Automated washing of human progenitor cells: evaluation of apoptosis and cell necrosis

Abstract

Overall, our data suggest that the automated washing procedure is safe and suitable for processing of thawed HPC-A products and can be daily used in clinical routine.

A new system for quality control in hematopoietic progenitor cell units before reinfusion in autologous transplant

“…In our study, we have observed a median percentage of viable CD45+/7‐AAD– and CD45+/CD34+/7‐AAD– cells of 54.93 and 80.55%, respectively. These results are in agreement with the data previously published by our group and by other authors 32‐34. Additionally, we have also observed a positive correlation between the number of total colonies obtained by seeding after thawing and washing procedures of 76 of 96 cryopreserved HPC‐A units and the median percentage of viable CD45+/CD34+/7‐AAD– cells determined through flow cytometric method (r = 0.45).…”

Automatic washing of thawed haematopoietic progenitor cell grafts: a preclinical evaluation

“…Using the Sepax‐2, Scerpa et al . obtained TNC and CD34 + recoveries of 91·9% and 86·7%, respectively . Rodriguez et al .…”

“…Importantly, similar to Scerpa et al . but unlike others , we defined the cell recovery (%) as the ratio between postwash and prefreeze data so the cell recovery takes into account the cell loss induced by freezing/thawing steps in addition to the washing procedure. Using the Sepax‐2, Scerpa et al .…”

“…Furthermore, we made the choice to operate a sequential washing procedure to wash multiple HPC grafts. It is not clearly mentioned in the published studies how the multiple bag procedures were performed even though parallel washing of two bags at a time seems the standard . Although the TNC recovery, CD34 recovery or cell viability may show no statistically significant differences between sequential and parallel washing procedures, however, the function of the cells could be affected.…”

To Wash or Not to Wash? Comparison of Patient Outcome after Infusion of Cryopreserved Autologous Hematopoietic Stem Cells before and after the Replacement of Manual Washing by Bedside Thawing

“…This may soon change as automated cell washing options, such as Sepax (Biosafe, Eysins, Switzerland), are becoming widely available and have been reported to result in reduced side effects, good cell viability and recovery, as well as comparable neutrophil and platelet engraftment [3, 11, 17-19]. These automated procedures will almost certainly assume a more prominent role in the future but should be studied carefully with regard to the clinical outcome of the recipients.…”