Automated segmentation and tracking for large-scale analysis of focal adhesion dynamics

Würflinger, Thomas; Gamper, Ivonne; Aach, Til; Sechi, Antonio

doi:10.1111/j.1365-2818.2010.03404.x

Cited by 23 publications

(27 citation statements)

References 27 publications

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“…Images were taken using the same microscope, camera and software and described above. Live-cell FA characteristics were measured as before (Hou et al, 2012;Wűrflinger et al, 2010) with the exception that FAs were grouped based on whether they were off or on collagen lines.…”

Section: Fluorescence Microscopymentioning

confidence: 99%

The Number of Lines a Cell Contacts and Cell Contractility Drive the Efficiency of Contact Guidance

2013

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Cell migration is an important biological function that impacts many physiological and pathological processes. Often migration is directed along various densities of aligned fibers of collagen, a process called contact guidance. However, cells adhere to other components in the extracellular matrix, possibly affecting migrational behavior. Additionally, changes in intracellular contractility are well known to affect random migration, but its effect on contact guidance is less known. This study examines differences in directed migration in response to variations in the spacing of collagen, non-specific background adhesion strength and myosin II-mediated contractility. Collagen was microcontact printed onto glass substrates and timelapse livecell microscopy was used to measure migration characteristics. Increasing the number of lines a cell contacts or decreasing contraction led to decreases in directionality, but speed changes were context dependent. This suggests that while cell migration speed is a biphasic function of contractility, directionality appears to be a monotonic, increasing function of contractility. Thus, increasing the number of lines a cell contacts or decreasing contractility degrades the contact guidance fidelity. Abstract:Cell migration is an important biological function that impacts many physiological and pathological processes. Often migration is directed along various densities of aligned fibers of collagen, a process called contact guidance. However, cells adhere to other components in the extracellular matrix, possibly affecting migrational behavior. Additionally, changes in intracellular contractility are well known to affect random migration, but its effect on contact guidance is less known. This study examines differences in directed migration in response to variations in the spacing of collagen, non-specific background adhesion strength and myosin II-mediated contractility. Collagen was microcontact printed

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Section: Fluorescence Microscopymentioning

confidence: 99%

The Number of Lines a Cell Contacts and Cell Contractility Drive the Efficiency of Contact Guidance

2013

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“…In [13], [14] we have proposed an algorithm for the tracking of focal adhesions (FAs) in wide-field microscopic images. It achieves a large number of consistently tracked FAs due to the correction of segmentation errors during tracking.…”

Section: B 2d Tracking Of Focal Adhesionsmentioning

confidence: 99%

“…Features such as FA area, integrated fluorescence and elongation have been compared to the automatically computed results yielding very similar curves [13], [14].…”

Section: ) Measurementsmentioning

confidence: 99%

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Fluorescence microscopic imaging and image analysis of the cytoskeleton

Herberich

Würflinger

Sechi

et al. 2010

2010 Conference Record of the Forty Fourth Asilomar Conference on Signals, Systems and Computers

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Cell stability and motility depends on a complex dynamic cytoplasmic scaffolding called the cytoskeleton. It is composed of actin filaments, intermediate filaments and microtubules, and interacts with neighbouring cells and the extracellular matrix via specialized adhesion sites -multimolecular complexes responsible for the transmission of mechanical force and regulatory signals. The dynamic behaviour of these subcellular structures in living cells can be analysed by fluorescence microscopy yielding series of 2D or 3D images. Towards a quantitative analysis, we present methods for the segmentation and motion estimation of cytoskeletal filaments as well as for the tracking of adhesion sites, allowing the quantification of cytoskeletal dynamics under different conditions.

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“…The motility of cells is influenced by so-called focal adhesions (FAs). The analysis of their dynamics requires the segmentation and tracking of FAs [63].…”

Section: Bioimagingmentioning

confidence: 99%

Challenges of medical image processing

et al. 2010

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In todays health care, imaging plays an important role throughout the entire clinical process from diagnostics and treatment planning to surgical procedures and follow up studies. Since most imaging modalities have gone directly digital, with continually increasing resolution, medical image processing has to face the challenges arising from large data volumes. In this paper, we discuss Kilo-to Terabyte challenges regarding (i) medical image management and image data mining, (ii) bioimaging, (iii) virtual reality in medical visualizations and (iv) neuroimaging. Due to the increasing amount of data, image processing and visualization algorithms have to be adjusted. Scalable algorithms and advanced parallelization techniques using graphical processing units have been developed. They are summarized in this paper. While such techniques are coping with the Kilo-to Terabyte challenge, the Petabyte level is already looming on the horizon. For this reason, medical image processing remains a vital field of research.

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Automated segmentation and tracking for large-scale analysis of focal adhesion dynamics

Cited by 23 publications

References 27 publications

The Number of Lines a Cell Contacts and Cell Contractility Drive the Efficiency of Contact Guidance

The Number of Lines a Cell Contacts and Cell Contractility Drive the Efficiency of Contact Guidance

Fluorescence microscopic imaging and image analysis of the cytoskeleton

Challenges of medical image processing

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