The development of a rapid, reliable and reproducible LC method for the determination and quantification of 13 polyphenols (gallic acid, protocatechuic aldehyde, gentisic acid, catechin, vanillinic acid, caffeic acid, vanillin, epicatechin, syringaldehyde, p-coumaric acid, ferulic acid, sinapic acid and resveratrol) in grapes and derived products is reported. The polyphenols were separated in less than 8 min. Employed was an RP-18e (100 mm 9 4.6 mm) monolithic type column. A gradient method with the following solvents was utilised for the chromatographic separation: A: 90% water, 2% acetic acid in methanol, and B: 90% methanol, 2% acetic acid in water. Two detectors in series were employed: a UV-Vis detector and a fluorescence excitation/emission detector. Influence of temperature (15-40°C) and solvent flow rate (2-5 mL min-1) on the separation were studied, and 25°C and 2.5 mL min-1 were found to be the optimum conditions. The relative standard deviations of the resulting peak areas, for both intra-and inter-experiments, were less than 2.4 and 2.6%, respectively. Finally, the developed method has been utilised for the quantification of the polyphenols in real samples.