Automated optimization of endoderm differentiation on chip

Abstract

Here we developed an automated mLSI chip platform with general analytical workflow for 3D stem cell cultures offers the optimization of in vitro generation of various cell types for cell replacement therapies.

Label-free imaging of 3D pluripotent stem cell differentiation dynamics on chip

“…1e , mean ± SD, n = 14936 nuclei in N = 20 cultures). However, experimental yields varied with an average of 90 ± 6% (mean ± SD, N r = 4) of double-expressing cells at the end of differentiation, as previously observed in the standard cell well plate and chip culture formats 15 .…”

Adipose microtissue-on-chip: a 3D cell culture platform for differentiation, stimulation, and proteomic analysis of human adipocytes

“…With the assisted self-aggregation mechanism in the cell culture chamber and retrieval of 3D cell cultures in a non-destructive manner, we added two new features to the mLSI design toolbox. 29,30 While the success rate for the formation of 96 3D cell culture processes was over 95%, the recovery rates for 3D cell cultures were 89% and thus similar to other, non-mLSI-based, microfluidic platforms. 47 hASCs formed highly reproducible 3D cell cultures on the chip, with a standard deviation of an equivalent diameter of 8 μm across six chip repeats.…”

“…27 Only recently mLSI technologies for automated 3D cell culture and analysis became available. [28][29][30] While a dimensional incompatibility of traditional mLSI fabrication techniques and 3D cell cultures has hampered progression of the technology, a 3D-printing-based fabrication approach overcomes this problem. 30 3D printing fabrication enable unit operations for handling 3D cell cultures on-chip as for example sample retrieval without destruction.…”

Label-free imaging of 3D pluripotent stem cell differentiation dynamics on chip

“…However, experimental yields varied with an average of 90% ± 6% (mean ± SD, N r = 4) of double-expressing cells at the end of differentiation, as previously observed in the standard cell well plate and chip culture formats. 16 …”