Automated on-column derivatization of the antibiotics phosphinothricin and phosphinothricyl-alanyl-alanine with o∼phthalaldehyde and microbore co

Fiedler, Hans‐Peter; Plaga, Armin; Schuster, R.

doi:10.1016/s0021-9673(01)87089-8

Cited by 6 publications

(1 citation statement)

References 13 publications

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“…(168) Reversedphase separation was employed for all these separations because the OPA reaction is carried out in an alkaline buffer solution. Precolumn reactions with OPA for gentamicin, (169) sisomicin, (170) and phosphinothricin and its alanine analog (171,172) before reversed-phase separation have all been reported. Amikacin isomers were first adsorbed on a silica gel column before reaction with OPA; the derivatives were eluted with ethanol before separation by reversed-phase HPLC.…”

Section: Antibioticsmentioning

confidence: 99%

Chemical Reagents and Derivatization Procedures in Drug Analysis

Danielson

Gallagher

Bao

2008

Encyclopedia of Analytical Chemistry

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This article describes both pre‐ and postcolumn derivatization chemistry used in conjunction with either chromatography or capillary electrophoresis (CE) to facilitate the determination of drugs. Generally, only prederivatization is used in gas chromatography (GC), principally to enhance the volatility, temperature stability, and/or detectability. The GC section considers derivatization of drugs by reagent class: alkylation, acylation, and silylation. The GC sample‐handling section describes an approach that combines the extraction and derivatization steps together. Both pre‐ and postcolumn derivatization are common approaches for high‐performance liquid chromatography (HPLC) and many of these methods have been adapted for CE. Derivatization for HPLC is often directed toward aliphatic amines, carboxylic acids, or alcohols that are difficult to detect at low levels by absorbance, luminescence, or electrochemical means. In addition, small hydrophilic molecules upon prederivatization are often converted into larger more hydrophobic compounds, making reversed‐phase HPLC easier or even feasible. The HPLC section considers derivatization of drugs based on the following types: alkaloids, amines, antibiotics, barbiturates, carbonyl compounds and carboxylic acids, catecholamines, hydroxy compounds, steroids, and sulfur compounds. For the GC and HPLC sections, tables are included, giving the structures of the more important derivatizing agents, the analytes, and the corresponding reaction products. A brief rationale for derivatization chemistry with CE concludes this article. For CE, derivatization is often done to improve detectability as the path length for absorbance detection is very short and fluorescence can be effective using laser‐based systems.

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Section: Antibioticsmentioning

confidence: 99%

Chemical Reagents and Derivatization Procedures in Drug Analysis

Danielson

Gallagher

Bao

2008

Encyclopedia of Analytical Chemistry

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Chemical Reagents and Derivatization Procedures in Drug Analysis

Danielson¹,

Gallagher²,

Bao³

2000

Encyclopedia of Analytical Chemistry

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This article describes both pre‐ and postcolumn derivatization chemistry used in conjunction with either chromatography or capillary electrophoresis (CE) to facilitate the determination of drugs. Generally, only prederivatization is used in gas chromatography (GC), principallyto enhance the volatility, temperature stability, and/or detectability. The GC section considers derivatization of drugs by reagent class: alkylation, acylation, and silylation. The GC sample handling section describes an approach which combines the extraction and derivatization steps together. Both pre‐ and postcolumn derivatization are common approaches for high‐performance liquid chromatography (HPLC) and many of these methods have been adapted for CE. Derivatization for HPLC is often directed toward aliphatic amines, carboxylic acids, or alcohols that are difficult to detect at low levels by absorbance, luminescence, or electrochemical means. In addition, small hydrophilic molecules upon prederivatization are often converted into larger more hydrophobic compounds, making reversed‐phase HPLC easier or even feasible. The HPLC section considers derivatization of drugs based on type: alkaloids, amines, antibiotics, barbiturates, carbonyl compounds and carboxylic acids, catecholamines, hydroxy compounds, steroids, and sulfur compounds. For the GC and HPLC sections, tables are included giving the structures of the more important derivatizing agents, the analytes, and the corresponding reaction products. A brief rationale for derivatization chemistry with CE concludes this article. For CE, derivatization is often done to improve detectability since the path length for absorbance detection is very short and fluorescence can be effective using laser‐based systems.

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Retention prediction of o-phthalaldehyde amino acid derivatives in reversed-phase liquid chromatography

Jinno

Tanigawa

1987

Chromatographia

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Automated on-column derivatization of the antibiotics phosphinothricin and phosphinothricyl-alanyl-alanine with o∼phthalaldehyde and microbore co

Cited by 6 publications

References 13 publications

Chemical Reagents and Derivatization Procedures in Drug Analysis

Chemical Reagents and Derivatization Procedures in Drug Analysis

Chemical Reagents and Derivatization Procedures in Drug Analysis

Retention prediction of o-phthalaldehyde amino acid derivatives in reversed-phase liquid chromatography

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