Automated quantitation of peripheral blood neutrophil activation in patients with myocardial ischaemia

Leckie, Margaret J; Gomma, Abuzeid; Purcell, Ian; Nyawo, Brian; Dewar, A.; Okrongly, David; Burman, J. F.; Hooper, James; Barnes, Peter J.; Clague, Jonathan R.; Hansel, Trevor T.

doi:10.1016/j.ijcard.2003.04.063

Cited by 11 publications

(12 citation statements)

References 28 publications

(25 reference statements)

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“…In this sense, it must be noted that, using similar methods, increased neutrophil size and reduced myeloperoxidase content in the blood neutrophils have been recently reported in individuals with ischemic heart disease [13,16]. Neutrophil light scatter and light absorption and MPXI measured by the ADVIA 120 analyser were significantly increased in our ET patients.…”

Section: Discussionsupporting

confidence: 79%

“…Neutrophil light scatter and light absorption and MPXI measured by the ADVIA 120 analyser were significantly increased in our ET patients. In vitro studies have shown changes in neutrophil shape and lamellipods after stimulation with interleukin-8, resulting in a significant increase in the amount of neutrophil light scatter, which is attributable to neutrophil activation [13]. Thus, in the presence of changes in cell shape or size, the neutrophils have been shown to undergo an activated respiratory burst and upregulation of markers of activation such as myeloperoxidase and CD11b [3,13,16].…”

Section: Discussionmentioning

confidence: 99%

“…The leukocytes were characterized on the basis of their staining activity and their morphology in a two-dimensional peroxidase cytogram, in which the mean position of a neutrophil cluster on the x-axis reflects the amount of light absorption (myeloperoxidase activity), while the light scatter is plotted on the y-axis (cell size) [5,13]. The analyzer calculates a MPXI of the mean myeloperoxidase content in the neutrophil population, which is close to À10 to þ10 in healthy subjects.…”

Section: Automated Neutrophil and Platelet Activation Analysismentioning

confidence: 99%

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Automated assessment of the neutrophil and platelet activation status in patients with essential thrombocythemia

et al. 2011

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Neutrophil and platelet activation are consistently found in essential thrombocythemia (ET), but the techniques employed to demonstrate such abnormalities are complex. To ascertain whether the ADVIA 120 analyzer can be employed to assess neutrophil and platelet activation status in ET, 55 such patients and the same number of matched healthy individuals were studied and the results correlated with neutrophil CD11b and platelet P-selectin expressions measured by flow cytometry. Compared with controls, ET patients had significantly higher values of neutrophil myeloperoxidase index (MPXI), mean platelet volume (MPV), platelet distribution width (PDW), and platelet component distribution width, and significantly lower values of neutrophil lobularity index and mean platelet component (MPC). Patients with the JAK2 mutation had significantly lower values of MPC and higher values of MPV and PDW than those with wild-type allele. A positive correlation was observed between MPXI and neutrophil CD11b expression and a negative correlation between MPC and platelet P-selectin expression. The intensity of the agreement between the variables obtained by the two methods was moderate. These results support the possible value of MPC as surrogate parameter of platelet activation in ET.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Automated Neutrophil and Platelet Activation Analysismentioning

confidence: 99%

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Automated assessment of the neutrophil and platelet activation status in patients with essential thrombocythemia

et al. 2011

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“…Besides the blood dosage by ELISA method, the MPO content can be measured in the neutrophils as a degranulation index by flow cytometry in some hematologic analyzers 6,59 .…”

Section: Technical Considerations In Myeloperoxidase Measurementsmentioning

confidence: 99%

Mieloperoxidase e doença arterial coronariana: da pesquisa à prática clínica

Roman¹,

Wendland²,

Polanczyk³

2008

Arq. Bras. Cardiol.

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“…Heparin indukuje oslobađanje MPO iz leukocita i neutrofila aktivisanih u koagulacionoj kaskadi (37). ELISA metodom može se određivati koncentracija MPO u neutrofilima kao degranulacioni indeks protočnom citometrijom pomoću hematoloških analizatora (38). Posude sa uzorkom krvi za merenje aktivnosti MPO treba držati na niskoj temperaturi, a plazma se može konzervisati citratom, EDTA-om ili heparinom koji povećava koncentraciju MPO.…”

Section: Laboratorijsko Merenje Mpounclassified

Predictive significance of myeloperoxidase for the occurrence of postoperative vascular complications

Vučinić¹,

Radovanovic²,

Čanović³

et al. 2016

Medicinski casopis

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UVODNajčešće i najznačajnije komplikacije operativnog hirurškog lečenja su vaskularne što nalaže dalje istraživanje etioloških i faktora rizika za njihov nastanak. Delovanje mijeloperoksidaze povezuje infekcijskoinflamatorne procese sa vaskularnim patološkim procesima, a određivanje njene koncentracije u plazmi i metabolita nastalih njenim delovanjem ima prediktivni značaj s obzirom na povišenje njenog nivoa i do tri meseca pre samog vaskularnog insulta.Proces oksidativnog stresa je izuzetno značajan za nastanak endotelne disfunkcije i postoperativnih kardiovaskularnih komplikacija od kojih su najznačajnije miokardna ishemija, koronarna, sistemska arterijska i venska tromboza i posledične embolije. Ovo stanje je uzrokovano osnovnim patološkim supstratom i samom

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Automated quantitation of peripheral blood neutrophil activation in patients with myocardial ischaemia

Cited by 11 publications

References 28 publications

Automated assessment of the neutrophil and platelet activation status in patients with essential thrombocythemia

Automated assessment of the neutrophil and platelet activation status in patients with essential thrombocythemia

Mieloperoxidase e doença arterial coronariana: da pesquisa à prática clínica

Predictive significance of myeloperoxidase for the occurrence of postoperative vascular complications

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