Automated small‐scale protein purification and analysis for accelerated development of protein therapeutics

Abstract

Small‐scale protein purification presents opportunities for accelerated process development of biotherapeutic molecules. Miniaturization of purification conditions reduces time and allows for parallel processing of samples, thus offering increased statistical significance and greater breadth of variables. The ability of the miniaturized platform to be predictive of larger scale purification schemes is of critical importance. The PerkinElmer JANUS BioTx Pro and Pro‐Plus workstations were developed as intuitive, flexible, and automated devices capable of performing parallel small‐scale analytical protein purification. Preprogrammed methods automate a variety of commercially available ion exchange and affinity chromatography solutions, including miniaturized chromatography columns, resin‐packed pipette tips, and resin‐filled microtiter vacuum filtration plates. Here, we present a comparison of microscale chromatography versus standard fast protein LC (FPLC) methods for process optimization. In this study, we evaluated the capabilities of the JANUS BioTx Pro‐Plus robotic platform for miniaturized chromatographic purification of proteins with the GE ӒKTA Express system. We were able to demonstrate predictive analysis similar to that of larger scale purification platforms, while offering advantages in speed and number of samples processed. This approach is predictive of scale‐up conditions, resulting in shorter biotherapeutic development cycles and less consumed material than traditional FPLC methods, thus reducing time‐to‐market from discovery to manufacturing.

Protocol for high-throughput cloning, expression, purification, and evaluation of bispecific antibodies

“…The Caliper LabChip GXII (PerkinElmer) is a Microcapillary Electrophoresis system used for rapid characterization of protein samples. The microfluidics chip technology automatically stains, electrophoretically separates, destains and analyzes protein samples from 96- or 384-well microplates ( LeSaout et al., 2016 ). The separation observed in nrMCE is analogous to that of nrSDS-PAGE, in which high molecular weight (MW) species (such as covalent aggregates) migrate at a slower rate compared to low-MW species (such as ½ mAb).…”