Automated loading of discrete, microliter volumes of liquids into a miniature fast analyzer

Burtis, C. A.; Johnson, W. F.; Overton, J. B.

doi:10.1021/ac60342a054

Cited by 18 publications

(6 citation statements)

References 11 publications

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“…A centrifugal analyzer system that was developed and fabricated at the Oak Ridge National Laboratory (ORNL) was used in these studies. The integrated system consisted of a miniature centrifugal analyzer (23), an automated samplereagent loader (25), and a computerized data system. The data system was programmed by combining assembly language for the acquisition and storage of the data and FOCAL (Digital Equipment Corp.) for data retrieval, manipulation, and computation.…”

Section: Methodsmentioning

confidence: 99%

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Optimization of a kinetic method by response-surface methodology and centrifugal analysis and application to the enzymic measurement of ethanol

Burtis¹,

Bostick²,

Overton³

et al. 1981

Anal. Chem.

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Section: Methodsmentioning

confidence: 99%

“…A centrifugal analyzer with its multiple-sample processing (25) and automatic data handling capabilities is thus well suited for analytical method development using RSM. To demonstrate this compatibility, we have used RSM and centrifugal analysis to optimize a kinetic method for the enzymatic measurement of ethanol.…”

mentioning

confidence: 99%

Optimization of a kinetic method by response-surface methodology and centrifugal analysis and application to the enzymic measurement of ethanol

Burtis¹,

Bostick²,

Overton³

et al. 1981

Anal. Chem.

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“…A standard resoruf in reference solution was used in cuvette 2 for dynamic referencing (10). Aliquots of samples and reagent were automatically loaded into their respective chambers of the rotor using a mechanical pipetting device (11).…”

Section: Introductionmentioning

confidence: 99%

Development of laser-excited, fluorometric assays for the monitoring of toxic chemical exposure

Burtis¹,

Egan²,

Lee³

1985

Pure and Applied Chemistry

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-The niicrosomal mixed-function oxidase (MFO) enzyme system is involved in the inetabolisn of various chemical conpounds. Polycylic aromatic hydrocarbons and related compounds are metabolized by the cytochrome P-448 enzyme system, which contains MFOs. Induction of this MFO activity by toxic chemicals may be useful as an indicator of the toxicity of the inducer material. A centrifugal analyzer equipped with an argon-ion laser light source was used to quantitate cytochrome P-448 activity in mouse liver niicrosomes. The activity was induced by exposing the animals to different doses of liquids derived from a coalliquefaction process. The P-448 MFO activity was determined kinetically by measuring the rate at which the highly fluorescent compound, resoruf in, produced by oxidation of 7-ethoxyresorufin substrate, was formed. The 514.5-nm laser excitation beam was directed with a fiber optic bundle from the laser to the cuvettes of a specially designed rotor. The emitted fluorescence, filtered through a 560-nm cut-on filter, was monitored at 90° to the incident beam. A rotor, consisting of a black, acrylic plastic center body with transparent acrylic cuvette windows, was designed and fabricated to improve the fluorescence measurements. The use of a laser excitation source allows very low MFO activities to be measured; femtomole quantities of resoruf in can be determined.

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“…In addition, each well received a 15 «1 'flush' of physiological saline. The saline 'flush' insured complete specimen delivery and eliminated carry-over [11].…”

Section: Introductionmentioning

confidence: 99%

Detection of Hepatitis B Surface Antigen with the Miniature Centrifugal Fast Analyzer

Wenz¹,

Karmen²,

Feng³

1979

Vox Sanguinis

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A modified reversed passive hemagglutination test for the detection of hepatitis B surface antigen HBsAg is described. Sera and reagent cells coated with antibody to HBsAg (anti-HBs) are loaded separately into the rotor of a miniature centrifugal fast analyzer. The rotor is centrifuged briefly to transfer the components into its cuvettes. After mixing, the suspensions are allowed to stand at room temperature for 30 min, following which the rotor is again centrifuged and the absorbance of each cuvette is monitored. Cells suspended in serum containing HBsAg leave the light path more rapidly than cells suspended in sera free of antigen. The magnitude of change in absorbance varies directly with the concentration of the antigen. In 45 sera tested by the conventional V-plate technique, findings were as follows: 21 positive, 19 false positive and 5 negative. The automated procedure unequivocally differentiated the 21 positives; results for the false positive and negative specimens were identical and clearly distinguishable from the positive results. The automated procedure enhances specificity, offers equivalent sensitivity, and results that are quantitative and objective.

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Automated loading of discrete, microliter volumes of liquids into a miniature fast analyzer

Cited by 18 publications

References 11 publications

Optimization of a kinetic method by response-surface methodology and centrifugal analysis and application to the enzymic measurement of ethanol

Optimization of a kinetic method by response-surface methodology and centrifugal analysis and application to the enzymic measurement of ethanol

Development of laser-excited, fluorometric assays for the monitoring of toxic chemical exposure

Detection of Hepatitis B Surface Antigen with the Miniature Centrifugal Fast Analyzer

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