“…Additional modifications of this method that have since been proposed include the use of haemolysates in the cyanmethaemoglobin form which avoids errors caused by methaemoglobin and carbonmonoxyhaemoglobin derivatives (Betke et al, 1959) and variation in the optimum wavelength at which the samples are measured (Jonxis and Visser, 1956;Kristoffersen, 1961;Pembrey et al, 1972). Automated assay systems based on this method have also been developed (Cabannes and Schmidt-Beurrier, 1965;Brook et al, 1974). The most common currently used tests are those of Singer et al (1951b) and Betke et al (1959) giving values for the upper limit of normal of 2% and 1% respectively.…”