Automated Measurement of Microcirculatory Blood Flow Velocity in Pulmonary Metastases of Rats

Blueschke, Gert; Hanna, Gabi; Fontanella, Andrew N.; Palmer, Gregory M.; Boico, Alina; Min, Hooney; Dewhirst, Mark W.; Irwin, David; Zhao, Yue; Schroeder, Thies

doi:10.3791/51630

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…Vascular diameter and blood flow velocity, determined off-line from the captured video frames, were analyzed by a cross-correlation method using ImageJ. In brief, the luminal intensity of spatial structure in the image sequences was cross-correlated and blood flow velocity calculated by tracing moving FITC-labeled particles in the spatial distance between image locations by the time difference [velocity = distance(μm)/time(s)] ( 69 ). Volume flow (F) was estimated with the equation F = V × A (V, velocity; A, cross-sectional area of the vessel of πr 2 , where r = diameter/2).…”

Section: Methodsmentioning

confidence: 99%

VEGFA165 gene therapy ameliorates blood-labyrinth barrier breakdown and hearing loss

Zhang¹,

Hou²,

Wang³

et al. 2021

JCI Insight

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Millions of people are affected by hearing loss. Hearing loss is frequently caused by noise or aging, and often associated with loss of pericytes. Pericytes populate small vessels in the adult cochlea. However, their role in different types of hearing loss is largely unknown. Using an inducible and conditional pericyte depletion mouse model and noise-exposed mouse model, we show that loss of pericytes leads to marked changes in vascular structure, leading, in turn, to vascular degeneration and hearing loss. In vitro, using advanced tissue explants from pericyte fluorescence reporter models combined with exogenous donor pericytes, we show pericytes, signaled by endothelial growth factor isoform A165 (VEGFA165), vigorously drive new vessel growth in both adult and neonatal mouse inner ear tissue. In vivo, the delivery of an adeno-associated virus serotype 1 (AAV1)-mediated VEGFA165 viral vector to pericyte depleted or noise-exposed animals prevented and regenerated lost pericytes, improved blood supply, and attenuated hearing loss. These studies provide the first clear-cut evidence pericytes are critical for vascular regeneration, vascular stability, and hearing in adults. Restoration of vascular function in the damaged cochlea, including in noise-exposed animals, suggests that VEGFA165 gene therapy could be a new strategy for ameliorating vascular associated hearing disorders.

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Section: Methodsmentioning

confidence: 99%

VEGFA165 gene therapy ameliorates blood-labyrinth barrier breakdown and hearing loss

Zhang¹,

Hou²,

Wang³

et al. 2021

JCI Insight

View full text Add to dashboard Cite

show abstract

“…Vascular diameter and blood flow velocity, determined offline from the captured video frames, were analyzed by a cross-correlation method using ImageJ. In brief, the luminal intensity of spatial structure in the image sequences was cross correlated, and blood flow velocity was calculated by tracing the 3,3 0 -dioctadecyloxacarbocyanine perchlorateestained blood cells in the spatial distance between image locations by the time difference: 55 velocity Z distanceðmmÞ timeðsÞ…”

Section: Intravital Fluorescence Microscopymentioning

confidence: 99%

Acoustic Trauma Causes Cochlear Pericyte-to-Myofibroblast–Like Cell Transformation and Vascular Degeneration, and Transplantation of New Pericytes Prevents Vascular Atrophy

Hou

Neng

Zhang

et al. 2020

The American Journal of Pathology

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“…In order to protect the lungs from the outside environment, and to avoid collapse as well as stabilization, several groups fix a rigid transparent chest window on big mammals like cats [21,59], rabbits [23,58], rats [24,60,61], and less frequently mice [45]. A variant consists in the use of a soft window, generally a polymer membrane, sealed over the thoracotomy [25,26,56].…”

Section: Additional Techniquesmentioning

confidence: 99%

Intravital microscopy of the lung: minimizing invasiveness

Fiole

Tournier

2016

Journal of Biophotonics

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In vivo microscopy has recently become a gold standard in lung immunology studies involving small animals, largely benefiting from the democratization of multiphoton microscopy allowing for deep tissue imaging. This technology represents currently our only way of exploring the lungs and inferring what happens in human respiratory medicine. The interest of lung in vivo microscopy essentially relies upon its relevance as a study model, fulfilling physiological requirements in comparison with in vitro and ex vivo experiments. However, strategies developed in order to overcome movements of the thorax caused by breathing and heartbeats remain the chief drawback of the technique and a major source of invasiveness. In this context, minimizing invasiveness is an unavoidable prerequisite for any improvement of lung in vivo microscopy. This review puts into perspective the main techniques enabling lung in vivo microscopy, providing pros and cons regarding invasiveness.

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Automated Measurement of Microcirculatory Blood Flow Velocity in Pulmonary Metastases of Rats

Cited by 3 publications

References 18 publications

VEGFA165 gene therapy ameliorates blood-labyrinth barrier breakdown and hearing loss

VEGFA165 gene therapy ameliorates blood-labyrinth barrier breakdown and hearing loss

Acoustic Trauma Causes Cochlear Pericyte-to-Myofibroblast–Like Cell Transformation and Vascular Degeneration, and Transplantation of New Pericytes Prevents Vascular Atrophy

Intravital microscopy of the lung: minimizing invasiveness

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