Automated single-cell manipulation and sorting by light trapping

Buican, Tudor N.; Smyth, Miriam J.; Crissman, Harry A.; Salzman, G.C.; Stewart, Carleton C.; Martin, John

doi:10.1364/ao.26.005311

Cited by 219 publications

(109 citation statements)

References 13 publications

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“…While hydrodynamic focusing is maintained for a macroscopic FACS machine, optical force is used for cell deflection of microfluidic FACS (figure 2). Buican et al (1987) pioneered the use of a deflection beam to separate cells and a propulsion beam to maintain the travelling path of separated cells up to several millimetres. Ozkan et al (2003b) designed a T-type microchannel junction for transporting cell solution and used VCSEL-driven OT to trap and deliver several target cells to a collection reservoir simultaneously.…”

Section: Active Nanomanipulationmentioning

confidence: 99%

Optical tweezers for single cells

Zhang

Liu

2008

J. R. Soc. Interface.

653

441

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Optical tweezers (OT) have emerged as an essential tool for manipulating single biological cells and performing sophisticated biophysical/biomechanical characterizations. Distinct advantages of using tweezers for these characterizations include non-contact force for cell manipulation, force resolution as accurate as 100 aN and amiability to liquid medium environments. Their wide range of applications, such as transporting foreign materials into single cells, delivering cells to specific locations and sorting cells in microfluidic systems, are reviewed in this article. Recent developments of OT for nanomechanical characterization of various biological cells are discussed in terms of both their theoretical and experimental advancements. The future trends of employing OT in single cells, especially in stem cell delivery, tissue engineering and regenerative medicine, are prospected. More importantly, current limitations and future challenges of OT for these new paradigms are also highlighted in this review.

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Section: Active Nanomanipulationmentioning

confidence: 99%

Optical tweezers for single cells

Zhang

Liu

2008

J. R. Soc. Interface.

653

441

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“…We know from the early work of Ashkin and Smith how colloidal particles can act as nonlinear media Smith et al, 1982͒: with optical forces pulling particles into bright field lines one can see how a modulation of the refractive index might occur in such systems. Optical binding is not only restricted to colloidal objects but it is also applicable to living cells ͑Buican et al, 1987;Metzger et al, 2005͒. Exploitation of optically assisted organization of cells is still at its infancy; however, the topic holds promise as the control of initial cell patterns dictates subsequent cell growth for tissue engineering, cell signaling studies, and other studies in synthetic biology ͑Akselrod et al, 2006͒. In the remainder of this review we discuss optical binding and provide an overview of the key experiments and theoretical approaches in this area. While optical trapping has been in existence for over 40 years, one must note that the field of optical binding is far from being mature or well developed: we elucidate upon the present results obtained and challenges this field faces as well as indicate some future approaches that will lead to new discoveries.…”

Section: Introductionmentioning

confidence: 99%

Colloquium: Gripped by light: Optical binding

2010

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͑Published 3 June 2010͒The light-matter interaction has been at the heart of major advances from the atomic scale right to the microscopic scale over the past four decades. Confinement by light, embodied by the area of optical trapping, has had a major influence across all of the natural sciences. However, an emergent and powerful topic within this field that has steadily merged but not gained much recognition is optical binding: the importance of exploring the optically mediated interaction between assembled objects that can cause attractive and repulsive forces and dramatically influence the way they assemble and organize themselves. This offers routes for colloidal self-assembly, crystallization, and organization of templates for biological and colloidal sciences. In this Colloquium, this emergent area is reviewed looking at the pioneering experiments in the field and the various theoretical approaches that aim to describe this behavior. The latest experimental studies in the field are reviewed and theoretical approaches are now beginning to converge to describe the binding behavior seen. Recent links between optical binding and nonlinearity are explored as well as future themes and challenges.

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“…Using one or more focused laser beams one can build stable optical traps which can be used as tools for micro manipulation of cells, parts of cells, bacteria, viruses, etc. (1,4,6,8).…”

mentioning

confidence: 99%

Experimental and theoretical investigations on the validity of the geometrical optics model for calculating the stability of optical traps

et al. 1991

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We have developed a computer program based on the geometrical optics approach proposed by Roosen to calculate the forces on dielectric spheres in focused laser beams. We have explicitly taken into account the polarization of the laser light and thd divergence of the laser beam. The model can be used to evaluate the stability of optical traps in a variety of different optical configurations.Our calculations explain the experimental observation by Ashkin that a stable single-beam optical trap, without the help of the gravitation force, can be obtained with a strongly divergent laser beam. Our calculations also predict a different trap stability in the directions orthogonal and parallel to the polarization direction of the incident light.Different experimental methods were used to test the predictions of the model for the gravity trap. A new method for measuring the radiation force along the beam axis in both the stable and instable regions is presented. Measurements of the radiation force on polystyrene spheres with diameters of 7.5 and 32 pm in a TEM,,-mode laser beam showed a good qualitative correlation with the predictions and a slight quantitative difference.The validity of the geometrical approximations involved in the model will be discussed for spheres of different sizes and refractive indices.

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Automated single-cell manipulation and sorting by light trapping

Cited by 219 publications

References 13 publications

Optical tweezers for single cells

Optical tweezers for single cells

Colloquium: Gripped by light: Optical binding

Experimental and theoretical investigations on the validity of the geometrical optics model for calculating the stability of optical traps

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