Automated passive serial dilution microfluidic chip for calcium quantification based on the Arsenazo III method

Tazin, Nusrat; Patel, Dhruv; Lambert, Christopher Jordon; M. Shad, Mohammad H.; Campbell, Jeff; Gale, Bruce K.

doi:10.1039/d2sd00022a

Cited by 1 publication

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References 38 publications

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“…Arsenazo III (Sigma Chemical Co., St. Louis, Mo. ), is a chromophore that generates a colored complex with the interaction of Ca2+ ions and the intensity of the chromophore formed is proportional to the total calcium concentration, which can be quantified with a photometer at 650nm ( 29 ). Four roots from each experimental group with an average weight of 300 mg were selected and placed in an eppendorf tube with 250 µL of deionized water, and 2 for the control groups.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of alendronic acid solution in removal calcium hydroxide from root canals

Mejía-Haro,

Méndez-González,

Zavala-Alonso

et al. 2024

J Clin Exp Dent

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Background Intracanal medication is considered an alternative after instrumentation in the case of pulp necrosis. However, the elimination of this medication plays an important role in the obturation process, which is why various chelating solutions have currently been studied for this purpose. Therefore, this study aimed to analyze the irrigant based on alendronic acid (AA), which contains in its chemical structure functional groups capable of forming complexes with Ca2+ ions. Material and Methods 90 single-rooted teeth were crowned and standardized to a length of 13 mm. They were instrumented with a progressive K file (Dentsply Maillefer) from #15 to #30. Ca(OH)2 was placed as intracanal medication for 7 days. The roots were randomly divided into the following 4 experimental groups (n=13) according to the irrigant used: 2.25% NaClO, calcium suspension, 0.22% AA, and 10% citric acid; as well as negative (n=5; saline solution) and positive controls (n=5, 17% EDTA). The roots were divided longitudinally and the % of permeable dentinal tubules (% PDT) was determined by thirds (cervical, middle and apical), by analyzing the micrographs obtained by scanning electron microscope (SEM) and the imageJ program. The quantification of the remaining Ca2+ was determined using the Arsenazo III technique. Results The Kruskal Wallis test was used for the % of permeable dentinal tubules where a significant difference was determined for the different thirds ( p <0.005). In the apical third, citric acid and AA irrigants presented a median of 17.71 and 17.51 % PDT respectively. In relation to the quantification of the remaining total calcium, the lowest value was found with AA with a concentration of 4.83 mmol/L. Conclusions The 0.22% AA solution has the same capacity to remove Ca(OH)2 from the root canal walls as 17% EDTA and 10% citric acid. Key words: Alendronic acid, calcium hydroxide, citric acid, EDTA, irrigant solution,root canal.

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Section: Methodsmentioning

confidence: 99%

Efficacy of alendronic acid solution in removal calcium hydroxide from root canals

Mejía-Haro,

Méndez-González,

Zavala-Alonso

et al. 2024

J Clin Exp Dent

View full text Add to dashboard Cite

show abstract

Automated passive serial dilution microfluidic chip for calcium quantification based on the Arsenazo III method

Abstract: In this study, a valveless, resistance-based method was used to design and develop a passive microfluidic chip to implement an automated calcium assay in urine samples using the arsenazo III...

Cited by 1 publication

References 38 publications

Efficacy of alendronic acid solution in removal calcium hydroxide from root canals

Efficacy of alendronic acid solution in removal calcium hydroxide from root canals

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