Automated quantification of colonic crypt morphology using integrated microscopy and optical coherence tomography

Qi, Xin; Pan, Yao; Hu, Zhongliang; Kang, Wei; Willis, Joseph E.; Olowe, Kayode; Sivak, Michael; Rollins, Andrew M.

doi:10.1117/1.2993323

Cited by 21 publications

(18 citation statements)

References 67 publications

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“…OCT has strong translational potential for endoscopic imaging for identification of colonic pathologies [26-28] For FMI, besides the cathepsin-based contrast agents [23], Roney et al recently demonstrated that the legume lectin Ulex europaeus agglutinin I (UEA-1) binds the surfaces of adenomatous polyps in specimens of colorectal cancer from the APC Min mouse model. The carbohydrate α-L-fucose, which is over-expressed on the surfaces of polyps, facilitates the bond with UEA-1.…”

Section: Methodsmentioning

confidence: 99%

Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging

et al. 2009

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Optical coherence tomography (OCT) provides high-resolution, cross-sectional imaging of tissue microstructure in situ and in real-time, while fluorescence molecular imaging (FMI) enables the visualization of basic molecular processes. There are great interests in combining these two modalities so that the tissue's structural and molecular information can be obtained simultaneously. This could greatly benefit biomedical applications such as detecting early diseases and monitoring therapeutic interventions. In this research, an optical system that combines OCT and FMI was developed. The system demonstrated that it could co-register en face OCT and FMI images with a 2.4 × 2.4 mm field of view. The transverse resolutions of OCT and FMI of the system are both ~10 μm. Capillary tubes filled with fluorescent dye Cy 5.5 in different concentrations under a scattering medium are used as the phantom. En face OCT images of the phantoms were obtained and successfully co-registered with FMI images that were acquired simultaneously. A linear relationship between FMI intensity and dye concentration was observed. The relationship between FMI intensity and target fluorescence tube depth measured by OCT images was also observed and compared with theoretical modeling. This relationship could help in correcting reconstructed dye concentration. Imaging of colon polyps of APC min mouse model is presented as an example of biological applications of this co-registered OCT/FMI system.

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Section: Methodsmentioning

confidence: 99%

Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging

et al. 2009

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“…18,19 These algorithms were developed for OCT images of other organs to investigate the diagnostic power. 20,21 CADx algorithms will be more important when large numbers of images need to be interpreted and as OCT imaging speed increases. Zuluaga et al 13 reported a higher average epithelial intensity in abnormal tissue than in normal tissue in premenopausal women.…”

Section: Background and Introductionmentioning

confidence: 99%

Diagnostic efficacy of computer extracted image features in optical coherence tomography of the precancerous cervix

Kang

Tresser³

et al. 2010

Medical Physics

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Purpose: To determine the diagnostic efficacy of optical coherence tomography ͑OCT͒ to identify cervical intraepithelial neoplasia ͑CIN͒ grade 2 or higher by computer-aided diagnosis ͑CADx͒. Methods: OCT has been investigated as a screening/diagnostic tool in the management of preinvasive and early invasive cancers of the uterine cervix. In this study, an automated algorithm was developed to extract OCT image features and identify CIN 2 or higher. First, the cervical epithelium was detected by a combined watershed and active contour method. Second, four features were calculated: The thickness of the epithelium and its standard deviation and the contrast between the epithelium and the stroma and its standard deviation. Finally, linear discriminant analysis was applied to classify images into two categories: Normal/inflammation/CIN 1 and CIN 2/CIN 3. The algorithm was applied to 152 images ͑74 patients͒ obtained from an international study. Results: The numbers of normal/inflammatory/CIN 1/CIN 2/CIN 3 images are 74, 29, 14, 24, and 11, respectively. Tenfold cross-validation predicted the algorithm achieved a sensitivity of 51% ͑95% CI: 36%-67%͒ and a specificity of 92% ͑95% CI: 86%-96%͒ with an empirical two-category prior probability estimated from the data set. Receiver operating characteristic analysis yielded an area under the curve of 0.86. Conclusions:The diagnostic efficacy of CADx in OCT imaging to differentiate high-grade CIN from normal/low grade CIN is demonstrated. The high specificity of OCT with CADx suggests further investigation as an effective secondary screening tool when combined with a highly sensitive primary screening tool.

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“…6 and 10 and also in previous studies [15,44,45], crypt morphology patterns were correlated with colon pathologies. OCM promises to facilitate the detection of dysplasia or differentiation between hyperplasic and adenomatous polyps in surveillance colonoscopy.…”

Section: Discussionmentioning

confidence: 78%

Ultrahigh speed spectral-domain optical coherence microscopy

Lee

Liu

Sheikine

et al. 2013

Biomed. Opt. Express

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Abstract:We demonstrate a compact, ultrahigh speed spectral-domain optical coherence microscopy (SD-OCM) system for multiscale imaging of specimens at 840 nm. Using a high speed 512-pixel line scan camera, an imaging speed of 210,000 A-scans per second was demonstrated. Interchangeable water immersion objectives with magnifications of 10×, 20×, and 40× provided co-registered en face cellular-resolution imaging over several size scales. Volumetric OCM data sets and en face OCM images were demonstrated on both normal and pathological human colon and kidney specimens ex vivo with an axial resolution of ~4.2 µm, and transverse resolutions of ~2.9 µm (10×), ~1.7 µm (20×), and ~1.1 µm (40×) in tissue. In addition, en face OCM images acquired with high numerical aperture over an extended field-of-view (FOV) were demonstrated using image mosaicking. Comparison between en face OCM images among different transverse and axial resolutions was demonstrated, which promises to help the design and evaluation of imaging performance of Fourier domain OCM systems at different resolution regimes. 590-592 (1994). 4. M. Kempe, W. Rudolph, and E. Welsch, "Comparative study of confocal and heterodyne microscopy for imaging through scattering media," J. Opt. Soc. Am. A 13(1), 46-52 (1996). 5. Z. Ding, H. Ren, Y. Zhao, J. S. Nelson, and Z. Chen, "High-resolution optical coherence tomography over a large depth range with an axicon lens," Opt. Lett. 27(4), 243-245 (2002

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Automated quantification of colonic crypt morphology using integrated microscopy and optical coherence tomography

Cited by 21 publications

References 67 publications

Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging

Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging

Diagnostic efficacy of computer extracted image features in optical coherence tomography of the precancerous cervix

Ultrahigh speed spectral-domain optical coherence microscopy

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