Automated nucleated red blood cell count using the Mindray BC‐6800 hematology analyzer

Houyhongthong, V.; Nunphuak, W.; Sripatumtong, C.; Parnsamut, C; Ketloy, Chutitorn

doi:10.1111/ijlh.12876

Cited by 8 publications

(9 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The value of NRBC% examination in BM has been also suggested by a previous study, where a differential proliferation index of NRBC was proposed for early/low‐risk patients with MDS, showing increased proliferation, and advanced/high‐risk patients with MDS . Besides that, BC‐6800 hematology analyzer has been previously recognized for its accurate and reproducible NRBC counts in high‐value samples, such as patient monitoring samples used to determine the necessity of transfusion therapy in thalassemia patients . Thus, although NRBC% was integrated in the final SS, it was associated with a lower point‐system value due to its nonsignificant correlation coefficient.…”

Section: Discussionmentioning

confidence: 74%

“…18 Besides that, BC-6800 hematology analyzer has been previously recognized for its accurate and reproducible NRBC counts in high-value samples, such as patient monitoring samples used to determine the necessity of transfusion therapy in thalassemia patients. 19 Thus, although NRBC% was integrated in the final SS, it was associated with a lower point-system value due to its nonsignificant correlation coefficient.…”

Section: Discussionmentioning

confidence: 95%

See 1 more Smart Citation

“Bone marrow aspirate automated counts on hematology analyzers: formulating a scoring system based on hematology parameters, to discriminate reactive versus myelodysplastic syndrome‐related bone marrows”

Tsagarakis¹,

Paterakis²,

Papadhimitriou³

et al. 2019

Int J Lab Hematology

View full text Add to dashboard Cite

Introduction Diagnosis of myelodysplastic syndromes (MDS) is usually challenging. In this context, we have attempted to employ data derived from automated analysis of bone marrow (BM) samples as an ancillary tool for the discrimination between reactive marrow and MDS. Methods A total of 101 BM anticoagulated samples referred for flow cytometry (FCM) analysis on the clinical suspicion of MDS had been previously counted in a Mindray BC‐6800 hematology analyzer (testing set). Among them, 22/101 randomly selected BM samples (comparison set) had been also simultaneously counted by an Advia 2120 and a CELL‐DYN Sapphire hematology analyzer. Selected parameters obtained by Mindray BC‐6800 were retrospectively evaluated with ROC and regression analysis in an attempt to formulate a discriminative scoring system (SS) for MDS. This system was further evaluated in the comparison set. Results The diagnosis of MDS was established in 37/101 patients assessed (“MDS” group). Three patients were diagnosed with myelodysplastic/myeloproliferative neoplasm (MDS/MPN), while 61 revealed a “reactive” bone marrow (“RBM” group). Statistical analysis revealed significant differences in Hb, RDW‐CV%, NRBC%, and RET% values between the “MDS” and the “RBM” group. Specific cutoff values were then indicated and employed for the formulation of a SS of high sensitivity (86.84%) and specificity (86.89%). The encouraging performance characteristics of the proposed SS were also confirmed in the BM comparison set. Conclusion Automated BM counts on hematology analyzers contributed to the formulation of a SS for the screening discrimination between reactive and MDS BM fluids, which seems to be applicable and informative, regardless of the analyzer used.

show abstract

Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 95%

“Bone marrow aspirate automated counts on hematology analyzers: formulating a scoring system based on hematology parameters, to discriminate reactive versus myelodysplastic syndrome‐related bone marrows”

Tsagarakis¹,

Paterakis²,

Papadhimitriou³

et al. 2019

Int J Lab Hematology

View full text Add to dashboard Cite

show abstract

“…Pearson correlation analysis was used to evaluate the correlation of the different instruments’ test results (R, correlation coefficient). The consistency analysis of the results was evaluated by Passing-Bablok analysis and weighted Deming analysis, and the proportional and constant systematic errors were evaluated by the slope and intercept, with 95% confidence intervals (95% CI) [ 14 ]. The bias and 95% agreement limit of CRP value were evaluated by Bland-Altman analysis and calculated as the mean difference between the instruments.…”

