Automated touch screen device for recording complex rodent behaviors

Mabrouk, Omar S.; Dripps, Isaac; Ramani, Subha; Chang, Chao‐Yuan; Han, Jialong; Rice, Kenner C.; Jutkiewicz, Emily M.

doi:10.1016/j.jneumeth.2014.05.004

Cited by 11 publications

(9 citation statements)

References 14 publications

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“…50,51 Webcams were connected via USB ports to analysis PCs running Matlab 2009 (Mathworks, Natick, MA, USA) software. Using the image acquisition toolbox in Matlab, data were collected via a custom designed motion monitoring program (Mark Dow, University of Oregon).…”

Section: Methodsmentioning

confidence: 99%

PKCβ Inhibitors Attenuate Amphetamine-Stimulated Dopamine Efflux

Zestos

Mikelman

Kennedy

et al. 2016

ACS Chem. Neurosci.

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Amphetamine abuse afflicts over 13 million people, and there is currently no universally accepted treatment for amphetamine addiction. Amphetamine serves as a substrate for the dopamine transporter and reverses the transporter to cause an increase in extracellular dopamine. Activation of the beta subunit of protein kinase C (PKCβ) enhances extracellular dopamine in the presence of amphetamine by facilitating the reverse transport of dopamine and internalizing the D2 autoreceptor. We previously demonstrated that PKCβ inhibitors block amphetamine-stimulated dopamine efflux in synaptosomes from rat striatum in vitro. In this study, we utilized in vivo microdialysis in live, behaving rats to assess the effect of the PKCβ inhibitors, enzastaurin and ruboxistaurin, on amphetamine-stimulated locomotion and increases in monoamines and their metabolites. A 30 min perfusion of the nucleus accumbens core with 1 μM enzastaurin or 1 μM ruboxistaurin reduced efflux of dopamine and its metabolite 3-methoxytyramine induced by amphetamine by approximately 50%. The inhibitors also significantly reduced amphetamine-stimulated extracellular levels of norepinephrine. The stimulation of locomotor behavior by amphetamine, measured simultaneously with the analytes, was comparably reduced by the PKCβ inhibitors. Using a stable isotope label retrodialysis procedure, we determined that ruboxistaurin had no effect on basal levels of dopamine, norepinephrine, glutamate, or GABA. In addition, normal uptake function through the dopamine transporter was unaltered by the PKCβ inhibitors, as measured in rat synaptosomes. Our results support the utility of using PKCβ inhibitors to reduce the effects of amphetamine.

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Section: Methodsmentioning

confidence: 99%

PKCβ Inhibitors Attenuate Amphetamine-Stimulated Dopamine Efflux

Zestos

Mikelman

Kennedy

et al. 2016

ACS Chem. Neurosci.

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“…Capacitance touch screens, such as the iPad TM or Android TM tablets have been used in animal behavior studies [ 21 ]. In addition to trajectory recording, tablets can track animal paws for gait analysis, and can thus be immensely helpful in subtle behavioral analysis.…”

Section: Discussionmentioning

confidence: 99%

An Infrared Touch System for Automatic Behavior Monitoring

et al. 2021

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Key requirements of successful animal behavior research in the laboratory are robustness, objectivity, and high throughput, which apply to both the recording and analysis of behavior. Many automatic methods of monitoring animal behavior meet these requirements. However, they usually depend on high-performing hardware and sophisticated software, which may be expensive. Here, we describe an automatic infrared behavior-monitor (AIBM) system based on an infrared touchscreen frame. Using this, animal positions can be recorded and used for further behavioral analysis by any PC supporting touch events. This system detects animal behavior in real time and gives closed-loop feedback using relatively low computing resources and simple algorithms. The AIBM system automatically records and analyzes multiple types of animal behavior in a highly efficient, unbiased, and low-cost manner.

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“…The light-dark test was performed to assess anxiety-like behaviors using MouseTrap on Galaxy Tab 2.10.1 (model# GT-P5113TSYXAR; sham n = 6; smTBI n = 6; rmTBI n = 6) [53]. An Android tablet screen was enclosed by opaque Plexiglas walls with a divider with a 3.2-cm 2 doorway that allowed free access between the light and the dark compartments.…”

Section: Methodsmentioning

confidence: 99%

Repeated Pediatric Concussions Evoke Long-Term Oligodendrocyte and White Matter Microstructural Dysregulation Distant from the Injury

et al. 2018

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Concussion or mild traumatic brain injury (mTBI) is often accompanied by long-term behavioral and neuropsychological deficits. Emerging data suggest that these deficits can be exacerbated following repeated injuries. However, despite the overwhelming prevalence of mTBI in children due to falls and sports-related activities, the effects of mTBI on white matter (WM) structure and its development in children have not been extensively examined. Moreover, the effect of repeated mTBI (rmTBI) on developing WM has not yet been studied, despite the possibility of exacerbated outcomes with repeat injuries. To address this knowledge gap, we investigated the long-term effects of single (s)mTBI and rmTBI on the WM in the pediatric brain, focusing on the anterior commissure (AC), a WM structure distant to the injury site, using diffusion tensor imaging (DTI) and immunohistochemistry (IHC). We hypothesized that smTBI and rmTBI to the developing mouse brain would lead to abnormalities in microstructural integrity and impaired oligodendrocyte (OL) development. We used a postnatal day 14 Ascl1-CreER: ccGFP mouse closed head injury (CHI) model with a bilateral repeated injury. We demonstrate that smTBI and rmTBI differentially lead to myelin-related diffusion changes in the WM and to abnormal OL development in the AC, which are accompanied by behavioral deficits 2 months after the initial injury. Our results suggest that mTBIs elicit long-term behavioral alterations and OL-associated WM dysregulation in the developing brain. These findings warrant additional research into the development of WM and OL as key components of pediatric TBI pathology and potential therapeutic targets.

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Automated touch screen device for recording complex rodent behaviors

Cited by 11 publications

References 14 publications

PKCβ Inhibitors Attenuate Amphetamine-Stimulated Dopamine Efflux

PKCβ Inhibitors Attenuate Amphetamine-Stimulated Dopamine Efflux

An Infrared Touch System for Automatic Behavior Monitoring

Repeated Pediatric Concussions Evoke Long-Term Oligodendrocyte and White Matter Microstructural Dysregulation Distant from the Injury

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