Automated quantification of apoptosis in B‐cell chronic lymphoproliferative disorders: a prognostic variable obtained with the Cell‐Dyn Sapphire (Abbott) automated hematology analyzer

Fumi, Maurizio; Martins, Diana; Pancione, Ylenia; Sale, Silvia; Rocco, Vincenzo

doi:10.1111/ijlh.12198

Cited by 2 publications

(3 citation statements)

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“…After Mentz et coll . reported the ability of the Bayer H1 haematological analyser to detect the presence of apoptotic cells, 9 a similar capacity was indicated for most haemocytometers 10‐15 . The practical implication of these observations is that instrument‐specific cytographic abnormalities should induce BSR, thereby reducing the incidence of errors and diagnostic delays.…”

Section: Discussionmentioning

confidence: 70%

“…Even considering the known inaccuracy of manual cellular counting in the case of underpopulated cells, mostly, 18 the likelihood of IM increases progressively as the reactive lymphocytes percentage in the PB increases, even more so when the presence of these cells occurs associated with a relative lymphocytosis of more than 50% 28 . Contrary, the diagnostic value assigned to APCs concerns only the qualitative data of their presence, 11,13,14 while their quantification does not seem to have clinical‐prognostic significance.…”

Section: Discussionmentioning

confidence: 99%

“…These cells can cause some scattergram's abnormalities in most haematological analysers such as Bayer H1, 9 Beckman Coulter GenS, LH780 and DxH800, 10‐12 Abbott Cell‐Dyn Sapphire 13 and Sysmex XN‐1000 14 . Such changes in the instrumental cluster's morphology and the associated flags and alarms help select samples for BSR.…”

Section: Introductionmentioning

confidence: 99%

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Mindray BC‐6800 haematological analyser: 3D‐DIFF scattergram usefulness in infectious mononucleosis diagnosis

Gioia¹,

Fumi

Fiorini

et al. 2021

Int J Lab Hematology

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Introduction 3D‐DIFF scattergram of the Mindray BC‐6800 haematological analyser shows morphological abnormalities and lymphocyte cluster splitting related to the presence of reactive lymphocytes. This study aims to assess whether these cytographic changes are useful in detecting both activated and apoptotic lymphocytes, leading to an improvement in the laboratory diagnostic process of infectious mononucleosis. Methods Two hundred three samples with modified shape and doubled lymphocyte cluster of DIFF scattergram (study group) were divided into two different subgroups: with and, respectively, without serological evidence of ongoing IM. Activated and apoptotic cells in peripheral blood were counted by light microscopy or gating in the instrumental dot plots. Values of apoptotic cells counted by microscopy were compared with those resulting from gating. Results Samples with both shape change and doubled lymphocyte cluster had serological profiles according to the diagnosis of ongoing infectious mononucleosis. Blood smears review was positive for reactive lymphocytes in all 112 samples (100%). An underestimation of apoptotic cell count by light microscopy compared with the gating in the instrumental scatterplot was also observed (96 out of 112, 85.7%). Conclusion The additional lymphocyte cluster was significantly associated with activated and apoptotic lymphocytes in samples with serology suggesting ongoing infectious mononucleosis. Considering the significance of clue for infectious mononucleosis assigned to the apoptotic lymphocytes, a specific flag such as "apoptotic cells?" could be associate with the related cluster. Such a flag could be used for dedicated rules for smears review, thus increasing infectious mononucleosis detection in laboratories that do not usually practise instrumental cytograms observation.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

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Mindray BC‐6800 haematological analyser: 3D‐DIFF scattergram usefulness in infectious mononucleosis diagnosis

Gioia¹,

Fumi

Fiorini

et al. 2021

Int J Lab Hematology

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Smudge Cells in Chronic Lymphocytic Leukemia: Pathophysiology, Laboratory Considerations, and Clinical Significance

Marionneaux

Keohane

Lamanna

et al. 2021

Laboratory Medicine

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Chronic lymphocytic leukemia (CLL) is the most commonly encountered leukemia in the clinical laboratory. Cytoskeletal defects in CLL lymphocytes can result in the formation of up to 75% smudge cells (SCs) during blood film preparation. Failure to account for these damaged lymphocytes in the white blood cell (WBC) differential diminishes the accuracy and reproducibility of the results. Lacking clear practice standards on handling SCs in CLL, different laboratories may employ different methods to mitigate SC-induced errors. This review explores the pathophysiology of SCs, their effect on WBC differentials in CLL, and how these results can impact clinical decisions. The pros and cons of various SC corrective methods are described to assist laboratories in developing an optimized protocol to reduce errors and inconsistencies in WBC differentials. Finally, the potential utility of SC enumeration as an indicator of CLL prognosis is discussed in terms of laboratories with differing access to technology.

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Automated quantification of apoptosis in B‐cell chronic lymphoproliferative disorders: a prognostic variable obtained with the Cell‐Dyn Sapphire (Abbott) automated hematology analyzer

Abstract: In small laboratories without FCM and cytogenetic, smudge cells%, can be utilized as a preliminary diagnostic and prognostic tool in differential diagnosis of CLPD.

Cited by 2 publications

References 15 publications

Mindray BC‐6800 haematological analyser: 3D‐DIFF scattergram usefulness in infectious mononucleosis diagnosis

Mindray BC‐6800 haematological analyser: 3D‐DIFF scattergram usefulness in infectious mononucleosis diagnosis

Smudge Cells in Chronic Lymphocytic Leukemia: Pathophysiology, Laboratory Considerations, and Clinical Significance

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