Section: Methodsmentioning

confidence: 99%

Consistency Analysis of 3 Detection Systems for Measuring Serum C-Reactive Protein

et al. 2022

View full text Add to dashboard Cite

Background C-reactive protein (CRP) is an important clinical indicator. There are many methods and instruments for CRP measurement, and therefore the consistency of CRP values measured between instruments needs to be evaluated. This study aimed to compare the consistency of 3 serum CRP detection systems using turbidimetry. Material/Methods The consistency of CRP measured by 3 instruments, the Mindray BC-5390, Mindray BC-6800, and Johnson Vitros5600, was evaluated, and the consistency of blood routine measurement between the BC-5390 and BC-6800 was also evaluated. Pearson correlation analysis was used to evaluate the correlation of different instrument’s test results (R, correlation coefficient). The consistency of instruments was assessed by Passing-Bablok analysis and weighted Deming analysis. Results CRP data and route blood test data from 847 patients were used for analysis. The results showed that there were differences in the CRP values measured by the Mindray BC5390, Mindray BC6800, and Johnson Vitros5600 (χ 2 =78.573, P <0.001). The CRP measurement results of the BC5390 analyzer were consistent with those of the BC6800 analyzer (R=0.994, P <0.001) and Vitros5600 analyzer (R=0.983, P <0.001). However, there was a constant deviation in the CRP values measured by the BC-6800 and Vitros5600 analyzer (R=0.994, P <0.001). In the measurement of routine blood laboratory tests, the BC5390 analyzer and BC6800 analyzer were found to be interchangeable. Conclusions This study analyzed the consistency of CRP detection by 3 instruments, the Mindray BC-5390, Mindray BC-6800, and Johnson Vitros5600, and may provide a reference for the selection of CRP detection instruments.

show abstract

“…While manual microscopy is an established reference method for blood cell counts, it has a huge requirement for experienced manpower, and has low precision and efficacy, and is thus not suitable for the clinical setting (6,7). Automatic blood cell analyzers can combine resistance with flow cytometry to ensure accuracy and repeatability of clinical specimen results, and thus may be more suitable for clinical applications.…”

Section: Introductionmentioning

confidence: 99%

“…With advancements in instrument technology, the function of new blood cell analyzers has significantly increased, and the structure and principles are also more complex. To ensure that the performance of an automatic blood cell analyzer meets the clinical requirements, many committees and research institutes have developed standards and guidelines to facilitate the evaluation of instrument parameters and performance (5,7).…”

Section: Introductionmentioning

confidence: 99%

Detection of nucleated red blood cells using the Mindray BC-6800Plus hematology analyzer: a clinical performance evaluation

Wang¹,

Jiang²,

Wang³

et al. 2021

Ann Palliat Med

View full text Add to dashboard Cite

Background: With the development of instrument technology, the functions and detection methods of automatic blood cell analyzers have become more complex. To ensure optimal clinical applicability, it is crucial to select an automatic blood cell analyzer with excellent clinical detection performance. This study evaluated the latest Mindray BC-6800Plus automatic blood cell analyzer and assessed its performance in the detection of nucleated red blood cells (NRBCs).Methods: A total of 490 clinical blood samples were used to assess the performance of the instrument, including parameters such as precision, linearity, conformity rate of manual microscopic examination, carryover, and limit of quantitation.Results: The instrument showed a small carryover (≤0.02) and excellent linearity (R 2 ≥0.9986). The reproducibility of the sample tests was satisfactory, and the coefficient of variation (CV) of the test results [0.98-1.72% and 0.62-6.97% for white blood cells (WBCs) and NRBCs, respectively] were significantly lower than that declared by the manufacturer (2.5% and 20% for WBCs and NRBCs, respectively). Thus, the BC-6800Plus satisfies the requirements of clinical testing. Two separate Mindray BC-6800Plus machines were tested and found to be in good agreement with each other and with manual microscopy methods. Furthermore, WBC and NRBC counts were highly consistent with results obtained using the XN-9100 blood analyzer. Conclusions:The Mindray BC-6800Plus is an excellent analyzer that can provide timely and accurate reports for clinical laboratory detection of NRBC.

show abstract

Automated nucleated red blood cell count using the Mindray BC‐6800 hematology analyzer

Cited by 8 publications

References 18 publications

“Bone marrow aspirate automated counts on hematology analyzers: formulating a scoring system based on hematology parameters, to discriminate reactive versus myelodysplastic syndrome‐related bone marrows”

“Bone marrow aspirate automated counts on hematology analyzers: formulating a scoring system based on hematology parameters, to discriminate reactive versus myelodysplastic syndrome‐related bone marrows”

Consistency Analysis of 3 Detection Systems for Measuring Serum C-Reactive Protein

Detection of nucleated red blood cells using the Mindray BC-6800Plus hematology analyzer: a clinical performance evaluation

Contact Info

Product

Resources

About