Automatic and renewable micro-solid-phase extraction based on bead injection lab-on-valve system for determination of tranexamic acid in urine by UHPLC coupled with tandem mass spectrometry

Fernandes, Sara R.; Barreiros, Luísa; Sá, Paula Morisco de; Miró, Manuel; Segundo, Marcela A.

doi:10.1007/s00216-021-03606-y

Cited by 7 publications

(2 citation statements)

References 32 publications

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“…μSPE has also followed the path of automation leading to different configurations and techniques based on on-line coupling with analytical techniques [70]. It is worth mentioning those methodologies based on lab-onvalve [71], 3D-printed microfluidic platforms [72], and even microchips [73,74] as miniaturized and automated applications of μSPE, joining the current trends in Analytical Chemistry.…”

Section: Micro-solid Phase Extractionmentioning

confidence: 99%

Ultramicroextraction as a miniaturization of the already miniaturized. A step toward nanoextraction and beyond

Azorín

Benedé

Chisvert

2023

J of Separation Science

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Miniaturization of the analytical process has been a widespread trend, and the sample preparation stage is not exempted from this downscaling. Since the introduction of microextraction techniques as miniaturization of classical extraction techniques, they have become one of the strengths in this field. However, some of the original approaches to these techniques did not fully cover all the current principles of Green Analytical Chemistry. For this reason, during the last years, much emphasis has been placed on reducing/eliminating toxic reagents, reducing the amount of the extraction phase, and searching for new greener, and more selective extractant materials. On the other hand, even though high accomplishments have been achieved, the same attention has not always been paid to reducing the amount of sample, which is essential when treating low‐availability samples such as biological samples, or in developing portable devices. In this review, we intend to give the readership an overview of the advances toward further miniaturization of microextraction techniques. Finally, a brief reflection is made on the terminology used, or that should, in our opinion, be used to term these new generation of miniaturized microextraction approaches. To this regard, the term, ‘ultramicroextraction’ is proposed to refer to those approaches beyond microextraction.

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Section: Micro-solid Phase Extractionmentioning

confidence: 99%

Ultramicroextraction as a miniaturization of the already miniaturized. A step toward nanoextraction and beyond

Azorín

Benedé

Chisvert

2023

J of Separation Science

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“…LOV accommodates sampling, online dilution and mixing by flow reversal, and online detection as it integrates an optical detection unit, enabling several operations in a single setup. This system has successfully been employed to set simpler, faster and less-laborious quantification of small molecules, sorbent-based sample pretreatment, , and molecular recognition procedures. , Furthermore, considering the need of ensuring minimal stress and dilution during the analysis of organic NPs, the LOV platform emerges as a valuable alternative for NPs characterization as it offers (i) inert bore conduits ( ca . 1.0 mm), larger than conventional size-exclusion columns and ultrafiltration/dialysis membranes, (ii) low operation flow rates ( e.g ., 1 μL s –1 ), and (iii) short distance (10 mm) between the sampling port and the detection unit.…”

mentioning

confidence: 99%

Lab-on-Valve Automated and Miniaturized Assessment of Nanoparticle Concentration Based on Light-Scattering

et al. 2023

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Nanoparticles (NPs) concentration directly impacts the dose delivered to target tissues by nanocarriers. The evaluation of this parameter is required during NPs developmental and quality control stages, for setting dose–response correlations and for evaluating the reproducibility of the manufacturing process. Still, faster and simpler procedures, dismissing skilled operators and post-analysis conversions are needed to quantify NPs for research and quality control operations, and to support result validation. Herein, a miniaturized automated ensemble method to measure NPs concentration was established under the lab-on-valve (LOV) mesofluidic platform. Automatic NPs sampling and delivery to the LOV detection unit were set by flow programming. NPs concentration measurements were based on the decrease in the light transmitted to the detector due to the light scattered by NPs when passing through the optical path. Each analysis was accomplished in 2 min, rendering a determination throughput of 30 h–1 (6 samples h–1 for n = 5) and only requiring 30 μL (≈0.03 g) of NPs suspension. Measurements were performed on polymeric NPs, as these represent one of the major classes of NPs under development for drug-delivery aims. Determinations for polystyrene NPs (of 100, 200, and 500 nm) and for NPs made of PEGylated poly-d,l-lactide-co-glycolide (PEG–PLGA, a biocompatible FDA-approved polymer) were accomplished within 108–1012 particles mL–1 range, depending on the NPs size and composition. NPs size and concentration were maintained during analysis, as verified for NPs eluted from the LOV by particle tracking analysis (PTA). Moreover, concentration measurements for PEG–PLGA NPs loaded with an anti-inflammatory drug, methotrexate (MTX), after their incubation in simulated gastric and intestinal fluids were successfully achieved (recovery values of 102–115%, as confirmed by PTA), showing the suitability of the proposed method to support the development of polymeric NPs targeting intestinal delivery.

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Micro-solid-phase extraction

Bagheri,

Lee

2024

Integrated Analytical Systems

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Automatic and renewable micro-solid-phase extraction based on bead injection lab-on-valve system for determination of tranexamic acid in urine by UHPLC coupled with tandem mass spectrometry

Cited by 7 publications

References 32 publications

Ultramicroextraction as a miniaturization of the already miniaturized. A step toward nanoextraction and beyond

Ultramicroextraction as a miniaturization of the already miniaturized. A step toward nanoextraction and beyond

Lab-on-Valve Automated and Miniaturized Assessment of Nanoparticle Concentration Based on Light-Scattering

Micro-solid-phase extraction

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