Automated method for targeting analysis of prostanoids in human serum by on-line solid-phase extraction and liquid chromatography–mass spectrometry in selected reaction monitoring

Ferreiro‐Vera, Carlos; Mata-Granados, J.M.; Priego-Capote, Feliciano; Castro, M.D. Luque de

doi:10.1016/j.chroma.2011.03.049

Cited by 17 publications

(8 citation statements)

References 26 publications

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“…Ferreiro-Vera et al described simultaneous measurement of different inflammation biomarkers from human serum and human plasma and reported concentrations of PGF 2α and PGE 2 as 10.3 and 0.17 ng/ml in plasma [35] and 0.11 ng/ml for PGE 2 [36] in serum which are within our standard calibration range of 0.104 - 33.3 ng/ml. LOQs reported in their method as 46, 138 and 188 pg (on column) for PGF 2α , PGE 2 and PGD 2 were however found to be higher than our method.…”

Section: Discussionmentioning

confidence: 88%

“…These include PGD 2 , PGJ 2 , PGE 2 , 15-d-PGD 2 , PGA 2 , 15-d-PGJ 2 , PGF 2α , PGF 1α , 11β-PGF 2α , 6-keto-PGF 1α , and 11-d-TXB 2 . Several of these metabolites have been previously detected in different matrices such as cell cultures [32, 33], plasma/serum [35-37], urine [34, 39], rat kidney [43]. Masoodi et al [39] and Yue et al [44] reported measurements of prostanoids in rat brain or cortical tissue.…”

Section: Discussionmentioning

confidence: 99%

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Rapid and simultaneous quantitation of prostanoids by UPLC–MS/MS in rat brain

Shaik

Miller

Graham

et al. 2014

Journal of Chromatography B

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The metabolites of AA produced from the cyclooxygenase (COX) pathway, collectively termed as prostanoids, and from the CYP 450 pathway, eicosanoids, have been implicated in various neurodegenerative and neuroinflammatory diseases. This study developed a quantitative UPLC-MS/MS method to simultaneously measure 11 prostanoids including prostaglandins and cyclopentenone metabolites in the rat brain cortical tissue. Linear calibration curves ranging from 0.104 to 33.3 ng/ml were validated. The inter-day and intra-day variance for all metabolites was less than 15%. The extraction recovery efficiency and matrix (deionized water) effects measured at 12.5 ng/ml (750 pg on column) ranged from 88 to 100% and 3 to 14%, respectively, with % CV values below 20%. Additionally, applying the processing and extraction conditions of this method to our previous CYP450 eicosanoids method resulted in overall improvement in extraction recovery and reduction in matrix effects at low (0.417 ng/ml) and high (8.33 ng/ml) concentrations. In rat brain cortical tissue samples, concentrations of prostanoids ranged from 10.2 to 937 pmol/g wet tissue and concentration of eicosanoids ranged from 2.23 to 793 pmol/g wet tissue. These data demonstrate that the successive measurement of prostanoids and eicosanoids from a single extracted sample of rat brain tissue can be achieved with a UPLC-MS/MS system and that this method is necessary for evaluation of these metabolites as potential biomarkers in delineating their role in various neuroinflammatory and cerebrovascular disorders.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Rapid and simultaneous quantitation of prostanoids by UPLC–MS/MS in rat brain

Shaik

Miller

Graham

et al. 2014

Journal of Chromatography B

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“…1.B) [33][34][35]. In this configuration, two cartridges with the same sorbent are put in serial; hence the retention capability of the C8 SPE sorbent is evaluated by comparison of the amount of analyte retained in both cartridges.…”

Section: Recovery Factormentioning

confidence: 99%

Quantitative analytical method to evaluate the metabolism of vitamin D

Mena-Bravo

Ferreiro‐Vera²,

Priego-Capote

et al. 2015

Clinica Chimica Acta

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“…LC‐MS/MS is an excellent tool to monitor products of the eicosanoid degradation pathways as reported in the literature . This arrangement is especially useful because, apart from avoiding derivatization, the analysis can be automated by on‐line sample preparation and determination, particularly when sample preparation is based on SPE.…”

Section: Introductionmentioning

confidence: 99%

Targeted analysis of omega‐6‐derived eicosanoids in human serum by SPE‐LC‐MS/MS for evaluation of coronary artery disease

et al. 2013

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A targeted approach has been applied to quantitative analysis of eicosanoids derived from omega‐6 fatty acids in serum from individuals diagnosed with coronary artery disease (CAD). The target metabolites were series‐2 prostaglandins, thromboxane B2, hydroxy‐eicosatetraenoic acids, and hydroxyoctadecadienoic acids. The method was based on SPE‐LC‐MS/MS in selected reaction monitoring mode for highly selective and sensitive determination of the target eicosanoids. The combination of SPE and LC‐MS/MS involved the benefits from both direct analysis of serum without a step for protein precipitation and fully automation of the analysis. The method allowed comparison of omega‐6‐derived eicosanoids in serum from patients diagnosed with CAD and from control individuals. The effect of treatment with aspirin on the profile of the target compounds was evaluated through its incidence on the different pathways. Finally, the serum levels of the target metabolites in patients diagnosed with CAD were also statistically examined according to the severity of the coronary lesion stratified as stable angina, non‐ST‐elevation acute coronary syndrome, and acute myocardial infarction.

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Automated method for targeting analysis of prostanoids in human serum by on-line solid-phase extraction and liquid chromatography–mass spectrometry in selected reaction monitoring

Cited by 17 publications

References 26 publications

Rapid and simultaneous quantitation of prostanoids by UPLC–MS/MS in rat brain

Rapid and simultaneous quantitation of prostanoids by UPLC–MS/MS in rat brain

Quantitative analytical method to evaluate the metabolism of vitamin D

Targeted analysis of omega‐6‐derived eicosanoids in human serum by SPE‐LC‐MS/MS for evaluation of coronary artery disease

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