elicit dual therapeutic effects on intrinsic crystallopathies, such as anti-TGFβ IgG antibody treatment inhibits CaOx crystallization as well as interstitial fibrosis in nephrocalcinosis-related CKD.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Anti-Transforming Growth Factor β IgG Elicits a Dual Effect on Calcium Oxalate Crystallization and Progressive Nephrocalcinosis-Related Chronic Kidney Disease

Steiger

Grill

et al. 2018

Front. Immunol.

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“…The MPD Studio software package (Mannheim Pharma and Diagnostics GmbH)2 was used to generate the elimination kinetics curve of FITC‐sinistrin from which the t 1/2 of FITC‐sinistrin was calculated using the 1‐COM,5,10 2‐COM,1,10 3‐COM10,29 and 3‐COMB 2,10. The elimination rate constant (λ), an expression of GFR indexed to extracellular fluid volume, was calculated using the following equation.

λ false({minute}^{- 1} false) = t_{1 / 2} \times l n false(2 false)

where t 1/2 represents the excretion half‐life and ln (2) is the natural logarithm of 2, which is approximately 0.693.…”

Section: Methodsmentioning

confidence: 99%

Impact of choice of kinetic model for the determination of transcutaneous FITC‐sinistrin clearance in rats with streptozotocin‐induced type 1 diabetes

Ullah

Evans

et al. 2020

Clin Exp Pharma Physio

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Transcutaneous assessment of fluorescein isothiocyanate (FITC)-sinistrin clearance using an optical device was recently validated for determination of glomerular filtration rate (GFR) in conscious animals. In the current study, we compared four available kinetic models for calculating FITC-sinistrin clearance, to provide further insight into whether the choice of model might influence findings generated using this device. Specifically, we calculated the excretion half-life of FITC-sinistrin (minutes), rate constant (minute −1 ) and GFR indexed to bodyweight in control rats and rats with streptozotocin-induced diabetes across a 4-week experimental period using standard one-compartment (1-COM), two-compartment (2-COM) and three-compartment (3-COM) kinetic models (1-COM), and a three-compartment kinetic model with baseline correction (3-COMB). Glomerular hyperfiltration was detected in STZ-induced diabetic rats with the 2-COM or 3-COMB at day 14 and with the 3-COM at day 3 and 14 after induction of diabetes, but not at any time point using the 1-COM. From a theoretical perspective, we reasoned that the 3-COMB model provides a better estimate of t 1/2 than the other models. Linear regression analysis of data generated using the 3-COMB showed a significant relationship between blood glucose and calculated GFR at the day 14 (P = .004) and day 28 (P = .01) time points, and a strong tendency for a relationship at the day 3 time point (P = .06). We conclude that hyperfiltration is an early and sustained characteristic of STZ-induced diabetes in rats. Furthermore, we propose that the 3-COMB model provides the most valid t 1/2 for estimation of GFR via transcutaneous detection of FITC-sinistrin clearance. K E Y W O R D S FITC-sinistrin clearance, glomerular filtration rate, glomerular hyperfiltration, kidney function, kinetic models, streptozotocin diabetic rats, transcutaneous method | 1159 ULLAH et AL. How to cite this article: Ullah MM, Ow CPC, Evans RG, Hilliard Krause LM. Impact of choice of kinetic model for the determination of transcutaneous FITC-sinistrin clearance in rats with streptozotocin-induced type 1 diabetes. Clin Exp

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“…If movement artefact is still of concern, algorithms for artefact removal from obtained signals can be used (see Shmarlouski et al . for detailed discussion) …”

Section: Methodsmentioning

confidence: 99%

Transcutaneous measurement of glomerular filtration rate in small rodents: Through the skin for the win?

et al. 2015

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This methods in renal research article describes a new non-invasive technique to measure glomerular filtration rate in small animals. Furthermore, this technology has potential for broader use in renal research, such as in measuring the rate of clearance of albumin from the circulation in animals with proteinuria. ABSTRACT:Rodent models of renal physiology and pathology are crucial to our understanding of the molecular, histological and functional sequelae that contribute to kidney diseases. One of the most important measures of renal function is glomerular filtration rate (GFR). While the accurate determination of GFR is pivotal to understanding the progression of disease and/or the benefits of treatment strategies, in rodents the conventional methods for assessment of GFR are inconvenient and cumbersome, not the least because they involve stress and often anaesthesia. The legitimacy of assay-based assessment of plasma and urine markers of GFR in mice has also been heavily scrutinized for their insensitivity to minor declines in GFR and inaccurate detection of renal biomarkers. While infusion-based clearance methods of GFR assessment are thus the gold standard in terms of accuracy, they are limited by the fact that they are primarily non-recovery procedures. This presents a dilemma when trying to document the progression of renal disease, as these measures cannot be taken in the same experimental subject. Here we review a technique of transcutaneous measurement of fluorescein isothiocyanate-labelled sinistrin to calculate GFR in small rodents, using a non-invasive clearance device (NIC-Kidney Device). This is a recently validated non-invasive technique for measuring GFR in small rodents that allows for the real-time measurement of GFR in conscious animals, without the need for plasma and urine assays.

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Automatic artifact removal from GFR measurements

Cited by 9 publications

References 24 publications

Anti-Transforming Growth Factor β IgG Elicits a Dual Effect on Calcium Oxalate Crystallization and Progressive Nephrocalcinosis-Related Chronic Kidney Disease

Anti-Transforming Growth Factor β IgG Elicits a Dual Effect on Calcium Oxalate Crystallization and Progressive Nephrocalcinosis-Related Chronic Kidney Disease

Impact of choice of kinetic model for the determination of transcutaneous FITC‐sinistrin clearance in rats with streptozotocin‐induced type 1 diabetes

Transcutaneous measurement of glomerular filtration rate in small rodents: Through the skin for the win?

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