2012
DOI: 10.1089/omi.2012.0013
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Automated Motif Discovery from Glycan Array Data

Abstract: Assessing interactions of a glycan-binding protein (GBP) or lectin with glycans on a microarray generates large datasets, making it difficult to identify a glycan structural motif or determinant associated with the highest apparent binding strength of the GBP. We have developed a computational method, termed GlycanMotifMiner, that uses the relative binding of a GBP with glycans within a glycan microarray to automatically reveal the glycan structural motifs recognized by a GBP. We implemented the software with … Show more

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Cited by 51 publications
(55 citation statements)
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References 43 publications
(65 reference statements)
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“…PNA did not recognize any glycan in which the disaccharide motif Galβ1-3GalNAc was sialylated or fucosylated [48].…”
Section: Pna Binding Specificitiesmentioning
confidence: 88%
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“…PNA did not recognize any glycan in which the disaccharide motif Galβ1-3GalNAc was sialylated or fucosylated [48].…”
Section: Pna Binding Specificitiesmentioning
confidence: 88%
“…It is evident from the glycan array data of Choletti et.al. [48] that SNA-I binds to the trisaccharide determinant NeuAc α2-6 Gal β1-4 GlcNAc when it is present on α1-3 linked Man of the diantennary structure but will not bind to the trisaccharide determinant when it is present on α1-6 linked Man. SNA-I does not bind NeuAc α2-6 Gal β1-4 Glc [48].…”
Section: Sna-i-binding Specificitiesmentioning
confidence: 99%
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“…These limitations could be addressed in future developments, such as new glycan array data with expanded glycan repertoires or varying densities (25,29) and the continued definition of motifs that account for complex specificities (21). In addition, we will continue to develop the analysis algorithms, for example, by building in additional statistical analyses or other, recently described approaches for analyzing glycan array data (31,32). The incorporation of new motifs, enhanced analysis methods, and new data from more complex glycan arrays should allow more accurate information to be obtained from the searches.…”
Section: Discussionmentioning
confidence: 99%
“…The simultaneous analyses of hundreds of unknown glycan structures using multiple analyses of replicate printed arrays before and after single or sequential enzyme digests will result in massive amounts of data that can only be handled computationally. Our initial approach to bioinformatics has developed out of our need to process data from the interaction of unknown GBPs with defined glycan arrays with the purpose to automate the discovery of GBP-binding motifs and define the glycan-binding specificity of GBPs (97). The algorithms used to perform these analyses will be used in developing more sophisticated software to automate the analyses of hundreds of glycans on SGMs.…”
Section: Fig 1 Glycan Determinants Defined By Glycan-binding Proteinsmentioning
confidence: 99%