Automated Sample Preparation and Chromatographic Analysis: Determination of CGS 10787B and Related Compounds

Abstract

An automated method is described for analyzing biological samples originating from CGS 10787B drug disposition studies. The method incorporates a laboratory robot to prepare plasma and urine samples and a high performance liquid chromatographic system to simultaneously analyze for CGS 10787B, as well as metabolites and drug-related compounds (CGS 12094, CGS 17000, and CGS 17001). The robot allquots the biological sample, adds an internal standard, and performs all the steps necessary for the liquid-liquid extraction and concentration of the drug and related components, while operating unattended around the clock. Recovery and reproducibility assessments indicate good accuracy and precision for the method. The limits of detection for the method are 0.2 microgram/mL in plasma and 0.5 microgram/mL in urine, for all components.

Historical review of sample preparation for chromatographic bioanalysis: pros and cons

“…Several manufacturers introduced robotic workstations for sample preparation. Systems incorporating a centrifuge, a balance, and capping and uncapping capability were able to perform totally automated SPE, LLE, and PPT of biological samples [Turnell and Cooper, 1987;Cohen, 1987;Pivnichny et al, 1987;Mueller et al, 1987;Luders and Brunner, 1987;Schmidt and Dong, 1987;Timmoney et al, 1989]. Other significant SPE developments include encasing the sorbent in Teflon or glass fiber [Wells, 1999;Hempenius et al, 1999], slurry packed cartridges, the 96-well formatted SPE plate [Kaye et al, 1996], and the polymeric sorbent [Peng et al, 2000;Eichhold et al, 2000;Franc -ois-Bouchard et al, 2001;Fraizer et al, 2002].…”

Application of robotics In the clinical laboratory

“…Some of these have been used to automate a range of clinical analyses, e.g. enzyme immunoassay, drug analysis, UV-visible spectrophotometric assay and bioassay [2][3][4][5][6][7][8]. Dedicated robotic arms have also been used for specimen/reagent transfer in automatic clinical chemistry analysers such as the Instrumentation Laboratories Monarch and the Roche Cobas-Fara, and in sample preparation units such as the Tecan RSP 510 Sampler and Kemble Star 700 instrument.…”

“…Hands designed to insert and remove both push-on and screw-on types of stoppers on vessels. 6. Hands designed to grasp turntables.…”